ENSG00000100784

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000418736 ENSG00000100784 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS6KA5 protein_coding protein_coding 3.333819 0.7865825 5.568422 0.03777813 0.2469555 2.807962 0.20342377 0.01372725 0.5043756 0.01372725 0.19332287 4.4382054 0.03947917 0.01926667 0.08966667 0.07040000 0.4321216067 0.0007171206 FALSE TRUE
ENST00000536315 ENSG00000100784 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS6KA5 protein_coding protein_coding 3.333819 0.7865825 5.568422 0.03777813 0.2469555 2.807962 1.21382982 0.00000000 1.8397776 0.00000000 0.51700353 7.5312080 0.26901667 0.00000000 0.33186667 0.33186667 0.0007171206 0.0007171206 FALSE TRUE
ENST00000556178 ENSG00000100784 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS6KA5 protein_coding nonsense_mediated_decay 3.333819 0.7865825 5.568422 0.03777813 0.2469555 2.807962 0.46509289 0.06138622 0.9547011 0.06138622 0.49471609 3.7563645 0.08172083 0.07360000 0.16676667 0.09316667 0.7760319462 0.0007171206 FALSE TRUE
ENST00000556594 ENSG00000100784 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS6KA5 protein_coding nonsense_mediated_decay 3.333819 0.7865825 5.568422 0.03777813 0.2469555 2.807962 0.05957982 0.06315944 0.1115003 0.06315944 0.11150030 0.7318438 0.02304583 0.07573333 0.02016667 -0.05556667 0.7581912393 0.0007171206   FALSE
ENST00000614987 ENSG00000100784 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS6KA5 protein_coding protein_coding 3.333819 0.7865825 5.568422 0.03777813 0.2469555 2.807962 0.13899895 0.08561226 0.1715229 0.01462878 0.01537247 0.9248838 0.11014167 0.11113333 0.03070000 -0.08043333 0.0558783666 0.0007171206 FALSE TRUE
ENST00000648062 ENSG00000100784 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS6KA5 protein_coding nonsense_mediated_decay 3.333819 0.7865825 5.568422 0.03777813 0.2469555 2.807962 0.96002253 0.50106308 1.5563397 0.05870710 0.14205921 1.6158238 0.40394583 0.64450000 0.28210000 -0.36240000 0.0082125437 0.0007171206 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100784 E001 67.2624252 0.0169875590 8.087140e-09 1.013820e-07 14 90847861 90870848 22988 - 1.599 2.062 1.560
ENSG00000100784 E002 37.0176236 0.0145215811 1.196934e-11 2.459656e-10 14 90870849 90871503 655 - 1.292 1.880 2.010
ENSG00000100784 E003 40.4280618 0.0275883413 6.244529e-01 7.407545e-01 14 90871504 90872073 570 - 1.492 1.597 0.357
ENSG00000100784 E004 22.5444089 0.0080119137 4.347761e-01 5.770740e-01 14 90872074 90872322 249 - 1.272 1.259 -0.046
ENSG00000100784 E005 21.7181196 0.0008913753 7.590065e-01 8.434707e-01 14 90873632 90873795 164 - 1.235 1.326 0.320
ENSG00000100784 E006 23.4391119 0.0008592903 8.648866e-01 9.169265e-01 14 90875201 90875360 160 - 1.276 1.326 0.175
ENSG00000100784 E007 22.5014634 0.0009557236 1.025070e-02 2.938276e-02 14 90890487 90890678 192 - 1.301 1.112 -0.669
ENSG00000100784 E008 0.8909948 0.0136208572 9.795463e-01 9.912563e-01 14 90893974 90894412 439 - 0.233 0.242 0.074
ENSG00000100784 E009 22.9728854 0.0007942422 6.976959e-04 2.890812e-03 14 90894413 90894583 171 - 1.316 1.030 -1.021
ENSG00000100784 E010 18.5149820 0.0010347575 1.541974e-04 7.658009e-04 14 90899329 90899422 94 - 1.238 0.844 -1.448
ENSG00000100784 E011 21.4677898 0.0009308649 1.151217e-04 5.914753e-04 14 90900108 90900241 134 - 1.295 0.928 -1.325
ENSG00000100784 E012 14.1754783 0.0035174945 3.206169e-03 1.087349e-02 14 90900611 90900674 64 - 1.125 0.794 -1.238
ENSG00000100784 E013 16.7303703 0.0014554795 3.182589e-02 7.551659e-02 14 90900675 90900736 62 - 1.177 0.998 -0.647
ENSG00000100784 E014 21.8574656 0.0009756276 5.083900e-01 6.438161e-01 14 90902808 90902969 162 - 1.256 1.260 0.014
ENSG00000100784 E015 11.4887076 0.0015045013 6.410681e-01 7.541647e-01 14 90906149 90906191 43 - 0.971 1.087 0.422
ENSG00000100784 E016 16.5262116 0.0015799683 7.202534e-01 8.149032e-01 14 90906192 90906299 108 - 1.134 1.160 0.092
ENSG00000100784 E017 0.2987644 0.0271902710 1.000000e+00   14 90911300 90911469 170 - 0.108 0.000 -10.198
ENSG00000100784 E018 1.8080138 0.0079835668 3.391905e-01 4.831185e-01 14 90919280 90919466 187 - 0.408 0.242 -1.062
ENSG00000100784 E019 19.8273579 0.0152475303 3.003231e-01 4.419946e-01 14 90920206 90920309 104 - 1.224 1.161 -0.224
ENSG00000100784 E020 17.8788913 0.0321128372 2.474604e-01 3.829585e-01 14 90923113 90923196 84 - 1.190 1.088 -0.364
ENSG00000100784 E021 0.2966881 0.0290785164 1.000000e+00   14 90923197 90923233 37 - 0.108 0.000 -10.198
ENSG00000100784 E022 0.2214452 0.0399603980 8.972303e-02   14 90939537 90939599 63 - 0.000 0.243 13.722
ENSG00000100784 E023 17.4301414 0.0013658385 2.559075e-01 3.928538e-01 14 90943078 90943185 108 - 1.169 1.113 -0.201
ENSG00000100784 E024 16.4608970 0.0563248386 4.797213e-01 6.180457e-01 14 90947435 90947550 116 - 1.143 1.097 -0.164
ENSG00000100784 E025 16.4724937 0.0028228156 3.622708e-03 1.207529e-02 14 90978306 90978394 89 - 1.181 0.888 -1.076
ENSG00000100784 E026 18.7536902 0.0011945374 1.288660e-03 4.938660e-03 14 90978395 90978524 130 - 1.235 0.928 -1.113
ENSG00000100784 E027 11.9887258 0.0135618135 1.070237e-02 3.045942e-02 14 91001088 91001159 72 - 1.060 0.739 -1.226
ENSG00000100784 E028 0.1817044 0.0427998348 8.991754e-02   14 91052323 91052462 140 - 0.000 0.242 13.718
ENSG00000100784 E029 0.0000000       14 91060018 91060331 314 -      
ENSG00000100784 E030 15.0501851 0.0184056005 5.175252e-02 1.122769e-01 14 91060332 91060641 310 - 1.134 0.929 -0.750