ENSG00000100664

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216554 ENSG00000100664 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5 protein_coding protein_coding 108.452 36.1372 182.1619 1.623577 2.932745 2.333345 29.465244 17.7727635 51.8991193 2.1461456 1.3867388 1.545508 0.32215833 0.488600000 0.284833333 -0.20376667 7.616114e-05 7.616114e-05 FALSE TRUE
ENST00000558800 ENSG00000100664 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5 protein_coding retained_intron 108.452 36.1372 182.1619 1.623577 2.932745 2.333345 1.273521 3.5071828 0.3751181 1.4204538 0.2266633 -3.191048 0.03070833 0.094133333 0.002033333 -0.09210000 1.455706e-03 7.616114e-05   FALSE
ENST00000559249 ENSG00000100664 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5 protein_coding retained_intron 108.452 36.1372 182.1619 1.623577 2.932745 2.333345 8.135916 0.5098427 18.9644204 0.5098427 5.0044855 5.189837 0.05686250 0.014200000 0.104400000 0.09020000 7.762344e-02 7.616114e-05   FALSE
ENST00000560338 ENSG00000100664 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5 protein_coding protein_coding 108.452 36.1372 182.1619 1.623577 2.932745 2.333345 5.407992 0.2161977 13.5581758 0.2161977 0.3221116 5.906499 0.03240000 0.006033333 0.074500000 0.06846667 2.775727e-02 7.616114e-05 FALSE FALSE
MSTRG.10223.5 ENSG00000100664 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF5 protein_coding   108.452 36.1372 182.1619 1.623577 2.932745 2.333345 51.591975 12.4073769 85.5805645 1.6755568 4.4286192 2.785091 0.44777083 0.348866667 0.469633333 0.12076667 3.438542e-01 7.616114e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100664 E001 0.6696487 2.465552e-02 8.618631e-01 9.149419e-01 14 103333544 103333663 120 + 0.177 0.213 0.336
ENSG00000100664 E002 3.3291295 6.235923e-02 8.709273e-01 9.210216e-01 14 103334189 103334191 3 + 0.585 0.551 -0.156
ENSG00000100664 E003 13.7476020 9.437435e-02 4.482866e-01 5.895876e-01 14 103334192 103334236 45 + 1.126 1.009 -0.425
ENSG00000100664 E004 131.7374396 1.533397e-02 9.056155e-09 1.125319e-07 14 103334237 103334260 24 + 2.129 1.684 -1.498
ENSG00000100664 E005 21.9112610 8.128651e-04 5.690017e-06 3.997911e-05 14 103334261 103334386 126 + 1.373 0.901 -1.699
ENSG00000100664 E006 16.5597786 8.047297e-03 4.087225e-04 1.807874e-03 14 103334387 103334388 2 + 1.257 0.825 -1.585
ENSG00000100664 E007 140.4179975 9.401776e-03 8.986577e-11 1.598273e-09 14 103334389 103334391 3 + 2.155 1.730 -1.426
ENSG00000100664 E008 498.1392199 8.190393e-03 4.181800e-06 3.027011e-05 14 103334392 103334597 206 + 2.675 2.456 -0.731
ENSG00000100664 E009 1.7735585 1.081837e-02 3.259298e-02 7.698767e-02 14 103334803 103334836 34 + 0.478 0.000 -12.496
ENSG00000100664 E010 1.3359641 5.938020e-02 8.717578e-02 1.708639e-01 14 103334861 103334893 33 + 0.399 0.000 -11.267
ENSG00000100664 E011 1.0393897 1.190131e-02 1.373713e-01 2.444127e-01 14 103334894 103334895 2 + 0.337 0.000 -11.680
ENSG00000100664 E012 2.3765117 6.651179e-03 9.539532e-02 1.834850e-01 14 103334896 103334948 53 + 0.545 0.213 -1.989
ENSG00000100664 E013 4.1383151 4.563962e-03 3.982430e-02 9.070312e-02 14 103334949 103335038 90 + 0.728 0.355 -1.783
ENSG00000100664 E014 3.6880353 8.263110e-03 7.238922e-02 1.473158e-01 14 103335039 103335062 24 + 0.686 0.355 -1.606
ENSG00000100664 E015 7.1436880 2.287206e-03 1.273489e-01 2.304439e-01 14 103335063 103335185 123 + 0.896 0.681 -0.854
ENSG00000100664 E016 18.1106960 1.061022e-03 9.988914e-04 3.954135e-03 14 103335186 103335480 295 + 1.281 0.934 -1.252
ENSG00000100664 E017 19.9984070 1.116380e-03 5.923584e-05 3.276512e-04 14 103335481 103335652 172 + 1.327 0.901 -1.541
ENSG00000100664 E018 681.6413993 4.098077e-03 6.053528e-05 3.341266e-04 14 103335653 103335791 139 + 2.796 2.662 -0.445
ENSG00000100664 E019 318.2200455 2.544321e-03 1.041365e-03 4.102126e-03 14 103335792 103335795 4 + 2.460 2.355 -0.351
ENSG00000100664 E020 791.7446273 3.173600e-03 6.575180e-06 4.555886e-05 14 103335796 103335932 137 + 2.861 2.728 -0.443
ENSG00000100664 E021 654.8398102 1.296709e-03 2.771535e-08 3.141432e-07 14 103336036 103336117 82 + 2.778 2.653 -0.414
ENSG00000100664 E022 3.1965180 1.107565e-02 3.391262e-02 7.957594e-02 14 103336118 103336178 61 + 0.638 0.213 -2.403
ENSG00000100664 E023 4.7514841 5.104488e-03 2.283798e-01 3.604889e-01 14 103336506 103336676 171 + 0.742 0.548 -0.835
ENSG00000100664 E024 363.4889885 1.525820e-04 2.625315e-08 2.990548e-07 14 103336677 103336684 8 + 2.522 2.405 -0.391
ENSG00000100664 E025 581.0571674 1.172178e-04 1.562281e-08 1.857122e-07 14 103336685 103336763 79 + 2.720 2.629 -0.302
ENSG00000100664 E026 605.5447311 1.013127e-04 4.810199e-09 6.297704e-08 14 103336764 103336849 86 + 2.738 2.646 -0.305
ENSG00000100664 E027 6.6738534 7.887721e-03 3.698639e-01 5.142723e-01 14 103336850 103337115 266 + 0.857 0.734 -0.486
ENSG00000100664 E028 628.6068125 4.614308e-04 6.671855e-09 8.499568e-08 14 103337116 103337227 112 + 2.756 2.653 -0.344
ENSG00000100664 E029 1.8467080 1.853203e-01 7.240400e-01 8.176882e-01 14 103337228 103337737 510 + 0.369 0.472 0.554
ENSG00000100664 E030 3.7870317 1.006801e-01 2.775658e-01 4.171514e-01 14 103338177 103338326 150 + 0.565 0.783 0.922
ENSG00000100664 E031 771.6732508 3.104721e-04 3.896489e-13 1.018739e-11 14 103338327 103338472 146 + 2.847 2.732 -0.384
ENSG00000100664 E032 872.0959451 1.141784e-04 8.781044e-03 2.573510e-02 14 103338735 103338893 159 + 2.881 2.855 -0.085
ENSG00000100664 E033 884.7950558 1.428325e-04 5.767060e-03 1.800099e-02 14 103339172 103339333 162 + 2.867 2.920 0.177
ENSG00000100664 E034 4.4921444 3.914092e-02 9.557537e-01 9.762425e-01 14 103339334 103339638 305 + 0.685 0.682 -0.016
ENSG00000100664 E035 986.5448661 1.070720e-04 6.463274e-05 3.540989e-04 14 103339639 103339803 165 + 2.911 2.978 0.223
ENSG00000100664 E036 1.6983276 8.305686e-03 6.535076e-01 7.639520e-01 14 103340404 103340426 23 + 0.369 0.462 0.503
ENSG00000100664 E037 964.1233864 8.430741e-05 1.720886e-08 2.031004e-07 14 103340427 103340561 135 + 2.896 2.984 0.295
ENSG00000100664 E038 469.6863557 1.265962e-04 1.416922e-02 3.860716e-02 14 103340963 103340969 7 + 2.591 2.652 0.203
ENSG00000100664 E039 1030.1984109 1.202835e-04 3.978943e-11 7.504967e-10 14 103340970 103341125 156 + 2.921 3.022 0.334
ENSG00000100664 E040 2234.8890375 1.443774e-04 3.578369e-12 8.015030e-11 14 103341126 103342158 1033 + 3.263 3.342 0.263
ENSG00000100664 E041 2532.1697178 5.314427e-03 6.224959e-07 5.403931e-06 14 103342159 103345025 2867 + 3.289 3.461 0.572