ENSG00000100650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000553369 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding protein_coding 160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 14.587919 1.474347 25.059841 1.4743467 4.0304368 4.07805280 0.08243750 0.00960000 0.12390000 0.114300000 3.414981e-02 4.722299e-12 FALSE FALSE
ENST00000554465 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding retained_intron 160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 10.697253 2.241420 13.760661 0.3430034 0.1668674 2.61269090 0.06340833 0.01746667 0.06823333 0.050766667 6.111165e-06 4.722299e-12 FALSE FALSE
ENST00000556184 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding retained_intron 160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 16.243255 11.612261 17.058600 1.2565621 0.8219450 0.55445394 0.10289583 0.08706667 0.08473333 -0.002333333 9.208880e-01 4.722299e-12 FALSE TRUE
ENST00000556330 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding retained_intron 160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 8.064813 5.649338 6.010982 2.8344357 0.2937901 0.08936574 0.05114583 0.03930000 0.02983333 -0.009466667 9.494726e-01 4.722299e-12 FALSE TRUE
ENST00000557154 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding protein_coding 160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 22.560478 43.365902 23.894768 3.7595409 1.6761341 -0.85959537 0.15282083 0.32803333 0.11856667 -0.209466667 4.722299e-12 4.722299e-12 FALSE TRUE
MSTRG.9806.11 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding   160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 11.615273 9.753863 10.166100 2.0499164 0.9062706 0.05966075 0.07587500 0.07340000 0.05030000 -0.023100000 4.079703e-01 4.722299e-12 FALSE TRUE
MSTRG.9806.4 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding   160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 25.739262 25.627788 37.734994 3.4379524 2.4552490 0.55801350 0.15831667 0.19240000 0.18733333 -0.005066667 9.609684e-01 4.722299e-12 TRUE TRUE
MSTRG.9806.5 ENSG00000100650 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF5 protein_coding   160.3875 132.9654 201.6841 12.20814 3.07358 0.6010095 29.543934 23.857934 44.356258 2.4130168 6.6177822 0.89438921 0.18350417 0.18003333 0.22036667 0.040333333 6.968340e-01 4.722299e-12 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100650 E001 0.000000       14 69726900 69727191 292 +      
ENSG00000100650 E002 0.000000       14 69766114 69766467 354 +      
ENSG00000100650 E003 0.000000       14 69766468 69766728 261 +      
ENSG00000100650 E004 0.481513 2.084842e-02 4.281459e-01 5.709504e-01 14 69766729 69767092 364 + 0.108 0.243 1.408
ENSG00000100650 E005 1.483236 1.519492e-02 4.800420e-01 6.183621e-01 14 69767093 69767111 19 + 0.328 0.459 0.730
ENSG00000100650 E006 2.882710 7.367310e-03 3.209086e-01 4.640747e-01 14 69767112 69767115 4 + 0.513 0.676 0.729
ENSG00000100650 E007 37.852811 1.144027e-03 2.319224e-01 3.647435e-01 14 69767116 69767141 26 + 1.556 1.637 0.273
ENSG00000100650 E008 100.161288 1.461558e-03 6.372364e-11 1.160570e-09 14 69767142 69767156 15 + 2.112 1.858 -0.854
ENSG00000100650 E009 439.940823 1.194583e-04 2.555687e-06 1.943432e-05 14 69767157 69767171 15 + 2.679 2.608 -0.235
ENSG00000100650 E010 481.582559 1.318886e-04 1.273342e-05 8.257216e-05 14 69767172 69767174 3 + 2.715 2.653 -0.206
ENSG00000100650 E011 497.435367 1.317975e-04 9.894848e-06 6.584528e-05 14 69767175 69767181 7 + 2.728 2.667 -0.205
ENSG00000100650 E012 543.540616 1.828595e-04 6.165190e-07 5.357200e-06 14 69767182 69767185 4 + 2.770 2.700 -0.233
ENSG00000100650 E013 791.805912 1.099099e-04 1.584525e-12 3.756520e-11 14 69767186 69767239 54 + 2.938 2.855 -0.275
ENSG00000100650 E014 570.642748 4.375180e-04 1.671509e-11 3.356251e-10 14 69767240 69767251 12 + 2.807 2.697 -0.366
ENSG00000100650 E015 373.235959 5.026673e-04 8.187554e-11 1.466214e-09 14 69767252 69767255 4 + 2.631 2.503 -0.427
ENSG00000100650 E016 127.639293 2.890505e-03 1.662240e-03 6.160624e-03 14 69767256 69767380 125 + 2.165 2.048 -0.393
ENSG00000100650 E017 3.815715 4.289363e-03 3.656667e-03 1.217392e-02 14 69767731 69767782 52 + 0.841 0.398 -1.983
ENSG00000100650 E018 3.815829 4.131773e-03 3.637298e-03 1.211902e-02 14 69767783 69767797 15 + 0.841 0.398 -1.983
ENSG00000100650 E019 8.965414 4.894840e-03 6.186304e-04 2.601849e-03 14 69767798 69767902 105 + 1.137 0.741 -1.496
ENSG00000100650 E020 5.803329 4.124791e-03 1.934064e-03 7.021899e-03 14 69767903 69767939 37 + 0.976 0.560 -1.689
ENSG00000100650 E021 7.144715 2.765533e-03 8.761201e-03 2.568767e-02 14 69767940 69767987 48 + 1.025 0.710 -1.218
ENSG00000100650 E022 5.609283 5.038199e-02 9.854823e-05 5.150750e-04 14 69767988 69768100 113 + 1.025 0.328 -3.086
ENSG00000100650 E023 8.055509 2.835089e-03 2.709567e-06 2.048688e-05 14 69768101 69768137 37 + 1.146 0.559 -2.307
ENSG00000100650 E024 1544.831623 8.588297e-05 1.387643e-03 5.266226e-03 14 69768138 69768282 145 + 3.200 3.181 -0.065
ENSG00000100650 E025 11.298628 3.135492e-02 3.639720e-01 5.083822e-01 14 69768283 69768603 321 + 1.137 1.019 -0.429
ENSG00000100650 E026 721.482400 6.696978e-04 2.610118e-03 9.094928e-03 14 69768604 69768612 9 + 2.830 2.892 0.206
ENSG00000100650 E027 1041.723623 5.686825e-04 1.068199e-01 2.008567e-01 14 69768613 69768674 62 + 3.004 3.037 0.111
ENSG00000100650 E028 12.993855 2.822048e-03 9.090289e-06 6.098870e-05 14 69768675 69768797 123 + 1.305 0.868 -1.592
ENSG00000100650 E029 1235.498759 4.387929e-04 5.184268e-04 2.228954e-03 14 69768798 69768896 99 + 3.066 3.123 0.190
ENSG00000100650 E030 95.004022 2.743174e-04 5.444483e-43 2.256982e-40 14 69768897 69769122 226 + 2.165 1.610 -1.868
ENSG00000100650 E031 43.056186 5.122978e-04 2.059475e-15 7.464345e-14 14 69769123 69769181 59 + 1.802 1.350 -1.545
ENSG00000100650 E032 880.812237 4.320194e-04 1.379835e-04 6.946737e-04 14 69769182 69769251 70 + 2.914 2.982 0.225
ENSG00000100650 E033 259.391799 3.962741e-03 1.275270e-02 3.533087e-02 14 69769252 69769510 259 + 2.455 2.364 -0.301
ENSG00000100650 E034 479.566653 4.051863e-03 1.142646e-07 1.148387e-06 14 69769511 69769745 235 + 2.757 2.578 -0.594
ENSG00000100650 E035 618.508675 4.880510e-03 5.951782e-05 3.290572e-04 14 69769746 69770042 297 + 2.851 2.714 -0.456
ENSG00000100650 E036 455.664952 2.696430e-03 7.852495e-02 1.571204e-01 14 69770043 69770447 405 + 2.628 2.690 0.207
ENSG00000100650 E037 188.420574 8.436002e-03 1.975485e-01 3.231501e-01 14 69770448 69770466 19 + 2.301 2.247 -0.182
ENSG00000100650 E038 905.181789 4.613760e-04 2.529512e-05 1.531752e-04 14 69770467 69770540 74 + 2.922 2.997 0.249
ENSG00000100650 E039 19.640823 9.816096e-04 2.883597e-06 2.165664e-05 14 69770881 69770994 114 + 1.457 1.088 -1.299
ENSG00000100650 E040 998.134159 1.004287e-03 2.502755e-04 1.176350e-03 14 69770995 69771105 111 + 2.961 3.039 0.259
ENSG00000100650 E041 1.646015 3.723689e-01 6.552495e-01 7.651794e-01 14 69771106 69771108 3 + 0.511 0.321 -1.038
ENSG00000100650 E042 433.414149 2.543688e-04 2.342539e-16 9.641255e-15 14 69771194 69771196 3 + 2.555 2.717 0.539
ENSG00000100650 E043 1545.905383 1.138317e-03 1.931001e-12 4.515768e-11 14 69771197 69771544 348 + 3.121 3.255 0.445
ENSG00000100650 E044 612.235441 1.496298e-03 9.289525e-16 3.521858e-14 14 69771545 69771916 372 + 2.685 2.881 0.651
ENSG00000100650 E045 186.788968 1.233886e-03 4.973772e-16 1.955721e-14 14 69771917 69772005 89 + 2.133 2.397 0.879