ENSG00000100601

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216489 ENSG00000100601 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH1 protein_coding protein_coding 20.57638 24.61702 23.51664 1.252772 1.397537 -0.06594675 3.682478 5.822570 2.336526 0.8655209 0.3041783 -1.3136053 0.17948750 0.23666667 0.10110000 -0.13556667 0.012684817 0.008511426 FALSE TRUE
ENST00000554097 ENSG00000100601 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH1 protein_coding processed_transcript 20.57638 24.61702 23.51664 1.252772 1.397537 -0.06594675 1.068156 1.397669 1.621759 0.4738836 0.1069614 0.2131190 0.05244167 0.05526667 0.06906667 0.01380000 0.714166216 0.008511426 FALSE FALSE
ENST00000557057 ENSG00000100601 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH1 protein_coding nonsense_mediated_decay 20.57638 24.61702 23.51664 1.252772 1.397537 -0.06594675 7.030365 9.880181 7.642278 0.6537382 0.2674308 -0.3701075 0.34180417 0.40103333 0.32696667 -0.07406667 0.163646908 0.008511426 TRUE TRUE
MSTRG.9987.1 ENSG00000100601 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH1 protein_coding   20.57638 24.61702 23.51664 1.252772 1.397537 -0.06594675 8.616442 7.313436 11.557826 0.7813847 1.3774539 0.6595252 0.41808333 0.29830000 0.48810000 0.18980000 0.008511426 0.008511426 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100601 E001 0.2924217 0.0290785164 1.321352e-01   14 77665651 77665689 39 - 0.244 0.000 -10.295
ENSG00000100601 E002 54.0227986 0.0300572488 2.259393e-03 8.028419e-03 14 77672404 77673074 671 - 1.509 1.826 1.076
ENSG00000100601 E003 273.0481168 0.0005225669 4.880201e-12 1.069203e-10 14 77673075 77673559 485 - 2.320 2.484 0.546
ENSG00000100601 E004 428.5029807 0.0002297543 3.845415e-01 5.286818e-01 14 77673560 77674102 543 - 2.628 2.621 -0.025
ENSG00000100601 E005 171.3700843 0.0002942831 2.204989e-01 3.510088e-01 14 77674103 77674241 139 - 2.245 2.219 -0.086
ENSG00000100601 E006 160.8874545 0.0003681329 1.218549e-01 2.225643e-01 14 77675656 77675794 139 - 2.222 2.185 -0.124
ENSG00000100601 E007 106.2436655 0.0006115894 9.771777e-01 9.897688e-01 14 77675795 77675849 55 - 2.018 2.025 0.024
ENSG00000100601 E008 124.3762504 0.0003207351 2.640570e-01 4.020027e-01 14 77679880 77679970 91 - 2.107 2.078 -0.097
ENSG00000100601 E009 10.4369233 0.0216585400 9.663581e-01 9.828771e-01 14 77691733 77691982 250 - 1.048 1.071 0.082
ENSG00000100601 E010 160.2254510 0.0003148356 5.556366e-03 1.743659e-02 14 77694738 77694900 163 - 2.240 2.169 -0.236
ENSG00000100601 E011 134.0439409 0.0013006352 2.437720e-02 6.059658e-02 14 77704369 77704477 109 - 2.161 2.090 -0.239
ENSG00000100601 E012 114.7195037 0.0006760378 1.006892e-02 2.893040e-02 14 77707822 77708023 202 - 2.101 2.020 -0.272