ENSG00000100591

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216479 ENSG00000100591 HEK293_OSMI2_2hA HEK293_TMG_2hB AHSA1 protein_coding protein_coding 288.111 331.9876 266.5684 29.14747 3.443763 -0.3166128 203.29356 254.84027 172.68660 14.087633 1.956862 -0.56141020 0.70363333 0.7719667 0.64786667 -0.12410000 9.367964e-03 2.443242e-42 FALSE TRUE
ENST00000554156 ENSG00000100591 HEK293_OSMI2_2hA HEK293_TMG_2hB AHSA1 protein_coding retained_intron 288.111 331.9876 266.5684 29.14747 3.443763 -0.3166128 34.85002 36.68441 36.96158 2.928193 2.389582 0.01085656 0.12260000 0.1110333 0.13890000 0.02786667 2.955146e-01 2.443242e-42 FALSE TRUE
ENST00000556866 ENSG00000100591 HEK293_OSMI2_2hA HEK293_TMG_2hB AHSA1 protein_coding retained_intron 288.111 331.9876 266.5684 29.14747 3.443763 -0.3166128 20.78459 0.00000 25.24259 0.000000 4.499706 11.30221590 0.07187083 0.0000000 0.09426667 0.09426667 2.443242e-42 2.443242e-42 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100591 E001 8.4700304 2.170571e-03 1.121124e-04 5.775486e-04 14 77457870 77457982 113 + 1.186 0.771 -1.549
ENSG00000100591 E002 0.7739347 1.484187e-02 3.325888e-01 4.762686e-01 14 77458032 77458032 1 + 0.348 0.181 -1.253
ENSG00000100591 E003 1.0684327 1.243480e-02 7.983528e-02 1.593098e-01 14 77458033 77458036 4 + 0.484 0.181 -1.989
ENSG00000100591 E004 209.1114949 1.298687e-03 9.855524e-01 9.949850e-01 14 77458037 77458092 56 + 2.302 2.307 0.017
ENSG00000100591 E005 1158.1471624 1.278156e-03 2.181269e-03 7.784005e-03 14 77458093 77458216 124 + 3.083 3.020 -0.208
ENSG00000100591 E006 1199.6290909 1.019165e-03 1.477519e-04 7.374050e-04 14 77458217 77458269 53 + 3.103 3.033 -0.232
ENSG00000100591 E007 1409.4829282 1.181678e-03 7.487841e-04 3.074378e-03 14 77459616 77459668 53 + 3.169 3.105 -0.213
ENSG00000100591 E008 1144.8058742 5.912625e-04 1.150821e-04 5.913035e-04 14 77459669 77459699 31 + 3.076 3.018 -0.195
ENSG00000100591 E009 1474.5062367 9.493172e-05 3.506503e-06 2.586019e-05 14 77459700 77459744 45 + 3.178 3.135 -0.141
ENSG00000100591 E010 1411.0460799 7.082707e-05 6.065429e-06 4.236698e-05 14 77459745 77459780 36 + 3.158 3.117 -0.134
ENSG00000100591 E011 1349.0177761 7.120041e-05 9.271076e-06 6.209792e-05 14 77459781 77459806 26 + 3.139 3.099 -0.133
ENSG00000100591 E012 6.9231020 3.350567e-03 2.339517e-02 5.856737e-02 14 77459807 77460214 408 + 1.035 0.771 -1.004
ENSG00000100591 E013 1736.9227725 9.573923e-05 2.069472e-04 9.937297e-04 14 77462160 77462242 83 + 3.241 3.210 -0.102
ENSG00000100591 E014 4.5185954 4.457125e-02 1.106708e-01 2.065205e-01 14 77462243 77462319 77 + 0.879 0.635 -0.987
ENSG00000100591 E015 2036.8290205 6.306235e-04 4.136893e-01 5.571034e-01 14 77462642 77462759 118 + 3.296 3.284 -0.038
ENSG00000100591 E016 17.2754659 1.094059e-02 7.700892e-14 2.227005e-12 14 77462760 77463101 342 + 1.574 0.841 -2.619
ENSG00000100591 E017 12.1197205 2.376248e-02 5.127746e-01 6.476716e-01 14 77464583 77464597 15 + 1.150 1.086 -0.233
ENSG00000100591 E018 1864.0172019 2.601235e-04 1.674441e-02 4.437543e-02 14 77464598 77464686 89 + 3.231 3.262 0.102
ENSG00000100591 E019 4.4410487 2.368831e-02 2.393506e-01 3.733666e-01 14 77464687 77464690 4 + 0.829 0.662 -0.676
ENSG00000100591 E020 1244.6009515 1.029695e-03 2.457865e-02 6.100849e-02 14 77465539 77465573 35 + 3.046 3.090 0.148
ENSG00000100591 E021 1830.2081981 3.042411e-04 1.158768e-09 1.696878e-08 14 77465574 77465667 94 + 3.193 3.268 0.248
ENSG00000100591 E022 1963.0948344 3.001849e-04 5.275050e-14 1.560254e-12 14 77468083 77468184 102 + 3.213 3.302 0.297
ENSG00000100591 E023 14.4547267 2.699382e-02 8.477271e-03 2.498009e-02 14 77468185 77468319 135 + 1.348 1.042 -1.088
ENSG00000100591 E024 1606.6116039 3.553443e-04 2.532550e-06 1.927054e-05 14 77468457 77468508 52 + 3.144 3.207 0.210
ENSG00000100591 E025 30.5563232 6.690197e-04 7.808468e-06 5.316983e-05 14 77468590 77468689 100 + 1.636 1.361 -0.944
ENSG00000100591 E026 20.7724760 8.643205e-04 5.179704e-06 3.673415e-05 14 77468690 77468760 71 + 1.507 1.174 -1.160
ENSG00000100591 E027 31.9965014 2.138908e-02 7.769338e-02 1.557575e-01 14 77468796 77468971 176 + 1.592 1.450 -0.486
ENSG00000100591 E028 23.2377062 1.415442e-02 1.069325e-01 2.010085e-01 14 77468972 77468985 14 + 1.454 1.324 -0.451
ENSG00000100591 E029 51.0417144 1.430289e-02 2.634864e-02 6.464472e-02 14 77468986 77469076 91 + 1.796 1.644 -0.514
ENSG00000100591 E030 2548.4917522 5.278811e-04 2.283236e-09 3.166793e-08 14 77469077 77469472 396 + 3.334 3.412 0.259