ENSG00000100528

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216416 ENSG00000100528 HEK293_OSMI2_2hA HEK293_TMG_2hB CNIH1 protein_coding protein_coding 154.7766 98.29962 205.7778 11.29539 2.531551 1.065753 11.89999 3.142063 23.00535 0.6309945 1.076475 2.8682277 0.06219167 0.0314000 0.1117000 0.0803000000 1.638445e-19 1.487047e-19 FALSE TRUE
ENST00000395573 ENSG00000100528 HEK293_OSMI2_2hA HEK293_TMG_2hB CNIH1 protein_coding protein_coding 154.7766 98.29962 205.7778 11.29539 2.531551 1.065753 31.50280 27.929859 34.56617 3.0505887 1.024774 0.3074535 0.23010417 0.2845667 0.1679333 -0.1166333333 1.487047e-19 1.487047e-19 FALSE FALSE
ENST00000553660 ENSG00000100528 HEK293_OSMI2_2hA HEK293_TMG_2hB CNIH1 protein_coding protein_coding 154.7766 98.29962 205.7778 11.29539 2.531551 1.065753 43.28224 29.388191 57.19478 5.1620094 3.725303 0.9604084 0.28396250 0.2952000 0.2779333 -0.0172666667 8.853777e-01 1.487047e-19 FALSE TRUE
ENST00000556113 ENSG00000100528 HEK293_OSMI2_2hA HEK293_TMG_2hB CNIH1 protein_coding protein_coding 154.7766 98.29962 205.7778 11.29539 2.531551 1.065753 42.82482 23.237537 62.10790 2.9498840 2.521508 1.4179312 0.26732083 0.2360667 0.3022333 0.0661666667 4.309681e-02 1.487047e-19 FALSE TRUE
ENST00000557659 ENSG00000100528 HEK293_OSMI2_2hA HEK293_TMG_2hB CNIH1 protein_coding nonsense_mediated_decay 154.7766 98.29962 205.7778 11.29539 2.531551 1.065753 23.70599 13.085927 28.11505 1.1805649 4.030109 1.1027372 0.14522083 0.1359000 0.1363667 0.0004666667 1.000000e+00 1.487047e-19   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100528 E001 957.4111624 3.491136e-03 1.483458e-60 1.519117e-57 14 54423561 54426935 3375 - 3.118 2.578 -1.800
ENSG00000100528 E002 614.8173942 1.261787e-04 3.958703e-24 3.976482e-22 14 54426936 54427328 393 - 2.740 2.846 0.353
ENSG00000100528 E003 545.3097832 2.086567e-03 1.488216e-04 7.419227e-04 14 54427329 54427424 96 - 2.710 2.758 0.160
ENSG00000100528 E004 718.3895874 9.218792e-04 2.749444e-04 1.277748e-03 14 54427425 54427522 98 - 2.841 2.862 0.070
ENSG00000100528 E005 615.8327433 3.247259e-04 2.359986e-10 3.904037e-09 14 54427523 54427594 72 - 2.759 2.818 0.196
ENSG00000100528 E006 1074.7516054 8.870328e-05 2.659482e-18 1.390262e-16 14 54427595 54427841 247 - 3.004 3.056 0.173
ENSG00000100528 E007 3.7622540 4.252857e-03 9.839007e-01 9.940115e-01 14 54430158 54430260 103 - 0.686 0.655 -0.129
ENSG00000100528 E008 978.4413740 1.022084e-04 5.678557e-06 3.991043e-05 14 54430261 54430404 144 - 2.981 2.991 0.034
ENSG00000100528 E009 813.9578513 1.309948e-03 9.702282e-01 9.853656e-01 14 54432108 54432220 113 - 2.922 2.875 -0.155
ENSG00000100528 E010 2.0002125 7.318188e-03 8.513955e-01 9.078565e-01 14 54436079 54436126 48 - 0.500 0.441 -0.299
ENSG00000100528 E011 618.4977762 1.321618e-03 3.584762e-01 5.028823e-01 14 54436369 54436437 69 - 2.810 2.743 -0.224
ENSG00000100528 E012 0.6256415 1.726718e-02 7.447796e-01 8.329787e-01 14 54436670 54436785 116 - 0.236 0.158 -0.712
ENSG00000100528 E013 507.2166716 2.859517e-03 6.646698e-02 1.374984e-01 14 54441247 54441391 145 - 2.737 2.633 -0.347