ENSG00000100522

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216410 ENSG00000100522 HEK293_OSMI2_2hA HEK293_TMG_2hB GNPNAT1 protein_coding protein_coding 27.85403 12.7124 45.78506 1.86763 2.797869 1.847821 19.122208 10.23468515 30.4828852 2.07105582 1.2144842 1.573597 0.71220417 0.79673333 0.66840000 -0.12833333 0.29298987 0.01043093 FALSE TRUE
ENST00000553987 ENSG00000100522 HEK293_OSMI2_2hA HEK293_TMG_2hB GNPNAT1 protein_coding retained_intron 27.85403 12.7124 45.78506 1.86763 2.797869 1.847821 1.313627 1.05972544 0.3995956 0.87485177 0.1998029 -1.384969 0.05940417 0.08790000 0.00860000 -0.07930000 0.75677452 0.01043093   FALSE
ENST00000557604 ENSG00000100522 HEK293_OSMI2_2hA HEK293_TMG_2hB GNPNAT1 protein_coding protein_coding 27.85403 12.7124 45.78506 1.86763 2.797869 1.847821 2.134416 0.58102955 5.0211349 0.15754476 0.9485505 3.089582 0.06780000 0.05096667 0.10803333 0.05706667 0.23460751 0.01043093 FALSE FALSE
MSTRG.9550.1 ENSG00000100522 HEK293_OSMI2_2hA HEK293_TMG_2hB GNPNAT1 protein_coding   27.85403 12.7124 45.78506 1.86763 2.797869 1.847821 1.618288 0.73953112 3.2180919 0.28265875 0.1419801 2.106621 0.05430417 0.05553333 0.07113333 0.01560000 0.71235159 0.01043093 TRUE TRUE
MSTRG.9550.7 ENSG00000100522 HEK293_OSMI2_2hA HEK293_TMG_2hB GNPNAT1 protein_coding   27.85403 12.7124 45.78506 1.86763 2.797869 1.847821 3.640214 0.07824089 6.6633497 0.07824089 1.4855922 6.240820 0.10566250 0.00770000 0.14383333 0.13613333 0.01043093 0.01043093 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100522 E001 2.029355 0.0093993575 2.151515e-02 5.467518e-02 14 52775088 52775192 105 - 0.288 0.694 2.070
ENSG00000100522 E002 421.394101 0.0188018847 8.548984e-05 4.539689e-04 14 52775193 52775687 495 - 2.477 2.773 0.988
ENSG00000100522 E003 244.305062 0.0143320719 6.506100e-02 1.351389e-01 14 52775688 52775863 176 - 2.299 2.456 0.525
ENSG00000100522 E004 105.797961 0.0039133740 3.801159e-02 8.737495e-02 14 52775864 52775946 83 - 1.951 2.083 0.442
ENSG00000100522 E005 97.613466 0.0004750685 1.707916e-03 6.306588e-03 14 52775947 52776046 100 - 1.910 2.059 0.502
ENSG00000100522 E006 319.653100 0.0004230187 2.129049e-06 1.645958e-05 14 52776047 52776556 510 - 2.426 2.560 0.447
ENSG00000100522 E007 187.274922 0.0002252948 1.106842e-01 2.065382e-01 14 52776557 52776848 292 - 2.240 2.223 -0.058
ENSG00000100522 E008 67.532903 0.0097472405 4.719900e-02 1.041914e-01 14 52776849 52776867 19 - 1.824 1.711 -0.380
ENSG00000100522 E009 836.046596 0.0008206344 2.397475e-04 1.132274e-03 14 52776868 52778374 1507 - 2.893 2.854 -0.128
ENSG00000100522 E010 200.386891 0.0073189387 1.305807e-01 2.349657e-01 14 52778375 52778458 84 - 2.280 2.213 -0.224
ENSG00000100522 E011 7.824626 0.0096621015 3.419855e-02 8.014365e-02 14 52778791 52778849 59 - 0.976 0.694 -1.102
ENSG00000100522 E012 200.677448 0.0115823605 9.184697e-02 1.780047e-01 14 52780679 52780740 62 - 2.287 2.191 -0.320
ENSG00000100522 E013 2.511928 0.1074827883 2.203350e-01 3.508140e-01 14 52781741 52781783 43 - 0.430 0.690 1.203
ENSG00000100522 E014 280.062828 0.0092470359 5.654533e-02 1.206698e-01 14 52781784 52781911 128 - 2.431 2.338 -0.310
ENSG00000100522 E015 195.632325 0.0070138270 5.532115e-02 1.185475e-01 14 52783423 52783485 63 - 2.275 2.190 -0.283
ENSG00000100522 E016 263.742582 0.0068849634 1.997241e-03 7.217108e-03 14 52784497 52784654 158 - 2.416 2.282 -0.447
ENSG00000100522 E017 103.836698 0.0018305928 2.808484e-04 1.301864e-03 14 52784655 52784664 10 - 2.016 1.876 -0.469
ENSG00000100522 E018 6.917481 0.0115573339 2.152742e-01 3.447369e-01 14 52787833 52787871 39 - 0.900 0.741 -0.623
ENSG00000100522 E019 130.621385 0.0050744510 9.575129e-04 3.813365e-03 14 52791428 52791668 241 - 2.116 1.968 -0.498