ENSG00000100519

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000445930 ENSG00000100519 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC6 protein_coding protein_coding 78.57866 23.1033 127.4664 1.556675 4.298849 2.463435 10.78989 9.0170985 16.41614 0.7633283 4.070651 0.8636589 0.21960000 0.39043333 0.13103333 -0.25940000 0.003338547 0.000481922 FALSE TRUE
ENST00000556813 ENSG00000100519 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC6 protein_coding protein_coding 78.57866 23.1033 127.4664 1.556675 4.298849 2.463435 12.28977 0.8119758 23.35732 0.6597309 1.563924 4.8292526 0.10716667 0.03263333 0.18340000 0.15076667 0.092521148 0.000481922 FALSE FALSE
ENST00000612399 ENSG00000100519 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC6 protein_coding protein_coding 78.57866 23.1033 127.4664 1.556675 4.298849 2.463435 10.05931 0.8813503 11.67435 0.5276461 5.195307 3.7124415 0.09293333 0.03560000 0.08923333 0.05363333 0.580891454 0.000481922 FALSE TRUE
MSTRG.9547.4 ENSG00000100519 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMC6 protein_coding   78.57866 23.1033 127.4664 1.556675 4.298849 2.463435 38.16758 9.7163751 63.24695 0.7259054 1.814591 2.7012499 0.45917083 0.42233333 0.49636667 0.07403333 0.212794839 0.000481922 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100519 E001 2.2843169 0.0062343247 5.288481e-01 6.617220e-01 14 52707178 52707183 6 + 0.489 0.366 -0.659
ENSG00000100519 E002 2.5873478 0.0057313923 3.942232e-01 5.380875e-01 14 52707184 52707191 8 + 0.532 0.366 -0.865
ENSG00000100519 E003 11.3247012 0.0016516050 1.625199e-02 4.327932e-02 14 52707192 52707199 8 + 1.076 0.750 -1.239
ENSG00000100519 E004 12.5250695 0.0013564975 1.434182e-02 3.900608e-02 14 52707200 52707200 1 + 1.115 0.798 -1.187
ENSG00000100519 E005 14.6016563 0.0011755068 3.488185e-02 8.142566e-02 14 52707201 52707201 1 + 1.165 0.917 -0.908
ENSG00000100519 E006 25.4096707 0.0007391076 1.770523e-02 4.649897e-02 14 52707202 52707208 7 + 1.388 1.172 -0.756
ENSG00000100519 E007 68.4395148 0.0003613734 7.332981e-07 6.271268e-06 14 52707209 52707218 10 + 1.817 1.523 -0.999
ENSG00000100519 E008 74.3892909 0.0003361786 4.170068e-07 3.747483e-06 14 52707219 52707221 3 + 1.852 1.564 -0.975
ENSG00000100519 E009 150.4290358 0.0002835995 7.992298e-16 3.055735e-14 14 52707222 52707230 9 + 2.161 1.826 -1.122
ENSG00000100519 E010 285.7518506 0.0001710188 9.401343e-11 1.666277e-09 14 52707231 52707286 56 + 2.416 2.236 -0.601
ENSG00000100519 E011 284.0692137 0.0003341456 2.914791e-09 3.965233e-08 14 52707287 52707304 18 + 2.412 2.242 -0.565
ENSG00000100519 E012 7.2364344 0.0022273420 1.983202e-01 3.241185e-01 14 52707305 52707529 225 + 0.889 0.696 -0.766
ENSG00000100519 E013 402.6802970 0.0001252416 2.270815e-06 1.745088e-05 14 52708309 52708388 80 + 2.551 2.446 -0.353
ENSG00000100519 E014 310.7792738 0.0001554373 5.480404e-05 3.057352e-04 14 52708483 52708522 40 + 2.439 2.336 -0.344
ENSG00000100519 E015 1.1792638 0.0119314319 6.065245e-01 7.266534e-01 14 52708523 52708706 184 + 0.327 0.220 -0.767
ENSG00000100519 E016 0.7427016 0.0153787590 3.112822e-01 4.537280e-01 14 52708707 52708763 57 + 0.256 0.000 -10.320
ENSG00000100519 E017 338.0168568 0.0004503643 4.321999e-02 9.695441e-02 14 52708764 52708816 53 + 2.466 2.414 -0.170
ENSG00000100519 E018 3.7554639 0.0307146112 1.333756e-01 2.388153e-01 14 52708817 52709089 273 + 0.672 0.365 -1.486
ENSG00000100519 E019 20.5562875 0.0200817715 2.373724e-01 3.711315e-01 14 52709575 52710335 761 + 1.227 1.348 0.424
ENSG00000100519 E020 22.2214813 0.0017428681 4.860084e-02 1.066831e-01 14 52710546 52711100 555 + 1.251 1.416 0.575
ENSG00000100519 E021 383.1491697 0.0003786880 4.799410e-03 1.537260e-02 14 52711101 52711168 68 + 2.523 2.456 -0.224
ENSG00000100519 E022 491.8148744 0.0001411699 2.084212e-03 7.484839e-03 14 52711410 52711524 115 + 2.628 2.567 -0.205
ENSG00000100519 E023 346.3355517 0.0006515925 2.248111e-01 3.562032e-01 14 52713881 52713968 88 + 2.471 2.438 -0.110
ENSG00000100519 E024 0.1451727 0.0432250910 1.000000e+00   14 52713969 52714032 64 + 0.065 0.000 -8.050
ENSG00000100519 E025 336.6392596 0.0002083018 5.296335e-01 6.623844e-01 14 52718081 52718142 62 + 2.454 2.438 -0.052
ENSG00000100519 E026 3.8735562 0.0429430700 2.927447e-03 1.005122e-02 14 52718143 52718228 86 + 0.714 0.000 -12.686
ENSG00000100519 E027 133.7174482 0.0002535497 4.917993e-01 6.290625e-01 14 52718229 52718230 2 + 2.046 2.071 0.085
ENSG00000100519 E028 161.8704153 0.0002270458 5.192050e-01 6.533327e-01 14 52718231 52718239 9 + 2.130 2.151 0.072
ENSG00000100519 E029 337.8105442 0.0003928786 1.125845e-02 3.178184e-02 14 52718240 52718352 113 + 2.440 2.502 0.205
ENSG00000100519 E030 2.0777472 0.0081748121 1.959702e-01 3.211701e-01 14 52718353 52718506 154 + 0.489 0.220 -1.660
ENSG00000100519 E031 6.3603021 0.0025515870 1.891531e-01 3.126701e-01 14 52718507 52718976 470 + 0.841 0.634 -0.846
ENSG00000100519 E032 330.1990815 0.0002692713 4.686751e-02 1.035937e-01 14 52718977 52719038 62 + 2.434 2.482 0.159
ENSG00000100519 E033 431.3362830 0.0001482571 4.547914e-07 4.055107e-06 14 52720861 52720981 121 + 2.536 2.636 0.334
ENSG00000100519 E034 393.6035406 0.0001246491 2.060556e-10 3.443294e-09 14 52721110 52721190 81 + 2.487 2.617 0.433
ENSG00000100519 E035 43.8192027 0.0029539476 2.803452e-04 1.299722e-03 14 52721500 52723744 2245 + 1.626 1.345 -0.964
ENSG00000100519 E036 10.6228897 0.0017937386 5.195686e-03 1.646246e-02 14 52723745 52723874 130 + 0.906 1.209 1.105
ENSG00000100519 E037 7.2512016 0.0025153608 3.148343e-02 7.485037e-02 14 52723875 52723964 90 + 0.764 1.038 1.042
ENSG00000100519 E038 363.2334452 0.0001365173 1.775784e-13 4.878436e-12 14 52723965 52724036 72 + 2.445 2.601 0.519
ENSG00000100519 E039 413.9040032 0.0001561211 1.351261e-45 6.513798e-43 14 52727499 52728590 1092 + 2.464 2.736 0.905