ENSG00000100425

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000404034 ENSG00000100425 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD1 protein_coding protein_coding 14.93618 14.91576 14.64481 0.6209757 0.2340521 -0.02643015 5.3778486 4.4724262 5.4306174 0.11734962 0.3286706 0.27949056 0.35520417 0.30120000 0.37080000 0.06960000 0.2768480455 5.20721e-05 FALSE TRUE
ENST00000404760 ENSG00000100425 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD1 protein_coding protein_coding 14.93618 14.91576 14.64481 0.6209757 0.2340521 -0.02643015 4.2751056 4.5112562 4.6778657 0.40646262 0.5261922 0.05220755 0.28664583 0.30123333 0.31886667 0.01763333 0.9000788640 5.20721e-05 FALSE TRUE
ENST00000438393 ENSG00000100425 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD1 protein_coding nonsense_mediated_decay 14.93618 14.91576 14.64481 0.6209757 0.2340521 -0.02643015 1.6674522 3.8489747 0.4486086 0.17984191 0.4486086 -3.07288220 0.11532917 0.25826667 0.03143333 -0.22683333 0.0473563234 5.20721e-05 TRUE TRUE
MSTRG.22349.10 ENSG00000100425 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD1 protein_coding   14.93618 14.91576 14.64481 0.6209757 0.2340521 -0.02643015 0.9552913 0.7349937 0.8866234 0.03714990 0.1733076 0.26727390 0.06371250 0.04963333 0.06086667 0.01123333 0.8337268239 5.20721e-05 TRUE TRUE
MSTRG.22349.8 ENSG00000100425 HEK293_OSMI2_2hA HEK293_TMG_2hB BRD1 protein_coding   14.93618 14.91576 14.64481 0.6209757 0.2340521 -0.02643015 1.0050787 0.4543650 1.6400091 0.08657089 0.2016690 1.82914274 0.06770417 0.03016667 0.11190000 0.08173333 0.0000520721 5.20721e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100425 E001 1.0714635 0.3713827453 7.241206e-02 1.473544e-01 22 49773283 49773292 10 - 0.000 0.456 11.157
ENSG00000100425 E002 88.2805131 0.0048329569 1.649939e-13 4.551858e-12 22 49773293 49773439 147 - 1.674 2.053 1.277
ENSG00000100425 E003 245.7928165 0.0016537511 1.640621e-16 6.874824e-15 22 49773440 49773708 269 - 2.226 2.456 0.767
ENSG00000100425 E004 212.0404401 0.0002003174 2.442916e-11 4.779050e-10 22 49773709 49773968 260 - 2.217 2.368 0.502
ENSG00000100425 E005 115.8069909 0.0014105604 6.388420e-06 4.440206e-05 22 49773969 49774082 114 - 1.954 2.108 0.515
ENSG00000100425 E006 239.1455566 0.0006981259 9.231538e-01 9.555455e-01 22 49774083 49774416 334 - 2.379 2.359 -0.065
ENSG00000100425 E007 4.6532290 0.0117161891 7.237037e-01 8.174595e-01 22 49775261 49775590 330 - 0.707 0.745 0.154
ENSG00000100425 E008 162.9174575 0.0033923250 1.863017e-01 3.091034e-01 22 49775591 49775745 155 - 2.243 2.176 -0.224
ENSG00000100425 E009 128.7632600 0.0041611287 5.392616e-01 6.705490e-01 22 49776050 49776159 110 - 2.096 2.106 0.034
ENSG00000100425 E010 137.9781468 0.0010871005 1.647771e-01 2.814049e-01 22 49777034 49777161 128 - 2.112 2.142 0.098
ENSG00000100425 E011 144.7764447 0.0002903040 5.557611e-01 6.845816e-01 22 49777678 49777813 136 - 2.150 2.150 0.001
ENSG00000100425 E012 19.8617564 0.0105434683 6.784562e-02 1.397932e-01 22 49777814 49778103 290 - 1.212 1.364 0.533
ENSG00000100425 E013 105.1328403 0.0022421648 9.946071e-01 1.000000e+00 22 49787390 49787494 105 - 2.021 2.004 -0.057
ENSG00000100425 E014 33.9381960 0.0019931360 9.528219e-01 9.744510e-01 22 49787495 49787522 28 - 1.538 1.517 -0.072
ENSG00000100425 E015 43.0393323 0.0277198434 3.490681e-01 4.933006e-01 22 49787523 49787576 54 - 1.574 1.658 0.286
ENSG00000100425 E016 86.2694021 0.0003135442 3.397881e-01 4.837115e-01 22 49787577 49787887 311 - 1.915 1.935 0.065
ENSG00000100425 E017 160.7171656 0.0002772548 6.157083e-02 1.292754e-01 22 49794034 49794294 261 - 2.237 2.168 -0.233
ENSG00000100425 E018 170.5966750 0.0002115154 5.339885e-03 1.685705e-02 22 49797805 49798117 313 - 2.275 2.183 -0.309
ENSG00000100425 E019 87.8371256 0.0005998972 2.025574e-03 7.306249e-03 22 49798558 49798680 123 - 2.015 1.879 -0.455
ENSG00000100425 E020 30.1587806 0.0079164531 1.154654e-01 2.135251e-01 22 49798681 49798686 6 - 1.557 1.421 -0.465
ENSG00000100425 E021 90.7010391 0.0003194975 4.048319e-01 5.485170e-01 22 49798988 49799119 132 - 1.977 1.929 -0.163
ENSG00000100425 E022 107.8704386 0.0144150620 3.797683e-01 5.240202e-01 22 49804204 49804360 157 - 2.066 1.993 -0.243
ENSG00000100425 E023 0.9975554 0.0172951034 2.591277e-01 3.964974e-01 22 49818212 49818383 172 - 0.138 0.331 1.615
ENSG00000100425 E024 1.2470141 0.0108647759 3.969550e-01 5.407294e-01 22 49820833 49820986 154 - 0.242 0.384 0.933
ENSG00000100425 E025 469.8810619 0.0032168138 1.545656e-12 3.670343e-11 22 49822951 49824331 1381 - 2.778 2.565 -0.709
ENSG00000100425 E026 1.4434697 0.0591368311 2.171346e-01 3.469355e-01 22 49824332 49824804 473 - 0.510 0.268 -1.389
ENSG00000100425 E027 4.3671723 0.0037729783 1.418243e-04 7.113634e-04 22 49825730 49825900 171 - 0.981 0.432 -2.331
ENSG00000100425 E028 14.5879572 0.0208291955 7.864036e-09 9.883363e-08 22 49827497 49827873 377 - 1.472 0.787 -2.485