ENSG00000100413

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000337566 ENSG00000100413 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3H protein_coding protein_coding 104.4041 181.2618 73.24668 2.147001 0.8334365 -1.307123 23.821465 45.770683 13.056062 1.1912286 1.1787766 -1.80891463 0.21114167 0.25260000 0.17853333 -0.07406667 5.005102e-02 6.813009e-20 FALSE TRUE
ENST00000355209 ENSG00000100413 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3H protein_coding protein_coding 104.4041 181.2618 73.24668 2.147001 0.8334365 -1.307123 11.562796 17.177352 16.223323 3.6141299 1.3228501 -0.08238893 0.11621250 0.09446667 0.22183333 0.12736667 1.261585e-02 6.813009e-20 FALSE TRUE
ENST00000396504 ENSG00000100413 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3H protein_coding protein_coding 104.4041 181.2618 73.24668 2.147001 0.8334365 -1.307123 26.926913 49.755106 14.951919 2.7336517 1.7523953 -1.73383926 0.25380000 0.27486667 0.20383333 -0.07103333 1.971175e-01 6.813009e-20 FALSE TRUE
ENST00000407461 ENSG00000100413 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3H protein_coding protein_coding 104.4041 181.2618 73.24668 2.147001 0.8334365 -1.307123 9.419414 9.311911 6.378674 4.6884794 1.1745589 -0.54510932 0.09886667 0.05086667 0.08713333 0.03626667 5.231297e-01 6.813009e-20 FALSE TRUE
ENST00000431534 ENSG00000100413 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3H protein_coding nonsense_mediated_decay 104.4041 181.2618 73.24668 2.147001 0.8334365 -1.307123 19.366165 31.603337 14.185793 0.2943621 0.6988628 -1.15506987 0.19078333 0.17436667 0.19350000 0.01913333 4.121133e-01 6.813009e-20 FALSE TRUE
MSTRG.22207.8 ENSG00000100413 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3H protein_coding   104.4041 181.2618 73.24668 2.147001 0.8334365 -1.307123 7.075171 21.144668 0.000000 4.8274422 0.0000000 -11.04676032 0.06027500 0.11720000 0.00000000 -0.11720000 6.813009e-20 6.813009e-20 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100413 E001 10.570841 1.686882e-03 1.336299e-01 2.391930e-01 22 41525799 41525803 5 - 1.119 0.955 -0.601
ENSG00000100413 E002 4038.633921 4.882928e-04 1.682045e-02 4.453585e-02 22 41525804 41528841 3038 - 3.557 3.530 -0.091
ENSG00000100413 E003 158.661888 9.680910e-04 6.518872e-07 5.634807e-06 22 41528842 41528842 1 - 2.256 2.085 -0.571
ENSG00000100413 E004 254.534613 2.815152e-04 1.510663e-04 7.517679e-04 22 41528843 41528864 22 - 2.411 2.311 -0.335
ENSG00000100413 E005 1825.729134 2.477409e-04 4.656996e-03 1.497732e-02 22 41528865 41529211 347 - 3.171 3.199 0.093
ENSG00000100413 E006 1129.293288 4.174409e-04 6.474262e-08 6.821253e-07 22 41529212 41529301 90 - 2.923 3.002 0.262
ENSG00000100413 E007 636.176016 1.042095e-03 3.029987e-02 7.255957e-02 22 41529302 41529304 3 - 2.702 2.747 0.150
ENSG00000100413 E008 723.458889 9.332760e-04 2.111796e-03 7.570981e-03 22 41529305 41529336 32 - 2.745 2.806 0.203
ENSG00000100413 E009 1557.778353 7.475215e-05 9.682645e-04 3.849952e-03 22 41530687 41530888 202 - 3.101 3.131 0.101
ENSG00000100413 E010 699.748159 1.033057e-04 8.996981e-01 9.400134e-01 22 41532094 41532118 25 - 2.778 2.775 -0.008
ENSG00000100413 E011 723.395025 1.161981e-04 4.371215e-01 5.791449e-01 22 41532119 41532157 39 - 2.803 2.787 -0.054
ENSG00000100413 E012 59.601177 1.057890e-03 1.573099e-01 2.715285e-01 22 41532604 41532624 21 - 1.768 1.690 -0.265
ENSG00000100413 E013 62.389037 4.052107e-03 2.153647e-01 3.448525e-01 22 41532625 41532658 34 - 1.787 1.711 -0.257
ENSG00000100413 E014 802.382486 1.172822e-04 8.460388e-01 9.042771e-01 22 41532659 41532745 87 - 2.837 2.835 -0.005
ENSG00000100413 E015 16.096875 1.153822e-03 2.068203e-01 3.347414e-01 22 41533522 41533687 166 - 1.253 1.134 -0.422
ENSG00000100413 E016 13.422891 2.672945e-02 2.632371e-01 4.011154e-01 22 41539143 41540698 1556 - 1.198 1.062 -0.489
ENSG00000100413 E017 811.960882 3.555282e-04 3.234064e-02 7.651374e-02 22 41540699 41540795 97 - 2.870 2.831 -0.130
ENSG00000100413 E018 2.795928 5.533857e-03 2.382060e-01 3.720577e-01 22 41543168 41543239 72 - 0.672 0.478 -0.885
ENSG00000100413 E019 1.553168 9.876089e-03 6.237901e-03 1.923737e-02 22 41543368 41543430 63 - 0.672 0.204 -2.623
ENSG00000100413 E020 10.505832 1.510133e-02 2.448325e-02 6.082030e-02 22 41543752 41543990 239 - 1.184 0.915 -0.980
ENSG00000100413 E021 836.540554 1.786250e-03 2.672428e-01 4.055361e-01 22 41543991 41544121 131 - 2.877 2.845 -0.105
ENSG00000100413 E022 652.079122 2.210677e-03 1.580775e-01 2.725604e-01 22 41544122 41544395 274 - 2.777 2.734 -0.144
ENSG00000100413 E023 24.820540 5.355104e-02 7.452420e-05 4.018897e-04 22 41544396 41544427 32 - 1.673 1.146 -1.827
ENSG00000100413 E024 50.115380 1.460920e-02 4.119541e-01 5.555091e-01 22 41544428 41544489 62 - 1.691 1.618 -0.248
ENSG00000100413 E025 1.568606 2.369292e-01 7.751647e-01 8.551268e-01 22 41544511 41544606 96 - 0.313 0.383 0.424