ENSG00000100393

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263253 ENSG00000100393 HEK293_OSMI2_2hA HEK293_TMG_2hB EP300 protein_coding protein_coding 16.85869 16.47963 16.63601 1.83613 0.2944009 0.01361704 8.369739 3.584132 11.5548893 0.5960069 0.34808093 1.686036 0.492250 0.2189000 0.69426667 0.47536667 2.705264e-15 4.520754e-16 FALSE TRUE
ENST00000635083 ENSG00000100393 HEK293_OSMI2_2hA HEK293_TMG_2hB EP300 protein_coding retained_intron 16.85869 16.47963 16.63601 1.83613 0.2944009 0.01361704 3.624601 8.815560 0.2966179 1.6053855 0.07440269 -4.847174 0.219725 0.5358333 0.01773333 -0.51810000 4.520754e-16 4.520754e-16 FALSE TRUE
ENST00000674155 ENSG00000100393 HEK293_OSMI2_2hA HEK293_TMG_2hB EP300 protein_coding protein_coding 16.85869 16.47963 16.63601 1.83613 0.2944009 0.01361704 4.452818 3.801454 4.3045091 0.8465638 0.19628544 0.178855 0.262150 0.2277667 0.25930000 0.03153333 7.878416e-01 4.520754e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100393 E001 41.5496360 0.0005270696 5.771462e-09 7.452196e-08 22 41092592 41093004 413 + 1.683 1.456 -0.775
ENSG00000100393 E002 36.8060752 0.0005795883 3.624821e-11 6.880187e-10 22 41093005 41093098 94 + 1.653 1.345 -1.056
ENSG00000100393 E003 0.0000000       22 41108025 41108098 74 +      
ENSG00000100393 E004 185.8566038 0.0001995513 2.206041e-43 9.349618e-41 22 41117187 41117770 584 + 2.341 2.063 -0.931
ENSG00000100393 E005 55.9923184 0.0004417639 2.020833e-09 2.825130e-08 22 41117771 41117821 51 + 1.802 1.618 -0.625
ENSG00000100393 E006 0.2944980 0.3537000674 4.368048e-01   22 41125852 41125863 12 + 0.164 0.000 -14.742
ENSG00000100393 E007 80.5829749 0.0003841576 3.080182e-14 9.428523e-13 22 41125864 41126040 177 + 1.963 1.757 -0.694
ENSG00000100393 E008 0.2955422 0.0311591260 7.618874e-01   22 41126041 41126308 268 + 0.090 0.164 0.995
ENSG00000100393 E009 96.0086547 0.0153136826 2.009386e-05 1.245696e-04 22 41127487 41127748 262 + 2.030 1.856 -0.583
ENSG00000100393 E010 69.8472910 0.0021737400 9.682114e-11 1.712170e-09 22 41129890 41130003 114 + 1.901 1.694 -0.699
ENSG00000100393 E011 73.6249784 0.0008720239 9.505613e-10 1.415063e-08 22 41131388 41131532 145 + 1.909 1.750 -0.538
ENSG00000100393 E012 66.7914285 0.0004221174 2.224094e-07 2.113503e-06 22 41131533 41131633 101 + 1.854 1.735 -0.401
ENSG00000100393 E013 61.2140454 0.0030523243 2.571739e-07 2.412400e-06 22 41135813 41135906 94 + 1.831 1.669 -0.549
ENSG00000100393 E014 95.1956050 0.0149691321 1.570370e-05 9.975918e-05 22 41137653 41137790 138 + 2.026 1.851 -0.585
ENSG00000100393 E015 94.5730543 0.0114603985 1.771421e-05 1.111705e-04 22 41140140 41140257 118 + 2.014 1.868 -0.491
ENSG00000100393 E016 101.7346820 0.0236621809 1.105779e-03 4.322341e-03 22 41141048 41141222 175 + 2.041 1.913 -0.430
ENSG00000100393 E017 0.2944980 0.3537000674 4.368048e-01   22 41146553 41146738 186 + 0.164 0.000 -14.742
ENSG00000100393 E018 63.5031590 0.0035423377 3.623218e-07 3.295601e-06 22 41146739 41146816 78 + 1.849 1.689 -0.538
ENSG00000100393 E019 78.8387736 0.0046844103 2.572529e-04 1.205496e-03 22 41147837 41147946 110 + 1.912 1.841 -0.239
ENSG00000100393 E020 0.3299976 0.0258485360 1.100576e-01   22 41148819 41149037 219 + 0.000 0.283 16.459
ENSG00000100393 E021 86.4318915 0.0004392642 4.914652e-04 2.127908e-03 22 41149038 41149175 138 + 1.931 1.911 -0.066
ENSG00000100393 E022 155.8952869 0.0002359358 4.980888e-05 2.808376e-04 22 41149761 41150198 438 + 2.178 2.178 0.000
ENSG00000100393 E023 113.4209945 0.0002995815 9.998544e-07 8.294772e-06 22 41151833 41152012 180 + 2.057 2.006 -0.173
ENSG00000100393 E024 109.1892325 0.0003020838 1.199753e-03 4.639510e-03 22 41152206 41152350 145 + 2.020 2.025 0.018
ENSG00000100393 E025 115.6185989 0.0072095028 1.513714e-01 2.635328e-01 22 41154995 41155113 119 + 2.029 2.082 0.178
ENSG00000100393 E026 72.9278759 0.0045673461 6.797863e-03 2.069775e-02 22 41157169 41157199 31 + 1.858 1.837 -0.073
ENSG00000100393 E027 147.0467023 0.0048044224 2.496196e-03 8.751999e-03 22 41157200 41157408 209 + 2.157 2.148 -0.030
ENSG00000100393 E028 77.0994691 0.0004052815 1.940442e-01 3.188642e-01 22 41158412 41158467 56 + 1.849 1.914 0.219
ENSG00000100393 E029 74.6038048 0.0088423971 3.652640e-01 5.095935e-01 22 41158468 41158500 33 + 1.836 1.906 0.239
ENSG00000100393 E030 7.7845226 0.0123699249 2.632333e-01 4.011129e-01 22 41158501 41160419 1919 + 0.935 0.899 -0.134
ENSG00000100393 E031 99.3282030 0.0004597162 9.511409e-01 9.734412e-01 22 41160642 41160722 81 + 1.934 2.056 0.408
ENSG00000100393 E032 54.2699096 0.0008615690 2.326916e-01 3.656489e-01 22 41162723 41162726 4 + 1.651 1.828 0.598
ENSG00000100393 E033 88.6235574 0.0003612428 9.041716e-01 9.430052e-01 22 41162727 41162779 53 + 1.884 2.008 0.415
ENSG00000100393 E034 105.3784152 0.0003761879 3.247511e-01 4.681697e-01 22 41164053 41164130 78 + 1.973 2.058 0.283
ENSG00000100393 E035 90.1996322 0.0023129342 1.844591e-01 3.068115e-01 22 41166599 41166666 68 + 1.918 1.981 0.210
ENSG00000100393 E036 130.8164276 0.0002742531 2.383483e-01 3.722323e-01 22 41168449 41168599 151 + 2.071 2.153 0.274
ENSG00000100393 E037 123.5781116 0.0002380374 1.366104e-01 2.433493e-01 22 41168721 41168867 147 + 2.050 2.121 0.237
ENSG00000100393 E038 0.7363589 0.0154959969 4.873984e-02 1.069287e-01 22 41169283 41169502 220 + 0.333 0.000 -16.064
ENSG00000100393 E039 109.5727865 0.0059152685 7.491697e-02 1.513979e-01 22 41169503 41169616 114 + 2.015 2.046 0.103
ENSG00000100393 E040 151.8898042 0.0055232709 3.735323e-01 5.179626e-01 22 41170406 41170571 166 + 2.134 2.217 0.277
ENSG00000100393 E041 161.1608521 0.0015566908 6.160734e-01 7.342073e-01 22 41172499 41172663 165 + 2.133 2.272 0.466
ENSG00000100393 E042 150.2839180 0.0020858585 2.732423e-02 6.665413e-02 22 41173623 41173784 162 + 2.072 2.275 0.680
ENSG00000100393 E043 4.9325206 0.0075739702 4.958484e-02 1.084260e-01 22 41176059 41176246 188 + 0.815 0.626 -0.779
ENSG00000100393 E044 182.2306089 0.0073635862 8.001894e-02 1.595906e-01 22 41176247 41176528 282 + 2.149 2.368 0.733
ENSG00000100393 E045 1459.4129567 0.0173267564 4.233300e-11 7.952266e-10 22 41176773 41180077 3305 + 2.929 3.371 1.468