ENSG00000100354

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335727 ENSG00000100354 HEK293_OSMI2_2hA HEK293_TMG_2hB TNRC6B protein_coding protein_coding 3.900031 2.708333 4.436982 0.2348218 0.6222369 0.7101045 1.59667314 1.4655474 1.4830432 0.05867578 0.23521405 0.01700565 0.41827500 0.5462333 0.33283333 -0.21340000 0.001835862 0.001835862 FALSE TRUE
ENST00000402203 ENSG00000100354 HEK293_OSMI2_2hA HEK293_TMG_2hB TNRC6B protein_coding protein_coding 3.900031 2.708333 4.436982 0.2348218 0.6222369 0.7101045 0.42347500 0.6709232 0.2764670 0.07161095 0.14385935 -1.24912299 0.11791667 0.2469000 0.05713333 -0.18976667 0.087226732 0.001835862 FALSE TRUE
ENST00000441751 ENSG00000100354 HEK293_OSMI2_2hA HEK293_TMG_2hB TNRC6B protein_coding protein_coding 3.900031 2.708333 4.436982 0.2348218 0.6222369 0.7101045 0.06374208 0.0000000 0.5099367 0.00000000 0.50993667 5.70026399 0.01125833 0.0000000 0.09006667 0.09006667 0.883986832 0.001835862   FALSE
ENST00000446273 ENSG00000100354 HEK293_OSMI2_2hA HEK293_TMG_2hB TNRC6B protein_coding protein_coding 3.900031 2.708333 4.436982 0.2348218 0.6222369 0.7101045 0.94564110 0.3345867 1.0765766 0.15590424 0.05837943 1.65685091 0.22602500 0.1153333 0.25243333 0.13710000 0.227273179 0.001835862 FALSE TRUE
ENST00000489500 ENSG00000100354 HEK293_OSMI2_2hA HEK293_TMG_2hB TNRC6B protein_coding retained_intron 3.900031 2.708333 4.436982 0.2348218 0.6222369 0.7101045 0.17781904 0.0000000 0.3888380 0.00000000 0.20196254 5.31773113 0.03540000 0.0000000 0.08046667 0.08046667 0.225742911 0.001835862   FALSE
MSTRG.22186.1 ENSG00000100354 HEK293_OSMI2_2hA HEK293_TMG_2hB TNRC6B protein_coding   3.900031 2.708333 4.436982 0.2348218 0.6222369 0.7101045 0.33124518 0.0000000 0.4719132 0.00000000 0.24364734 5.59070152 0.08310833 0.0000000 0.12460000 0.12460000 0.290622312 0.001835862 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100354 E001 0.5880631 0.4001878917 6.091707e-01 0.7287038382 22 40044817 40044840 24 + 0.118 0.233 1.179
ENSG00000100354 E002 2.2875366 0.0366159061 1.723468e-01 0.2912551398 22 40044841 40044998 158 + 0.616 0.373 -1.206
ENSG00000100354 E003 0.4439371 0.0215560643 9.653513e-02 0.1851641297 22 40045474 40045588 115 + 0.288 0.000 -10.503
ENSG00000100354 E004 3.6055297 0.0046116610 9.859601e-03 0.0284186370 22 40117055 40117128 74 + 0.822 0.430 -1.739
ENSG00000100354 E005 2.2068970 0.0255719784 5.066376e-02 0.1103389093 22 40125772 40125773 2 + 0.648 0.304 -1.763
ENSG00000100354 E006 5.6905632 0.0029283876 2.545174e-04 0.0011940359 22 40125774 40125862 89 + 1.017 0.527 -1.992
ENSG00000100354 E007 5.1693094 0.0033414892 4.778515e-05 0.0002705383 22 40156115 40156182 68 + 1.004 0.430 -2.428
ENSG00000100354 E008 1.2062186 0.1328340764 6.644200e-02 0.1374602711 22 40177925 40177941 17 + 0.504 0.127 -2.694
ENSG00000100354 E009 2.0166849 0.0686186121 2.623217e-01 0.4001034602 22 40177942 40177948 7 + 0.582 0.371 -1.063
ENSG00000100354 E010 13.5348011 0.0069418644 4.925388e-01 0.6296944054 22 40177949 40178140 192 + 1.197 1.130 -0.237
ENSG00000100354 E011 17.4024900 0.0010699511 3.501950e-02 0.0816916044 22 40246015 40246102 88 + 1.343 1.172 -0.601
ENSG00000100354 E012 0.0000000       22 40246103 40246362 260 +      
ENSG00000100354 E013 8.3594784 0.0024735040 1.450557e-03 0.0054753747 22 40251179 40251200 22 + 1.121 0.758 -1.370
ENSG00000100354 E014 22.8387564 0.0008289606 1.614649e-02 0.0430522270 22 40261832 40262173 342 + 1.456 1.284 -0.597
ENSG00000100354 E015 16.8726914 0.0013798924 8.624867e-03 0.0253487524 22 40264688 40264797 110 + 1.349 1.130 -0.770
ENSG00000100354 E016 29.6839943 0.0148449746 3.078734e-01 0.4501048686 22 40264798 40265002 205 + 1.527 1.440 -0.298
ENSG00000100354 E017 156.8655502 0.0192046333 1.192567e-02 0.0333552322 22 40265003 40266800 1798 + 2.276 2.117 -0.533
ENSG00000100354 E018 26.7146298 0.0007670992 4.423489e-01 0.5839716085 22 40266801 40267036 236 + 1.470 1.420 -0.172
ENSG00000100354 E019 9.0552390 0.0018872630 3.123234e-02 0.0743512565 22 40270122 40270280 159 + 0.862 1.096 0.873
ENSG00000100354 E020 29.1847448 0.0007338327 8.555990e-01 0.9107239015 22 40273425 40273600 176 + 1.484 1.473 -0.037
ENSG00000100354 E021 0.0000000       22 40276840 40276981 142 +      
ENSG00000100354 E022 24.2208319 0.0026284686 6.739633e-01 0.7795225286 22 40277077 40277151 75 + 1.411 1.380 -0.107
ENSG00000100354 E023 18.8128436 0.0009661723 9.849112e-01 0.9946027553 22 40277999 40278044 46 + 1.297 1.299 0.008
ENSG00000100354 E024 28.6600461 0.0007819586 9.767027e-01 0.9894516715 22 40279995 40280143 149 + 1.475 1.478 0.010
ENSG00000100354 E025 0.7780993 0.0277530162 1.550057e-01 0.2684245012 22 40281119 40281289 171 + 0.118 0.370 2.101
ENSG00000100354 E026 24.3742827 0.0043957880 5.262161e-01 0.6594697603 22 40285645 40285770 126 + 1.378 1.426 0.166
ENSG00000100354 E027 22.4532649 0.0051103017 5.140642e-01 0.6488116203 22 40300455 40300586 132 + 1.349 1.402 0.184
ENSG00000100354 E028 0.0000000       22 40300856 40300909 54 +      
ENSG00000100354 E029 13.4364876 0.0013782428 1.028212e-01 0.1947900984 22 40300910 40301005 96 + 1.078 1.227 0.533
ENSG00000100354 E030 25.9692971 0.0080472470 2.314532e-01 0.3642116086 22 40301150 40301333 184 + 1.372 1.465 0.321
ENSG00000100354 E031 0.0000000       22 40301334 40301495 162 +      
ENSG00000100354 E032 27.8403635 0.0042641721 8.696161e-01 0.9201586193 22 40308512 40308649 138 + 1.461 1.449 -0.043
ENSG00000100354 E033 35.5345435 0.0005706117 7.871404e-01 0.8636707442 22 40310817 40310993 177 + 1.572 1.558 -0.050
ENSG00000100354 E034 32.9668882 0.0007713962 3.209990e-01 0.4641840650 22 40312505 40312651 147 + 1.501 1.562 0.207
ENSG00000100354 E035 30.4427467 0.0075765097 9.305452e-01 0.9603170675 22 40312902 40312997 96 + 1.497 1.504 0.026
ENSG00000100354 E036 46.6681840 0.0005626258 6.263984e-01 0.7423558157 22 40315283 40315507 225 + 1.670 1.696 0.087
ENSG00000100354 E037 27.4766190 0.0007601725 9.480070e-01 0.9713305940 22 40315942 40316012 71 + 1.461 1.458 -0.011
ENSG00000100354 E038 35.5775311 0.0009022676 4.751695e-01 0.6139097747 22 40321090 40321229 140 + 1.546 1.589 0.145
ENSG00000100354 E039 0.9911135 0.0136968632 9.287784e-01 0.9592030564 22 40321230 40321799 570 + 0.288 0.304 0.108
ENSG00000100354 E040 35.8148305 0.0033583729 5.524208e-04 0.0023559152 22 40322854 40323038 185 + 1.442 1.663 0.758
ENSG00000100354 E041 38.5895020 0.0006826090 1.229619e-04 0.0006274681 22 40323039 40323348 310 + 1.475 1.693 0.743
ENSG00000100354 E042 11.0565895 0.0015451450 5.917447e-01 0.7144402857 22 40323349 40323375 27 + 1.111 1.060 -0.186
ENSG00000100354 E043 580.9613033 0.0046393154 3.387891e-02 0.0795145753 22 40323376 40335808 12433 + 2.738 2.786 0.157