ENSG00000100350

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397223 ENSG00000100350 HEK293_OSMI2_2hA HEK293_TMG_2hB FOXRED2 protein_coding protein_coding 90.2821 105.126 101.7242 8.017635 1.271506 -0.04745128 3.728797 0.00000 7.208745 0.000000 1.1735131 9.4956043 0.04030417 0.0000000 0.0706000 0.07060000 5.804886e-32 5.804886e-32 FALSE TRUE
ENST00000397224 ENSG00000100350 HEK293_OSMI2_2hA HEK293_TMG_2hB FOXRED2 protein_coding protein_coding 90.2821 105.126 101.7242 8.017635 1.271506 -0.04745128 46.697107 70.06678 38.536043 1.275379 1.3632490 -0.8623537 0.52160833 0.6729667 0.3792000 -0.29376667 3.880615e-05 5.804886e-32 FALSE TRUE
ENST00000684868 ENSG00000100350 HEK293_OSMI2_2hA HEK293_TMG_2hB FOXRED2 protein_coding protein_coding 90.2821 105.126 101.7242 8.017635 1.271506 -0.04745128 16.377094 15.63390 21.183779 2.384162 0.8766058 0.4380407 0.17940833 0.1468667 0.2081333 0.06126667 2.881149e-02 5.804886e-32 FALSE TRUE
ENST00000691242 ENSG00000100350 HEK293_OSMI2_2hA HEK293_TMG_2hB FOXRED2 protein_coding protein_coding 90.2821 105.126 101.7242 8.017635 1.271506 -0.04745128 17.115753 14.08542 25.914803 3.588527 0.2266983 0.8791066 0.18782500 0.1301000 0.2548667 0.12476667 2.384459e-02 5.804886e-32 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100350 E001 183.339906 1.891660e-02 7.093831e-08 7.411786e-07 22 36487190 36487221 32 - 1.971 2.383 1.375
ENSG00000100350 E002 3133.760024 1.193297e-02 2.601502e-08 2.965919e-07 22 36487222 36488241 1020 - 3.310 3.577 0.889
ENSG00000100350 E003 318.437110 2.223694e-03 5.798889e-04 2.459543e-03 22 36488242 36488268 27 - 2.429 2.535 0.354
ENSG00000100350 E004 234.379443 1.506085e-03 6.922461e-02 1.420875e-01 22 36488269 36488271 3 - 2.329 2.384 0.182
ENSG00000100350 E005 250.287436 1.538197e-03 1.097957e-02 3.111360e-02 22 36488272 36488276 5 - 2.345 2.420 0.252
ENSG00000100350 E006 772.503523 2.230882e-04 2.268706e-31 4.164723e-29 22 36488277 36488504 228 - 2.768 2.940 0.574
ENSG00000100350 E007 366.118635 1.452101e-04 5.831494e-39 1.885254e-36 22 36488505 36488598 94 - 2.378 2.642 0.881
ENSG00000100350 E008 4061.031772 7.138963e-05 2.071766e-10 3.461416e-09 22 36488599 36489986 1388 - 3.574 3.613 0.128
ENSG00000100350 E009 1411.123500 1.711655e-04 4.048333e-02 9.190799e-02 22 36489987 36490267 281 - 3.152 3.129 -0.075
ENSG00000100350 E010 17.504142 1.059492e-03 2.771678e-01 4.166787e-01 22 36492541 36493498 958 - 1.196 1.286 0.314
ENSG00000100350 E011 15.955114 1.157002e-03 9.660524e-02 1.852705e-01 22 36493499 36493632 134 - 1.121 1.265 0.513
ENSG00000100350 E012 1085.235307 9.261355e-04 1.575416e-03 5.879437e-03 22 36493633 36493798 166 - 3.057 3.003 -0.179
ENSG00000100350 E013 391.398314 1.549692e-04 1.057906e-04 5.486219e-04 22 36493799 36493803 5 - 2.624 2.550 -0.245
ENSG00000100350 E014 1183.498220 7.128818e-04 1.746339e-06 1.376201e-05 22 36495967 36496208 242 - 3.106 3.031 -0.248
ENSG00000100350 E015 975.889718 1.230619e-03 3.923401e-04 1.745354e-03 22 36497991 36498156 166 - 3.019 2.951 -0.226
ENSG00000100350 E016 9.750018 4.584073e-02 8.296333e-01 8.930347e-01 22 36498157 36498397 241 - 1.028 1.002 -0.096
ENSG00000100350 E017 3.773254 3.890341e-01 7.748988e-01 8.549511e-01 22 36500882 36501240 359 - 0.784 0.583 -0.847
ENSG00000100350 E018 995.093061 2.335587e-03 1.210294e-05 7.888481e-05 22 36501241 36501407 167 - 3.048 2.943 -0.352
ENSG00000100350 E019 822.239507 2.419587e-03 2.892793e-07 2.686690e-06 22 36504098 36504367 270 - 2.979 2.848 -0.436
ENSG00000100350 E020 3.432151 4.796181e-03 6.433350e-01 7.559164e-01 22 36504368 36504430 63 - 0.588 0.657 0.303
ENSG00000100350 E021 1140.458971 2.941192e-03 1.232024e-09 1.793616e-08 22 36504515 36504766 252 - 3.137 2.976 -0.536
ENSG00000100350 E022 1037.017005 4.019370e-03 3.863359e-11 7.305867e-10 22 36505896 36506163 268 - 3.117 2.912 -0.680
ENSG00000100350 E023 565.810958 8.087765e-03 2.035544e-05 1.260056e-04 22 36506164 36506423 260 - 2.845 2.659 -0.617
ENSG00000100350 E024 547.584578 5.812735e-03 7.673642e-06 5.233641e-05 22 36506424 36506695 272 - 2.823 2.653 -0.566
ENSG00000100350 E025 385.091479 7.010040e-03 2.686875e-06 2.032991e-05 22 36506696 36506911 216 - 2.686 2.484 -0.673
ENSG00000100350 E026 113.803831 2.040707e-02 2.411597e-05 1.468421e-04 22 36506912 36507008 97 - 2.209 1.894 -1.056
ENSG00000100350 E027 107.233117 3.082526e-02 2.799064e-03 9.671949e-03 22 36507009 36507221 213 - 2.157 1.904 -0.848