ENSG00000100348

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216185 ENSG00000100348 HEK293_OSMI2_2hA HEK293_TMG_2hB TXN2 protein_coding protein_coding 242.0361 472.5884 124.9138 9.973351 0.8958572 -1.919566 143.18548 295.58224 62.993213 7.054486 1.8536816 -2.2301111 0.56285000 0.62536667 0.50413333 -0.121233333 9.102112e-09 1.403557e-09 FALSE TRUE
ENST00000403313 ENSG00000100348 HEK293_OSMI2_2hA HEK293_TMG_2hB TXN2 protein_coding protein_coding 242.0361 472.5884 124.9138 9.973351 0.8958572 -1.919566 14.76626 22.22417 12.103670 1.868050 0.2498577 -0.8761422 0.07037917 0.04693333 0.09693333 0.050000000 1.403557e-09 1.403557e-09 FALSE TRUE
ENST00000487725 ENSG00000100348 HEK293_OSMI2_2hA HEK293_TMG_2hB TXN2 protein_coding processed_transcript 242.0361 472.5884 124.9138 9.973351 0.8958572 -1.919566 12.00107 19.46934 5.134298 2.631434 0.6024678 -1.9208991 0.05207917 0.04103333 0.04103333 0.000000000 1.000000e+00 1.403557e-09   FALSE
MSTRG.22048.4 ENSG00000100348 HEK293_OSMI2_2hA HEK293_TMG_2hB TXN2 protein_coding   242.0361 472.5884 124.9138 9.973351 0.8958572 -1.919566 14.53254 28.02110 8.194057 1.549145 0.5035074 -1.7726188 0.06020000 0.05936667 0.06553333 0.006166667 7.222754e-01 1.403557e-09   FALSE
MSTRG.22048.5 ENSG00000100348 HEK293_OSMI2_2hA HEK293_TMG_2hB TXN2 protein_coding   242.0361 472.5884 124.9138 9.973351 0.8958572 -1.919566 50.79730 93.64077 32.014824 1.907238 1.4035028 -1.5481002 0.22520000 0.19833333 0.25650000 0.058166667 2.724474e-02 1.403557e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100348 E001 64.44087 3.959252e-04 9.473438e-03 2.747194e-02 22 36467046 36467057 12 - 1.529 1.697 0.571
ENSG00000100348 E002 173.90986 2.350750e-03 3.033274e-03 1.036423e-02 22 36467058 36467070 13 - 1.982 2.121 0.465
ENSG00000100348 E003 274.54342 2.002597e-03 7.181122e-02 1.463612e-01 22 36467071 36467079 9 - 2.239 2.309 0.234
ENSG00000100348 E004 1608.99000 1.413243e-03 4.066615e-09 5.384832e-08 22 36467080 36467326 247 - 2.951 3.082 0.435
ENSG00000100348 E005 592.83900 6.080325e-04 3.417557e-10 5.505492e-09 22 36467327 36467327 1 - 2.496 2.652 0.517
ENSG00000100348 E006 2119.26376 1.679438e-04 2.978613e-10 4.850347e-09 22 36467328 36467548 221 - 3.116 3.193 0.257
ENSG00000100348 E007 1259.77069 5.586068e-04 3.229822e-07 2.968418e-06 22 36467549 36467590 42 - 2.876 2.971 0.313
ENSG00000100348 E008 2397.14048 9.756172e-05 3.373706e-13 8.897933e-12 22 36467591 36467795 205 - 3.167 3.247 0.265
ENSG00000100348 E009 2541.41368 5.847949e-05 8.753590e-02 1.713977e-01 22 36467796 36467883 88 - 3.273 3.259 -0.045
ENSG00000100348 E010 2049.15977 6.621431e-05 4.944904e-03 1.577764e-02 22 36467884 36467917 34 - 3.190 3.163 -0.090
ENSG00000100348 E011 13.70936 2.341341e-02 5.614543e-01 6.892729e-01 22 36468602 36468717 116 - 1.085 1.016 -0.253
ENSG00000100348 E012 2990.05240 7.561857e-05 3.985171e-05 2.297911e-04 22 36476733 36476856 124 - 3.359 3.326 -0.112
ENSG00000100348 E013 3524.64199 1.753283e-04 6.485076e-14 1.893033e-12 22 36480575 36480748 174 - 3.457 3.392 -0.215
ENSG00000100348 E014 1522.15211 2.545314e-04 1.137666e-07 1.143806e-06 22 36480749 36480787 39 - 3.096 3.027 -0.229
ENSG00000100348 E015 1421.47568 3.606700e-04 1.246282e-03 4.797506e-03 22 36480788 36480837 50 - 3.046 3.001 -0.150
ENSG00000100348 E016 215.81888 1.402769e-03 1.353562e-16 5.730242e-15 22 36481143 36481563 421 - 2.409 2.136 -0.911
ENSG00000100348 E017 947.59778 8.348438e-04 2.961778e-01 4.373820e-01 22 36481564 36481640 77 - 2.848 2.829 -0.062