ENSG00000100324

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216160 ENSG00000100324 HEK293_OSMI2_2hA HEK293_TMG_2hB TAB1 protein_coding protein_coding 40.97456 64.06675 23.59778 3.504776 0.5678317 -1.440539 33.870826 53.835327 17.018841 2.3861762 0.7259141 -1.6608409 0.8110167 0.84126667 0.7208 -0.12046667 1.762520e-04 1.753358e-08 FALSE TRUE
ENST00000331454 ENSG00000100324 HEK293_OSMI2_2hA HEK293_TMG_2hB TAB1 protein_coding protein_coding 40.97456 64.06675 23.59778 3.504776 0.5678317 -1.440539 1.659641 1.420382 2.104024 0.1970296 0.1891522 0.5635916 0.0480500 0.02216667 0.0894 0.06723333 1.753358e-08 1.753358e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100324 E001 15.0367046 0.0156552164 7.798409e-01 8.584808e-01 22 39399775 39399777 3 + 1.067 1.128 0.221
ENSG00000100324 E002 16.2545738 0.0139637048 8.399984e-01 9.000808e-01 22 39399778 39399779 2 + 1.107 1.158 0.183
ENSG00000100324 E003 17.3303935 0.0056972455 7.565552e-01 8.416204e-01 22 39399780 39399783 4 + 1.177 1.172 -0.019
ENSG00000100324 E004 102.0449766 0.0009908562 2.520672e-01 3.883904e-01 22 39399784 39399835 52 + 1.942 1.920 -0.074
ENSG00000100324 E005 0.4428904 0.4008677382 9.130239e-01 9.488936e-01 22 39414730 39414879 150 + 0.000 0.145 8.463
ENSG00000100324 E006 8.6534086 0.0053686392 3.422974e-02 8.019890e-02 22 39414880 39415005 126 + 1.067 0.837 -0.862
ENSG00000100324 E007 223.0925672 0.0012863644 2.245891e-04 1.068716e-03 22 39415006 39415142 137 + 2.330 2.242 -0.292
ENSG00000100324 E008 301.7453736 0.0006514129 2.037383e-04 9.799083e-04 22 39415500 39415653 154 + 2.446 2.379 -0.225
ENSG00000100324 E009 1.4287184 0.0101748769 9.505114e-01 9.730256e-01 22 39416744 39416790 47 + 0.327 0.329 0.015
ENSG00000100324 E010 248.5658140 0.0042095058 7.683634e-02 1.544323e-01 22 39416791 39416877 87 + 2.343 2.303 -0.135
ENSG00000100324 E011 321.6545289 0.0011501053 4.473195e-04 1.956669e-03 22 39417711 39417849 139 + 2.474 2.407 -0.222
ENSG00000100324 E012 336.0095856 0.0001912246 1.307458e-05 8.450262e-05 22 39418732 39418845 114 + 2.494 2.424 -0.234
ENSG00000100324 E013 337.1481239 0.0001962051 2.619682e-04 1.224593e-03 22 39419519 39419630 112 + 2.485 2.431 -0.182
ENSG00000100324 E014 4.3899798 0.0036234160 4.309281e-02 9.672523e-02 22 39421735 39421826 92 + 0.856 0.583 -1.124
ENSG00000100324 E015 395.0694704 0.0001440010 2.116279e-02 5.394111e-02 22 39421827 39421971 145 + 2.528 2.507 -0.069
ENSG00000100324 E016 455.7238957 0.0001594632 7.749676e-01 8.549940e-01 22 39426703 39426925 223 + 2.546 2.579 0.110
ENSG00000100324 E017 159.6560516 0.0033178718 8.261669e-01 8.905983e-01 22 39428021 39428026 6 + 2.101 2.122 0.071
ENSG00000100324 E018 392.1751236 0.0005521827 2.523306e-01 3.886641e-01 22 39428027 39428183 157 + 2.506 2.508 0.006
ENSG00000100324 E019 2271.8097145 0.0010170432 3.080828e-19 1.801956e-17 22 39430015 39431882 1868 + 3.157 3.300 0.475
ENSG00000100324 E020 8.8078516 0.0019351818 7.385989e-01 8.284458e-01 22 39432431 39432453 23 + 0.856 0.922 0.255
ENSG00000100324 E021 21.1852776 0.0184973892 9.824535e-02 1.878587e-01 22 39432454 39432663 210 + 1.370 1.229 -0.494
ENSG00000100324 E022 25.3627766 0.0759400422 9.057789e-02 1.760386e-01 22 39436490 39436567 78 + 1.489 1.290 -0.689
ENSG00000100324 E023 37.3296515 0.0113437052 2.188952e-03 7.807846e-03 22 39436568 39436795 228 + 1.656 1.445 -0.723
ENSG00000100324 E024 28.6288698 0.0016710049 6.911244e-02 1.419096e-01 22 39436796 39437060 265 + 1.472 1.363 -0.374