ENSG00000100321

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000318801 ENSG00000100321 HEK293_OSMI2_2hA HEK293_TMG_2hB SYNGR1 protein_coding protein_coding 66.09394 121.5463 45.50808 11.52184 1.085064 -1.417113 38.718944 76.870516 22.7716887 5.0580633 1.2956471 -1.7547433 0.56782083 0.63640000 0.5006667 -0.13573333 0.024490498 0.001259966 FALSE TRUE
ENST00000328933 ENSG00000100321 HEK293_OSMI2_2hA HEK293_TMG_2hB SYNGR1 protein_coding protein_coding 66.09394 121.5463 45.50808 11.52184 1.085064 -1.417113 5.520170 6.857487 5.3088216 0.3206942 0.3761253 -0.3686757 0.09562500 0.05753333 0.1169667 0.05943333 0.001259966 0.001259966 FALSE TRUE
ENST00000406293 ENSG00000100321 HEK293_OSMI2_2hA HEK293_TMG_2hB SYNGR1 protein_coding protein_coding 66.09394 121.5463 45.50808 11.52184 1.085064 -1.417113 12.745949 24.141040 12.2412413 5.8729112 1.9222925 -0.9791574 0.19300000 0.19253333 0.2679333 0.07540000 0.482443130 0.001259966 FALSE TRUE
MSTRG.22147.10 ENSG00000100321 HEK293_OSMI2_2hA HEK293_TMG_2hB SYNGR1 protein_coding   66.09394 121.5463 45.50808 11.52184 1.085064 -1.417113 3.352518 5.375831 0.8682857 0.5806745 0.5358608 -2.6164067 0.05464583 0.04416667 0.0188000 -0.02536667 0.511085745 0.001259966 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100321 E001 0.2966881 0.0275388073 3.583111e-02   22 39349921 39349924 4 + 0.332 0.000 -12.299
ENSG00000100321 E002 0.2966881 0.0275388073 3.583111e-02   22 39349925 39349937 13 + 0.332 0.000 -13.718
ENSG00000100321 E003 0.2966881 0.0275388073 3.583111e-02   22 39349938 39349945 8 + 0.332 0.000 -13.718
ENSG00000100321 E004 0.2966881 0.0275388073 3.583111e-02   22 39349946 39349947 2 + 0.332 0.000 -13.718
ENSG00000100321 E005 0.6653823 0.0172671820 3.926605e-02 8.965381e-02 22 39349948 39349948 1 + 0.435 0.074 -3.217
ENSG00000100321 E006 1.0685319 0.0121649107 1.988640e-01 3.247887e-01 22 39349949 39349949 1 + 0.435 0.192 -1.631
ENSG00000100321 E007 43.4745590 0.0082190975 3.600242e-01 5.043331e-01 22 39349950 39349986 37 + 1.620 1.535 -0.292
ENSG00000100321 E008 61.6464762 0.0059460782 6.435277e-01 7.560481e-01 22 39349987 39349990 4 + 1.740 1.693 -0.157
ENSG00000100321 E009 133.2535739 0.0101257435 3.255289e-01 4.689592e-01 22 39349991 39350005 15 + 2.090 2.020 -0.235
ENSG00000100321 E010 509.8129394 0.0032167177 6.100120e-01 7.293925e-01 22 39350006 39350109 104 + 2.615 2.618 0.009
ENSG00000100321 E011 0.2214452 0.0377324594 1.000000e+00   22 39364170 39364296 127 + 0.000 0.074 9.867
ENSG00000100321 E012 23.4854557 0.0008483145 7.070824e-01 8.050836e-01 22 39373528 39373644 117 + 1.335 1.286 -0.174
ENSG00000100321 E013 1135.2030224 0.0005156153 4.233119e-02 9.532745e-02 22 39374316 39374553 238 + 2.953 2.966 0.042
ENSG00000100321 E014 13.6541262 0.0014176158 2.324615e-01 3.653700e-01 22 39374554 39374571 18 + 1.186 1.048 -0.496
ENSG00000100321 E015 6.5397160 0.0082042184 1.266061e-02 3.511169e-02 22 39374572 39374660 89 + 1.031 0.690 -1.324
ENSG00000100321 E016 30.4355251 0.0097469810 9.686451e-07 8.058445e-06 22 39374661 39375935 1275 + 1.687 1.281 -1.396
ENSG00000100321 E017 11.0720195 0.0134124823 2.478637e-01 3.834135e-01 22 39375936 39376051 116 + 1.115 0.961 -0.563
ENSG00000100321 E018 504.0160071 0.0001301990 8.440992e-05 4.489264e-04 22 39376052 39376101 50 + 2.566 2.621 0.184
ENSG00000100321 E019 780.7875993 0.0001319121 2.328288e-08 2.679589e-07 22 39376102 39376197 96 + 2.747 2.813 0.219
ENSG00000100321 E020 3.8976724 0.0677312961 7.677749e-03 2.297183e-02 22 39376198 39376210 13 + 0.955 0.478 -1.998
ENSG00000100321 E021 8.7838956 0.0019552305 1.371331e-01 2.441013e-01 22 39377015 39377075 61 + 1.054 0.862 -0.716
ENSG00000100321 E022 1201.8192853 0.0017943874 1.313999e-09 1.905198e-08 22 39377598 39378389 792 + 2.903 3.011 0.360
ENSG00000100321 E023 28.0255102 0.0011222619 2.893831e-04 1.336652e-03 22 39381696 39381761 66 + 1.570 1.294 -0.951
ENSG00000100321 E024 1064.0571678 0.0020390526 3.143351e-20 2.058411e-18 22 39381762 39385575 3814 + 3.090 2.886 -0.680