ENSG00000100320

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000405409 ENSG00000100320 HEK293_OSMI2_2hA HEK293_TMG_2hB RBFOX2 protein_coding protein_coding 35.66384 28.75739 44.42391 1.879764 0.3106639 0.6272267 6.346630 7.225960 4.617636 1.0843355 0.05974445 -0.6449091 0.1880333 0.2502333 0.1039667 -0.14626667 5.795803e-06 5.795803e-06 FALSE TRUE
ENST00000438146 ENSG00000100320 HEK293_OSMI2_2hA HEK293_TMG_2hB RBFOX2 protein_coding protein_coding 35.66384 28.75739 44.42391 1.879764 0.3106639 0.6272267 11.676717 8.816989 14.610886 1.1583441 1.30487997 0.7280374 0.3287750 0.3050000 0.3287333 0.02373333 8.541730e-01 5.795803e-06 FALSE TRUE
ENST00000473487 ENSG00000100320 HEK293_OSMI2_2hA HEK293_TMG_2hB RBFOX2 protein_coding protein_coding 35.66384 28.75739 44.42391 1.879764 0.3106639 0.6272267 7.790737 4.962875 11.742675 0.5340911 0.61876482 1.2408372 0.2108125 0.1748333 0.2642000 0.08936667 1.018146e-01 5.795803e-06 FALSE FALSE
ENST00000695854 ENSG00000100320 HEK293_OSMI2_2hA HEK293_TMG_2hB RBFOX2 protein_coding protein_coding 35.66384 28.75739 44.42391 1.879764 0.3106639 0.6272267 7.730220 6.351264 11.282795 0.5740756 1.42960167 0.8280172 0.2129708 0.2216667 0.2542000 0.03253333 7.697204e-01 5.795803e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100320 E001 19.0295681 0.0009801519 1.022801e-07 1.036740e-06 22 35738736 35738757 22 - 1.003 1.499 1.752
ENSG00000100320 E002 4089.3707157 0.0005814627 4.587485e-03 1.478514e-02 22 35738758 35743601 4844 - 3.573 3.652 0.261
ENSG00000100320 E003 260.9831622 0.0024036167 3.104552e-13 8.223547e-12 22 35743602 35743932 331 - 2.495 2.319 -0.586
ENSG00000100320 E004 53.6763856 0.0049168567 2.245881e-02 5.663565e-02 22 35743933 35743957 25 - 1.772 1.698 -0.251
ENSG00000100320 E005 39.6658243 0.0026807049 2.497653e-01 3.856424e-01 22 35743958 35743962 5 - 1.613 1.604 -0.030
ENSG00000100320 E006 46.8474588 0.0006659791 1.156204e-01 2.137240e-01 22 35743963 35743982 20 - 1.690 1.671 -0.063
ENSG00000100320 E007 98.8419244 0.0036909373 8.533013e-01 9.091876e-01 22 35743983 35744077 95 - 1.967 2.033 0.224
ENSG00000100320 E008 66.6196869 0.0013005251 2.148146e-01 3.442249e-01 22 35744078 35744086 9 - 1.772 1.891 0.401
ENSG00000100320 E009 80.1616642 0.0016100940 6.220924e-01 7.389321e-01 22 35744087 35744124 38 - 1.893 1.934 0.138
ENSG00000100320 E010 78.8483526 0.0004828057 5.991056e-02 1.264568e-01 22 35744125 35744147 23 - 1.910 1.897 -0.043
ENSG00000100320 E011 85.3608079 0.0003148198 3.626549e-05 2.111691e-04 22 35744148 35744163 16 - 1.984 1.889 -0.318
ENSG00000100320 E012 120.9375726 0.0002379875 4.661908e-02 1.031517e-01 22 35744164 35744232 69 - 2.091 2.091 0.000
ENSG00000100320 E013 80.9618070 0.0003120145 2.311507e-01 3.638410e-01 22 35744233 35744249 17 - 1.910 1.927 0.057
ENSG00000100320 E014 103.6002489 0.0003132463 6.494582e-01 7.607283e-01 22 35745923 35745995 73 - 2.003 2.051 0.162
ENSG00000100320 E015 114.7458882 0.0016559867 9.212498e-01 9.543047e-01 22 35746473 35746561 89 - 2.036 2.103 0.227
ENSG00000100320 E016 11.7616487 0.0027674413 2.710879e-06 2.049599e-05 22 35746562 35750183 3622 - 1.256 0.820 -1.604
ENSG00000100320 E017 0.6674587 0.0338587677 3.860997e-01 5.302891e-01 22 35750184 35750260 77 - 0.268 0.138 -1.186
ENSG00000100320 E018 0.3686942 0.0347119583 9.244405e-01 9.564036e-01 22 35750426 35750457 32 - 0.108 0.138 0.399
ENSG00000100320 E019 0.3268771 0.0300509825 9.285498e-01   22 35752613 35752655 43 - 0.108 0.138 0.396
ENSG00000100320 E020 98.8488118 0.0036604658 6.102747e-01 7.296191e-01 22 35756105 35756144 40 - 1.984 2.020 0.122
ENSG00000100320 E021 171.0806271 0.0002128204 2.509980e-01 3.871366e-01 22 35759888 35760009 122 - 2.223 2.255 0.109
ENSG00000100320 E022 92.7551540 0.0003138064 1.515057e-02 4.082830e-02 22 35760010 35760020 11 - 1.988 1.962 -0.086
ENSG00000100320 E023 42.9577184 0.0012529101 2.356407e-02 5.891467e-02 22 35760021 35760032 12 - 1.674 1.612 -0.213
ENSG00000100320 E024 151.1847878 0.0011052338 6.857796e-03 2.085543e-02 22 35761202 35761294 93 - 2.197 2.171 -0.087
ENSG00000100320 E025 136.6100489 0.0092438328 9.368675e-02 1.808837e-01 22 35761415 35761468 54 - 2.157 2.115 -0.139
ENSG00000100320 E026 0.4720498 0.2250463164 7.144837e-01 8.106787e-01 22 35763437 35763556 120 - 0.195 0.138 -0.597
ENSG00000100320 E027 143.1587960 0.0164060031 1.293022e-01 2.332056e-01 22 35765423 35765483 61 - 2.183 2.128 -0.183
ENSG00000100320 E028 0.2966881 0.0271554937 2.426033e-01   22 35767368 35767441 74 - 0.195 0.000 -10.643
ENSG00000100320 E029 110.8172235 0.0119601432 8.552091e-02 1.682470e-01 22 35768257 35768267 11 - 2.073 2.017 -0.188
ENSG00000100320 E030 177.5979671 0.0037195840 2.684360e-04 1.251215e-03 22 35768268 35768341 74 - 2.292 2.203 -0.296
ENSG00000100320 E031 107.1514387 0.0003014528 1.316061e-07 1.306934e-06 22 35768342 35768349 8 - 2.090 1.971 -0.399
ENSG00000100320 E032 0.0000000       22 35772931 35772971 41 -      
ENSG00000100320 E033 2.3775439 0.0061890120 1.586198e-05 1.006601e-04 22 35777656 35778024 369 - 0.744 0.000 -14.152
ENSG00000100320 E034 100.3279819 0.0003113261 1.077881e-08 1.321179e-07 22 35778025 35778027 3 - 2.071 1.929 -0.475
ENSG00000100320 E035 147.1665176 0.0010227614 2.599193e-07 2.435379e-06 22 35778028 35778075 48 - 2.220 2.113 -0.358
ENSG00000100320 E036 91.9015244 0.0003328426 5.088189e-06 3.615476e-05 22 35778076 35778078 3 - 2.018 1.912 -0.358
ENSG00000100320 E037 234.0120471 0.0015728188 1.297296e-07 1.289516e-06 22 35781600 35781743 144 - 2.416 2.318 -0.328
ENSG00000100320 E038 35.0289733 0.0007594390 9.567271e-03 2.770207e-02 22 35781744 35781746 3 - 1.601 1.510 -0.311
ENSG00000100320 E039 239.2897864 0.0006725700 2.653164e-17 1.224443e-15 22 35809780 35810004 225 - 2.451 2.293 -0.526
ENSG00000100320 E040 0.0000000       22 35824320 35824373 54 -      
ENSG00000100320 E041 1.6273656 0.0343487697 1.990895e-01 3.250855e-01 22 35836320 35836439 120 - 0.515 0.325 -1.028
ENSG00000100320 E042 473.6756285 0.0174339883 1.538270e-52 1.079336e-49 22 35838287 35838684 398 - 1.810 2.965 3.859
ENSG00000100320 E043 16.4363995 0.0023460009 8.787981e-01 9.261553e-01 22 35840192 35840587 396 - 1.220 1.268 0.168
ENSG00000100320 E044 68.3401282 0.0003865366 2.540467e-12 5.835865e-11 22 35938847 35938897 51 - 1.942 1.698 -0.824
ENSG00000100320 E045 0.0000000       22 35961463 35961534 72 -      
ENSG00000100320 E046 0.0000000       22 35961563 35961666 104 -      
ENSG00000100320 E047 97.4020152 0.0345322010 7.377265e-04 3.034832e-03 22 36028240 36028824 585 - 2.102 1.828 -0.920