ENSG00000100299

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216124 ENSG00000100299 HEK293_OSMI2_2hA HEK293_TMG_2hB ARSA protein_coding protein_coding 14.40435 17.06846 12.5769 0.93411 0.2122113 -0.4402542 2.727643 2.722498 1.6117584 0.1585900 0.07227545 -0.7526615 0.18901250 0.15960000 0.12800000 -0.03160000 0.31065527 0.00872634 FALSE TRUE
ENST00000356098 ENSG00000100299 HEK293_OSMI2_2hA HEK293_TMG_2hB ARSA protein_coding protein_coding 14.40435 17.06846 12.5769 0.93411 0.2122113 -0.4402542 3.531554 4.190037 3.7737164 1.1543998 0.61068860 -0.1505983 0.24233750 0.24773333 0.30060000 0.05286667 0.78089113 0.00872634 FALSE TRUE
ENST00000395621 ENSG00000100299 HEK293_OSMI2_2hA HEK293_TMG_2hB ARSA protein_coding protein_coding 14.40435 17.06846 12.5769 0.93411 0.2122113 -0.4402542 0.964639 2.299880 1.1126860 0.3124356 1.11268595 -1.0408632 0.07328750 0.13686667 0.08576667 -0.05110000 0.22901263 0.00872634 FALSE TRUE
ENST00000453344 ENSG00000100299 HEK293_OSMI2_2hA HEK293_TMG_2hB ARSA protein_coding protein_coding 14.40435 17.06846 12.5769 0.93411 0.2122113 -0.4402542 1.457034 2.840473 1.3098111 0.9788822 0.20100632 -1.1108698 0.09032083 0.16443333 0.10473333 -0.05970000 0.68536473 0.00872634 FALSE TRUE
ENST00000551731 ENSG00000100299 HEK293_OSMI2_2hA HEK293_TMG_2hB ARSA protein_coding retained_intron 14.40435 17.06846 12.5769 0.93411 0.2122113 -0.4402542 3.912683 2.811847 3.6156992 0.2437187 0.29395178 0.3616194 0.27451250 0.16456667 0.28840000 0.12383333 0.00872634 0.00872634 FALSE TRUE
MSTRG.22380.7 ENSG00000100299 HEK293_OSMI2_2hA HEK293_TMG_2hB ARSA protein_coding   14.40435 17.06846 12.5769 0.93411 0.2122113 -0.4402542 1.328209 1.598367 0.9091492 0.2858064 0.18384075 -0.8072256 0.09625417 0.09243333 0.07283333 -0.01960000 0.71956098 0.00872634 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100299 E001 0.2965864 0.2281782800 4.270889e-01   22 50620663 50620720 58 - 0.000 0.177 9.725
ENSG00000100299 E002 8.1229424 0.0258855946 8.158914e-01 0.8834650853 22 50622754 50622872 119 - 0.904 0.943 0.148
ENSG00000100299 E003 14.5380185 0.0145751501 1.824909e-01 0.3043449227 22 50622873 50623027 155 - 1.053 1.205 0.544
ENSG00000100299 E004 43.2652353 0.0049399240 1.041835e-02 0.0297934301 22 50623028 50623773 746 - 1.498 1.671 0.587
ENSG00000100299 E005 8.9766627 0.0025375079 9.395946e-01 0.9660774023 22 50623774 50623895 122 - 0.985 0.977 -0.030
ENSG00000100299 E006 23.7614475 0.0011334765 1.313253e-01 0.2359917372 22 50623896 50624169 274 - 1.291 1.405 0.396
ENSG00000100299 E007 80.4353630 0.0003604255 9.798761e-01 0.9914630125 22 50624170 50625017 848 - 1.881 1.883 0.006
ENSG00000100299 E008 20.9599604 0.0079284540 7.113019e-03 0.0215184049 22 50625018 50625027 10 - 1.444 1.218 -0.788
ENSG00000100299 E009 19.9831161 0.0044004318 4.953420e-03 0.0158010826 22 50625028 50625028 1 - 1.424 1.196 -0.797
ENSG00000100299 E010 20.9010776 0.0011420916 2.434376e-03 0.0085647324 22 50625029 50625032 4 - 1.444 1.216 -0.796
ENSG00000100299 E011 150.9838617 0.0024626954 4.769077e-01 0.6155133233 22 50625033 50625362 330 - 2.139 2.167 0.093
ENSG00000100299 E012 112.2801485 0.0004065717 5.146485e-03 0.0163275984 22 50625363 50625464 102 - 1.960 2.060 0.335
ENSG00000100299 E013 126.3582307 0.0007523898 1.950408e-02 0.0504222074 22 50625579 50625681 103 - 2.024 2.105 0.273
ENSG00000100299 E014 140.7311203 0.0002595580 7.512345e-05 0.0004048213 22 50625936 50626063 128 - 2.036 2.161 0.420
ENSG00000100299 E015 144.1327392 0.0002299946 5.395379e-02 0.1161864068 22 50626154 50626278 125 - 2.096 2.155 0.198
ENSG00000100299 E016 150.3391853 0.0004253218 4.343996e-02 0.0973637019 22 50626591 50626760 170 - 2.193 2.134 -0.197
ENSG00000100299 E017 13.7012434 0.0160037145 1.357781e-02 0.0372566835 22 50626761 50626833 73 - 1.300 1.042 -0.922
ENSG00000100299 E018 115.0705785 0.0003274310 7.002194e-02 0.1434040395 22 50626834 50626989 156 - 2.078 2.018 -0.200
ENSG00000100299 E019 63.7571596 0.0032577936 3.444534e-01 0.4885449822 22 50626990 50627052 63 - 1.815 1.767 -0.160
ENSG00000100299 E020 13.1261992 0.0122929544 2.702201e-02 0.0660436720 22 50627053 50627165 113 - 1.253 1.025 -0.818
ENSG00000100299 E021 139.8462854 0.0027124541 1.818682e-01 0.3035689513 22 50627166 50627406 241 - 2.083 2.137 0.183
ENSG00000100299 E022 141.2257709 0.0003094706 5.422377e-01 0.6730259468 22 50627556 50627792 237 - 2.139 2.122 -0.059
ENSG00000100299 E023 74.1113207 0.0003970908 1.249439e-01 0.2270418588 22 50627793 50627897 105 - 1.893 1.830 -0.212
ENSG00000100299 E024 54.0960798 0.0004297962 8.485937e-02 0.1671816298 22 50627898 50627945 48 - 1.769 1.686 -0.280
ENSG00000100299 E025 75.9794157 0.0003738330 3.764139e-02 0.0866575223 22 50627946 50627990 45 - 1.913 1.828 -0.285
ENSG00000100299 E026 91.6124533 0.0003034796 4.146242e-02 0.0937398528 22 50627991 50628173 183 - 1.989 1.914 -0.253