ENSG00000100281

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216106 ENSG00000100281 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGXB4 protein_coding protein_coding 19.36429 8.742486 31.83775 0.941262 0.3185744 1.863427 7.139143 4.1112157 10.985776 0.3774013 1.8455252 1.415808 0.38979583 0.47503333 0.3451000 -0.1299333 3.791467e-01 5.295009e-58 FALSE TRUE
ENST00000418170 ENSG00000100281 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGXB4 protein_coding nonsense_mediated_decay 19.36429 8.742486 31.83775 0.941262 0.3185744 1.863427 2.426927 0.1300682 5.863816 0.1300682 0.8195739 5.390097 0.08793333 0.01633333 0.1838667 0.1675333 2.793553e-02 5.295009e-58 FALSE TRUE
ENST00000455359 ENSG00000100281 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGXB4 protein_coding protein_coding 19.36429 8.742486 31.83775 0.941262 0.3185744 1.863427 2.568195 0.0000000 6.406465 0.0000000 0.1896092 9.325635 0.08380417 0.00000000 0.2012667 0.2012667 6.032200e-34 5.295009e-58 FALSE TRUE
MSTRG.22022.1 ENSG00000100281 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGXB4 protein_coding   19.36429 8.742486 31.83775 0.941262 0.3185744 1.863427 3.518125 1.4630269 7.134034 0.7503739 0.8445301 2.277955 0.16189167 0.16100000 0.2242000 0.0632000 7.824149e-01 5.295009e-58 FALSE TRUE
MSTRG.22022.2 ENSG00000100281 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGXB4 protein_coding   19.36429 8.742486 31.83775 0.941262 0.3185744 1.863427 2.733565 2.5459770 0.000000 0.3171570 0.0000000 -7.997731 0.21576250 0.29066667 0.0000000 -0.2906667 5.295009e-58 5.295009e-58 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100281 E001 0.3697384 0.0274424043 3.656234e-02 8.464654e-02 22 35257430 35257451 22 + 0.000 0.342 11.795
ENSG00000100281 E002 1.1813376 0.0173012841 8.233371e-02 1.631918e-01 22 35257452 35257486 35 + 0.185 0.531 2.175
ENSG00000100281 E003 1.3285866 0.0102436278 1.439755e-01 2.534236e-01 22 35257487 35257488 2 + 0.232 0.531 1.760
ENSG00000100281 E004 1.3285866 0.0102436278 1.439755e-01 2.534236e-01 22 35257489 35257492 4 + 0.232 0.531 1.760
ENSG00000100281 E005 3.2087203 0.0048392096 6.547994e-01 7.648848e-01 22 35257493 35257495 3 + 0.541 0.662 0.538
ENSG00000100281 E006 75.3874213 0.0003939668 1.385295e-06 1.115600e-05 22 35257496 35257557 62 + 1.868 1.695 -0.587
ENSG00000100281 E007 4.3522928 0.0055306042 1.948502e-01 3.198186e-01 22 35258083 35258588 506 + 0.603 0.845 0.995
ENSG00000100281 E008 3.4126897 0.0233893969 7.810747e-01 8.593396e-01 22 35261854 35262322 469 + 0.603 0.601 -0.008
ENSG00000100281 E009 57.8434863 0.0034150388 2.167992e-05 1.333026e-04 22 35262323 35262421 99 + 1.762 1.553 -0.709
ENSG00000100281 E010 1.3263991 0.0560385507 3.071197e-01 4.493013e-01 22 35262763 35263077 315 + 0.383 0.203 -1.244
ENSG00000100281 E011 140.0702971 0.0002872694 1.182144e-10 2.061998e-09 22 35263078 35263226 149 + 2.134 1.967 -0.562
ENSG00000100281 E012 109.0176292 0.0005572658 1.102885e-06 9.074011e-06 22 35263796 35263874 79 + 2.020 1.883 -0.458
ENSG00000100281 E013 50.9867885 0.0043681073 4.148123e-04 1.831177e-03 22 35263982 35264096 115 + 1.705 1.530 -0.596
ENSG00000100281 E014 434.7840740 0.0001618432 7.212198e-08 7.527155e-07 22 35264648 35265603 956 + 2.596 2.561 -0.116
ENSG00000100281 E015 79.5280648 0.0003782897 4.709945e-01 6.101719e-01 22 35283962 35284043 82 + 1.847 1.880 0.110
ENSG00000100281 E016 89.8563831 0.0003245978 2.383996e-02 5.948043e-02 22 35285997 35286061 65 + 1.915 1.883 -0.106
ENSG00000100281 E017 3.0225617 0.0086797962 6.688803e-01 7.757984e-01 22 35286882 35287346 465 + 0.541 0.661 0.535
ENSG00000100281 E018 113.0440331 0.0002917127 4.494141e-01 5.906194e-01 22 35287347 35287452 106 + 1.997 2.034 0.124
ENSG00000100281 E019 161.4776478 0.0002220222 2.239315e-02 5.649264e-02 22 35288238 35288407 170 + 2.161 2.155 -0.021
ENSG00000100281 E020 152.3767351 0.0007690161 1.298075e-02 3.586565e-02 22 35292992 35293114 123 + 2.139 2.119 -0.068
ENSG00000100281 E021 964.6745997 0.0023971765 2.908207e-20 1.915019e-18 22 35293607 35295807 2201 + 2.863 3.080 0.723