ENSG00000100258

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000474879 ENSG00000100258 HEK293_OSMI2_2hA HEK293_TMG_2hB LMF2 protein_coding protein_coding 58.97301 65.69327 47.30059 5.587311 1.974433 -0.4738022 36.071671 46.385182 26.833858 3.5809265 1.0188900 -0.7893830 0.60610417 0.70700000 0.56800000 -0.13900000 3.326836e-06 7.79571e-09 FALSE TRUE
ENST00000504717 ENSG00000100258 HEK293_OSMI2_2hA HEK293_TMG_2hB LMF2 protein_coding retained_intron 58.97301 65.69327 47.30059 5.587311 1.974433 -0.4738022 4.942251 6.056368 3.671203 0.9123271 0.2129135 -0.7206558 0.08189583 0.09140000 0.07756667 -0.01383333 6.122185e-01 7.79571e-09 TRUE TRUE
ENST00000507607 ENSG00000100258 HEK293_OSMI2_2hA HEK293_TMG_2hB LMF2 protein_coding retained_intron 58.97301 65.69327 47.30059 5.587311 1.974433 -0.4738022 8.323048 5.014500 7.485907 0.7096682 0.4749405 0.5771231 0.14517917 0.07576667 0.15796667 0.08220000 7.795710e-09 7.79571e-09 FALSE FALSE
ENST00000514938 ENSG00000100258 HEK293_OSMI2_2hA HEK293_TMG_2hB LMF2 protein_coding retained_intron 58.97301 65.69327 47.30059 5.587311 1.974433 -0.4738022 5.899705 3.619210 6.344501 0.8385652 0.6592503 0.8081232 0.10460833 0.05403333 0.13383333 0.07980000 2.242625e-04 7.79571e-09 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100258 E001 5.027046 0.0033450311 8.043928e-01 8.755670e-01 22 50502949 50502950 2 - 0.773 0.740 -0.131
ENSG00000100258 E002 229.541235 0.0062719660 2.403191e-01 3.745074e-01 22 50502951 50503057 107 - 2.284 2.345 0.205
ENSG00000100258 E003 415.626823 0.0071675532 3.573969e-02 8.307988e-02 22 50503058 50503230 173 - 2.510 2.612 0.339
ENSG00000100258 E004 177.627756 0.0012816747 9.613427e-08 9.791059e-07 22 50503231 50503260 30 - 2.074 2.262 0.628
ENSG00000100258 E005 371.453565 0.0022892535 1.040349e-06 8.599457e-06 22 50503261 50503389 129 - 2.421 2.575 0.513
ENSG00000100258 E006 699.614529 0.0002687767 2.012602e-09 2.814853e-08 22 50503390 50503699 310 - 2.735 2.834 0.328
ENSG00000100258 E007 359.732980 0.0002017141 3.132629e-07 2.887075e-06 22 50503808 50503904 97 - 2.436 2.547 0.370
ENSG00000100258 E008 395.891272 0.0001694286 2.900249e-06 2.177480e-05 22 50504340 50504434 95 - 2.488 2.584 0.319
ENSG00000100258 E009 231.487543 0.0002004629 3.797141e-05 2.200743e-04 22 50504435 50504451 17 - 2.245 2.355 0.367
ENSG00000100258 E010 29.421512 0.0008450375 2.338471e-01 3.669479e-01 22 50504452 50504558 107 - 1.491 1.413 -0.269
ENSG00000100258 E011 494.089796 0.0002137497 1.003398e-02 2.884489e-02 22 50504559 50504727 169 - 2.619 2.668 0.163
ENSG00000100258 E012 314.407535 0.0001787592 6.407584e-01 7.539596e-01 22 50504802 50504833 32 - 2.462 2.455 -0.026
ENSG00000100258 E013 517.505299 0.0001386872 4.486510e-02 9.995364e-02 22 50504834 50504984 151 - 2.696 2.665 -0.104
ENSG00000100258 E014 368.596416 0.0001344177 8.930267e-02 1.740765e-01 22 50505057 50505153 97 - 2.549 2.518 -0.104
ENSG00000100258 E015 36.009072 0.0016675725 1.019141e-10 1.795362e-09 22 50505154 50505228 75 - 1.755 1.367 -1.328
ENSG00000100258 E016 355.234816 0.0001639047 7.967835e-04 3.247903e-03 22 50505229 50505334 106 - 2.555 2.490 -0.216
ENSG00000100258 E017 36.958153 0.0053255521 9.336349e-13 2.303351e-11 22 50505335 50505402 68 - 1.807 1.336 -1.611
ENSG00000100258 E018 379.042823 0.0002803360 3.714495e-05 2.157530e-04 22 50505403 50505537 135 - 2.592 2.513 -0.266
ENSG00000100258 E019 177.757770 0.0015439502 4.421678e-02 9.878899e-02 22 50505674 50505695 22 - 2.253 2.191 -0.209
ENSG00000100258 E020 336.225409 0.0001445127 4.192646e-01 5.625083e-01 22 50505696 50505815 120 - 2.497 2.482 -0.048
ENSG00000100258 E021 398.561206 0.0001800620 2.392914e-02 5.965502e-02 22 50506035 50506213 179 - 2.588 2.547 -0.136
ENSG00000100258 E022 10.100729 0.0271998968 2.135155e-04 1.021952e-03 22 50506214 50506220 7 - 1.277 0.810 -1.717
ENSG00000100258 E023 303.813861 0.0004003902 6.627677e-02 1.371812e-01 22 50506285 50506395 111 - 2.469 2.430 -0.131
ENSG00000100258 E024 232.786504 0.0024727118 2.647650e-01 4.027536e-01 22 50506396 50506502 107 - 2.353 2.320 -0.113
ENSG00000100258 E025 121.965888 0.0044716361 2.688882e-01 4.073543e-01 22 50506638 50506666 29 - 2.081 2.034 -0.156
ENSG00000100258 E026 332.038100 0.0043879972 1.118561e-02 3.160967e-02 22 50506782 50507035 254 - 2.544 2.455 -0.296
ENSG00000100258 E027 48.101607 0.0014117143 6.101373e-07 5.307589e-06 22 50507036 50507120 85 - 1.822 1.559 -0.890
ENSG00000100258 E028 29.642388 0.0006517366 2.992545e-08 3.368960e-07 22 50507121 50507142 22 - 1.660 1.310 -1.205
ENSG00000100258 E029 53.192581 0.0004334018 1.411471e-16 5.963400e-15 22 50507143 50507250 108 - 1.928 1.530 -1.346
ENSG00000100258 E030 156.611302 0.0055873752 1.843194e-05 1.153005e-04 22 50507582 50507702 121 - 2.283 2.090 -0.645