ENSG00000100228

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263116 ENSG00000100228 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB36 protein_coding protein_coding 8.418352 12.35186 7.062548 0.983952 0.1748291 -0.8055935 4.5964205 8.3966263 2.5513379 0.4113679 0.2488391 -1.7146294 0.52397083 0.68460000 0.3599333 -0.32466667 1.082213e-05 1.082213e-05 FALSE TRUE
ENST00000420895 ENSG00000100228 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB36 protein_coding protein_coding 8.418352 12.35186 7.062548 0.983952 0.1748291 -0.8055935 0.6196867 0.9679698 0.8505732 0.1950836 0.1027096 -0.1844920 0.07432917 0.08186667 0.1212000 0.03933333 5.576056e-01 1.082213e-05 FALSE TRUE
MSTRG.21747.2 ENSG00000100228 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB36 protein_coding   8.418352 12.35186 7.062548 0.983952 0.1748291 -0.8055935 2.2087042 1.6140972 2.6093595 0.9660564 0.2552379 0.6895761 0.27695833 0.12160000 0.3682333 0.24663333 4.001071e-02 1.082213e-05 FALSE TRUE
MSTRG.21747.3 ENSG00000100228 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB36 protein_coding   8.418352 12.35186 7.062548 0.983952 0.1748291 -0.8055935 0.8460604 1.1557540 1.0370568 0.2268517 0.2678640 -0.1549237 0.10400000 0.09530000 0.1485000 0.05320000 5.917449e-01 1.082213e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100228 E001 0.9117310 0.1096780113 0.6827939865 0.7861700319 22 23145366 23145417 52 + 0.164 0.287 1.033
ENSG00000100228 E002 4.0302944 0.1337582792 0.9804658134 0.9917660290 22 23145418 23145476 59 + 0.623 0.692 0.294
ENSG00000100228 E003 37.3772901 0.0018292603 0.5114792597 0.6465052797 22 23145477 23145551 75 + 1.507 1.556 0.169
ENSG00000100228 E004 9.9234146 0.0017604241 0.5306307051 0.6632068449 22 23145959 23146030 72 + 0.987 1.001 0.054
ENSG00000100228 E005 52.7592477 0.0005555171 0.0201349079 0.0517669285 22 23146605 23146685 81 + 1.707 1.679 -0.093
ENSG00000100228 E006 54.2951982 0.0006747196 0.0433293842 0.0971676020 22 23150063 23150112 50 + 1.710 1.700 -0.037
ENSG00000100228 E007 51.7037684 0.0004854013 0.0161651963 0.0430899796 22 23150113 23150154 42 + 1.703 1.671 -0.109
ENSG00000100228 E008 69.0402099 0.0030057203 0.0255149229 0.0629422596 22 23152461 23152526 66 + 1.816 1.798 -0.064
ENSG00000100228 E009 82.7210716 0.0032541486 0.0037182543 0.0123496577 22 23153033 23153134 102 + 1.911 1.868 -0.145
ENSG00000100228 E010 68.2438475 0.0049763594 0.0019536004 0.0070826475 22 23155968 23156032 65 + 1.851 1.779 -0.246
ENSG00000100228 E011 8.5697224 0.0343428780 0.9076600308 0.9453810578 22 23156033 23156311 279 + 0.896 0.968 0.270
ENSG00000100228 E012 51.0489333 0.0016271347 0.0091320609 0.0266131578 22 23157992 23158043 52 + 1.707 1.658 -0.165
ENSG00000100228 E013 59.3310582 0.0118538704 0.0223173047 0.0563343118 22 23158898 23158979 82 + 1.778 1.721 -0.194
ENSG00000100228 E014 54.7661758 0.0052797385 0.0728009264 0.1480061957 22 23159163 23159253 91 + 1.714 1.701 -0.045
ENSG00000100228 E015 51.3548396 0.0086427802 0.0932586029 0.1802168873 22 23160879 23160998 120 + 1.690 1.673 -0.060
ENSG00000100228 E016 821.9696506 0.0143538561 0.0001515253 0.0007538541 22 23161500 23165663 4164 + 2.716 2.927 0.702
ENSG00000100228 E017 0.2924217 0.0290785164 0.0504484826   22 23168897 23168958 62 + 0.282 0.000 -17.207