ENSG00000100225

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000266087 ENSG00000100225 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO7 protein_coding protein_coding 108.3062 164.9921 85.43408 10.57455 1.357608 -0.949432 48.285195 61.611742 46.0990684 6.122238 1.3862068 -0.4183889 0.45557917 0.37526667 0.539433333 0.1641666667 9.883501e-03 3.97737e-08 FALSE TRUE
ENST00000397426 ENSG00000100225 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO7 protein_coding protein_coding 108.3062 164.9921 85.43408 10.57455 1.357608 -0.949432 28.683348 52.344530 16.7525066 7.058250 2.1764490 -1.6430765 0.26355833 0.31656667 0.196166667 -0.1204000000 8.032309e-02 3.97737e-08 FALSE TRUE
ENST00000420700 ENSG00000100225 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO7 protein_coding nonsense_mediated_decay 108.3062 164.9921 85.43408 10.57455 1.357608 -0.949432 11.745975 16.503257 8.5259598 1.521841 1.1309527 -0.9519994 0.10698750 0.10086667 0.100133333 -0.0007333333 1.000000e+00 3.97737e-08 FALSE TRUE
ENST00000452138 ENSG00000100225 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO7 protein_coding protein_coding 108.3062 164.9921 85.43408 10.57455 1.357608 -0.949432 6.074581 13.149855 4.5936699 3.647152 1.4031511 -1.5152874 0.05150833 0.07770000 0.053466667 -0.0242333333 6.690428e-01 3.97737e-08 FALSE TRUE
MSTRG.22000.2 ENSG00000100225 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO7 protein_coding   108.3062 164.9921 85.43408 10.57455 1.357608 -0.949432 8.537019 10.907144 6.9193700 1.340363 0.4035287 -0.6557994 0.08012500 0.06643333 0.080900000 0.0144666667 5.366625e-01 3.97737e-08 FALSE TRUE
MSTRG.22000.9 ENSG00000100225 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO7 protein_coding   108.3062 164.9921 85.43408 10.57455 1.357608 -0.949432 3.760498 9.741109 0.6311633 1.939704 0.1873783 -3.9268025 0.02882500 0.05860000 0.007433333 -0.0511666667 8.237844e-06 3.97737e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100225 E001 15.4285681 0.0012410630 8.251660e-03 2.442124e-02 22 32474676 32474724 49 + 1.314 1.099 -0.761
ENSG00000100225 E002 57.4632603 0.0110816712 1.386959e-01 2.462999e-01 22 32474725 32474736 12 + 1.784 1.693 -0.307
ENSG00000100225 E003 162.2367551 0.0192688685 2.317277e-01 3.645094e-01 22 32474737 32474797 61 + 2.221 2.146 -0.249
ENSG00000100225 E004 161.5843777 0.0225875491 1.967233e-01 3.221515e-01 22 32474798 32474810 13 + 2.227 2.140 -0.289
ENSG00000100225 E005 379.0495486 0.0144417193 1.899694e-02 4.932721e-02 22 32474811 32475014 204 + 2.623 2.495 -0.425
ENSG00000100225 E006 344.2419753 0.0054532235 3.389505e-05 1.989564e-04 22 32475015 32475124 110 + 2.599 2.444 -0.514
ENSG00000100225 E007 0.6568746 0.0199740247 7.765335e-01 8.560934e-01 22 32475125 32475236 112 + 0.166 0.225 0.542
ENSG00000100225 E008 0.5085815 0.2407491550 9.469277e-01 9.706779e-01 22 32475237 32475251 15 + 0.166 0.163 -0.032
ENSG00000100225 E009 0.3634088 0.3724658188 5.986542e-01   22 32475252 32475254 3 + 0.000 0.163 9.516
ENSG00000100225 E010 0.5117019 0.0230579611 2.804142e-01 4.202697e-01 22 32475255 32475256 2 + 0.000 0.225 10.090
ENSG00000100225 E011 57.7011665 0.0171716891 3.449133e-01 4.890086e-01 22 32475257 32475398 142 + 1.769 1.710 -0.201
ENSG00000100225 E012 1.8885502 0.0173856437 1.337133e-01 2.393158e-01 22 32475593 32475957 365 + 0.580 0.330 -1.301
ENSG00000100225 E013 1.9272745 0.0264832198 3.573389e-03 1.193729e-02 22 32478035 32478196 162 + 0.716 0.226 -2.624
ENSG00000100225 E014 730.5728686 0.0012411907 2.069155e-07 1.977883e-06 22 32478981 32479132 152 + 2.887 2.792 -0.316
ENSG00000100225 E015 1015.5594348 0.0002601258 9.743444e-12 2.034237e-10 22 32479133 32479275 143 + 3.017 2.944 -0.245
ENSG00000100225 E016 1373.4435253 0.0001648670 4.517032e-07 4.030417e-06 22 32483897 32484124 228 + 3.125 3.086 -0.128
ENSG00000100225 E017 1022.5861624 0.0001716418 8.112209e-01 8.802056e-01 22 32485068 32485209 142 + 2.960 2.974 0.049
ENSG00000100225 E018 4.9197333 0.0032724441 1.098960e-05 7.232749e-05 22 32487288 32487744 457 + 1.052 0.483 -2.331
ENSG00000100225 E019 740.9089968 0.0001197003 1.383524e-01 2.458120e-01 22 32487745 32487828 84 + 2.802 2.842 0.132
ENSG00000100225 E020 44.0211833 0.0029285883 4.007760e-38 1.224610e-35 22 32487829 32491085 3257 + 1.994 1.250 -2.542
ENSG00000100225 E021 999.1518377 0.0003449654 1.811138e-03 6.634336e-03 22 32491086 32491181 96 + 2.914 2.976 0.207
ENSG00000100225 E022 19.8848174 0.0127710128 1.131855e-13 3.190397e-12 22 32491182 32493104 1923 + 1.639 0.961 -2.384
ENSG00000100225 E023 1438.8528818 0.0010255329 1.627668e-04 8.038497e-04 22 32493105 32493281 177 + 3.058 3.137 0.263
ENSG00000100225 E024 651.1889557 0.0005497913 2.622519e-07 2.456506e-06 22 32495493 32495530 38 + 2.689 2.803 0.377
ENSG00000100225 E025 2768.4158891 0.0022781444 6.973005e-08 7.298828e-07 22 32498144 32498829 686 + 3.308 3.431 0.409