ENSG00000100221

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216039 ENSG00000100221 HEK293_OSMI2_2hA HEK293_TMG_2hB JOSD1 protein_coding protein_coding 42.69615 47.02996 40.26173 2.004083 1.437754 -0.2241195 34.227549 42.25052 29.325085 1.6025910 0.8760749 -0.5266834 0.7975583 0.89896667 0.73093333 -0.16803333 0.003785274 0.003785274 FALSE TRUE
ENST00000683374 ENSG00000100221 HEK293_OSMI2_2hA HEK293_TMG_2hB JOSD1 protein_coding protein_coding 42.69615 47.02996 40.26173 2.004083 1.437754 -0.2241195 3.543224 2.58196 4.108186 0.7046056 0.6518144 0.6679655 0.0828250 0.05443333 0.10200000 0.04756667 0.227273179 0.003785274 FALSE TRUE
MSTRG.22125.7 ENSG00000100221 HEK293_OSMI2_2hA HEK293_TMG_2hB JOSD1 protein_coding   42.69615 47.02996 40.26173 2.004083 1.437754 -0.2241195 2.012440 0.00000 2.101277 0.0000000 2.1012765 7.7219717 0.0507875 0.00000000 0.04873333 0.04873333 0.840491196 0.003785274 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100221 E001 2079.1924280 0.0053525316 5.595940e-08 5.965192e-07 22 38685543 38687089 1547 - 3.189 3.373 0.613
ENSG00000100221 E002 155.6378557 0.0002326540 2.766874e-02 6.735317e-02 22 38687090 38687139 50 - 2.115 2.228 0.376
ENSG00000100221 E003 317.8217882 0.0002269021 4.296975e-03 1.397562e-02 22 38687140 38687323 184 - 2.427 2.535 0.359
ENSG00000100221 E004 658.5065039 0.0002080055 7.026778e-01 8.014895e-01 22 38687324 38687717 394 - 2.785 2.826 0.139
ENSG00000100221 E005 465.5981623 0.0020939996 9.300229e-02 1.798133e-01 22 38687718 38687923 206 - 2.658 2.661 0.011
ENSG00000100221 E006 293.4843987 0.0002333836 3.898429e-04 1.735468e-03 22 38687924 38688001 78 - 2.476 2.448 -0.095
ENSG00000100221 E007 335.6562257 0.0003550798 2.259991e-03 8.030245e-03 22 38688935 38689073 139 - 2.526 2.510 -0.055
ENSG00000100221 E008 201.9820653 0.0001982962 1.761961e-04 8.619710e-04 22 38689074 38689129 56 - 2.328 2.281 -0.157
ENSG00000100221 E009 8.5292388 0.0054015699 6.630067e-04 2.763214e-03 22 38689130 38689295 166 - 1.150 0.814 -1.252
ENSG00000100221 E010 274.8421042 0.0002029332 1.722956e-06 1.359300e-05 22 38689296 38689424 129 - 2.464 2.407 -0.190
ENSG00000100221 E011 0.7782011 0.0157098419 4.151585e-01 5.585618e-01 22 38699801 38699802 2 - 0.321 0.199 -0.913
ENSG00000100221 E012 486.0266119 0.0006985686 1.180607e-06 9.657689e-06 22 38699803 38700123 321 - 2.706 2.658 -0.160
ENSG00000100221 E013 160.0430143 0.0064948462 5.551836e-02 1.188665e-01 22 38700124 38700160 37 - 2.223 2.181 -0.142
ENSG00000100221 E014 166.3746112 0.0061628442 2.450021e-01 3.800374e-01 22 38700161 38700275 115 - 2.218 2.213 -0.019
ENSG00000100221 E015 43.3821292 0.0005196368 3.890161e-13 1.017365e-11 22 38700276 38700317 42 - 1.809 1.470 -1.155
ENSG00000100221 E016 198.3155360 0.0141300775 8.080283e-07 6.844252e-06 22 38700318 38700618 301 - 2.427 2.166 -0.869
ENSG00000100221 E017 37.0019725 0.0434408590 1.914055e-02 4.964499e-02 22 38700619 38700667 49 - 1.687 1.465 -0.759
ENSG00000100221 E018 16.0847348 0.0954445553 6.097965e-02 1.282832e-01 22 38700800 38700861 62 - 1.345 1.113 -0.822
ENSG00000100221 E019 10.9932666 0.0354679544 6.628395e-03 2.025893e-02 22 38700862 38700903 42 - 1.222 0.908 -1.144
ENSG00000100221 E020 12.7460993 0.0372430933 4.764205e-04 2.069959e-03 22 38700904 38700943 40 - 1.324 0.908 -1.502
ENSG00000100221 E021 3.7897012 0.0360266677 3.101438e-02 7.394389e-02 22 38700966 38701256 291 - 0.835 0.521 -1.332
ENSG00000100221 E022 17.5048895 0.0161581560 2.178774e-01 3.478675e-01 22 38701257 38702055 799 - 1.300 1.218 -0.286