ENSG00000100219

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216037 ENSG00000100219 HEK293_OSMI2_2hA HEK293_TMG_2hB XBP1 protein_coding protein_coding 124.3498 160.0815 114.004 12.78517 0.9378721 -0.4896865 112.001174 149.954577 99.848095 12.0623242 0.6193679 -0.5866705 0.8989792 0.93663333 0.87593333 -0.0607 4.574542e-06 4.574542e-06 FALSE  
ENST00000344347 ENSG00000100219 HEK293_OSMI2_2hA HEK293_TMG_2hB XBP1 protein_coding protein_coding 124.3498 160.0815 114.004 12.78517 0.9378721 -0.4896865 7.398108 5.947093 7.974215 0.5998355 0.9250104 0.4225419 0.0598125 0.03723333 0.06983333 0.0326 6.406120e-03 4.574542e-06 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100219 E001 6.027730 0.0038219263 5.514206e-03 1.732306e-02 22 28794555 28794556 2 - 1.028 0.680 -1.351
ENSG00000100219 E002 7.060571 0.0045644722 1.076556e-01 2.020790e-01 22 28794557 28794558 2 - 1.010 0.816 -0.736
ENSG00000100219 E003 141.706350 0.0012152189 6.905739e-07 5.937243e-06 22 28794559 28794588 30 - 2.009 2.186 0.591
ENSG00000100219 E004 2743.306729 0.0004062942 1.283546e-30 2.257789e-28 22 28794589 28795174 586 - 3.342 3.447 0.349
ENSG00000100219 E005 3510.348776 0.0008460428 7.305153e-01 8.224485e-01 22 28795175 28795732 558 - 3.524 3.519 -0.016
ENSG00000100219 E006 591.825765 0.0001208432 2.775432e-01 4.171206e-01 22 28796047 28796121 75 - 2.766 2.746 -0.068
ENSG00000100219 E007 313.702250 0.0001473388 7.339183e-01 8.250364e-01 22 28796122 28796147 26 - 2.474 2.478 0.011
ENSG00000100219 E008 492.563537 0.0001747579 9.495998e-03 2.752725e-02 22 28796148 28796192 45 - 2.703 2.655 -0.161
ENSG00000100219 E009 63.177701 0.0003830523 5.194883e-20 3.301426e-18 22 28796193 28797045 853 - 2.013 1.606 -1.374
ENSG00000100219 E010 891.322761 0.0012916844 9.911883e-04 3.928074e-03 22 28797077 28797205 129 - 2.975 2.899 -0.251
ENSG00000100219 E011 2.171308 0.0108914196 4.977315e-02 1.087586e-01 22 28798906 28799041 136 - 0.679 0.354 -1.583
ENSG00000100219 E012 6.291656 0.0185930434 1.037981e-01 1.962975e-01 22 28799042 28799056 15 - 0.973 0.745 -0.880
ENSG00000100219 E013 774.637899 0.0021568431 1.701038e-05 1.072040e-04 22 28799057 28799153 97 - 2.939 2.822 -0.388
ENSG00000100219 E014 5.188896 0.0036906702 3.807240e-02 8.748500e-02 22 28799960 28800297 338 - 0.932 0.656 -1.096
ENSG00000100219 E015 620.692403 0.0111492663 3.823232e-04 1.706323e-03 22 28800298 28800597 300 - 2.887 2.693 -0.646