ENSG00000100154

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397906 ENSG00000100154 HEK293_OSMI2_2hA HEK293_TMG_2hB TTC28 protein_coding protein_coding 6.215914 5.043639 8.192959 0.4027829 0.3145392 0.6988219 2.7307219 1.2976863 5.2579415 0.4543296 0.6309588 2.010223 0.42205000 0.24786667 0.6491000 0.40123333 0.03328029 0.0006195304 FALSE TRUE
ENST00000480563 ENSG00000100154 HEK293_OSMI2_2hA HEK293_TMG_2hB TTC28 protein_coding retained_intron 6.215914 5.043639 8.192959 0.4027829 0.3145392 0.6988219 0.3049277 0.1075068 0.8810538 0.1075068 0.3074389 2.922769 0.04440833 0.01936667 0.1049000 0.08553333 0.15639592 0.0006195304 FALSE FALSE
ENST00000612946 ENSG00000100154 HEK293_OSMI2_2hA HEK293_TMG_2hB TTC28 protein_coding protein_coding 6.215914 5.043639 8.192959 0.4027829 0.3145392 0.6988219 2.5974724 3.4293121 1.0333160 0.2140994 0.6284826 -1.720944 0.44427500 0.68913333 0.1207333 -0.56840000 0.07648838 0.0006195304 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100154 E001 1026.4965519 0.0131752658 1.844713e-13 5.056691e-12 22 27978014 27983598 5585 - 2.796 3.206 1.363
ENSG00000100154 E002 55.2259548 0.0004127477 1.961953e-02 5.067226e-02 22 27983599 27983751 153 - 1.713 1.767 0.183
ENSG00000100154 E003 47.7350346 0.0008279431 3.354800e-02 7.886885e-02 22 27983752 27983851 100 - 1.651 1.705 0.184
ENSG00000100154 E004 46.4269756 0.0012433707 5.826840e-01 7.069793e-01 22 27985249 27985356 108 - 1.604 1.739 0.461
ENSG00000100154 E005 36.1932600 0.0005907485 8.237655e-01 8.889618e-01 22 27989878 27990007 130 - 1.490 1.641 0.516
ENSG00000100154 E006 2.3356104 0.0066516727 6.945233e-02 1.424531e-01 22 27990008 27990076 69 - 0.576 0.377 -1.001
ENSG00000100154 E007 13.4324299 0.0053792853 7.889809e-01 8.649037e-01 22 27990789 27990812 24 - 1.073 1.255 0.652
ENSG00000100154 E008 22.5147359 0.0008527130 2.071163e-01 3.350702e-01 22 27992587 27992611 25 - 1.328 1.397 0.240
ENSG00000100154 E009 27.8797173 0.0038887470 9.272718e-02 1.793821e-01 22 27992612 27992663 52 - 1.428 1.471 0.148
ENSG00000100154 E010 46.0378723 0.0198187682 1.816362e-01 3.032861e-01 22 27993287 27993518 232 - 1.635 1.695 0.204
ENSG00000100154 E011 32.3656262 0.0103093805 5.324846e-01 6.648539e-01 22 27996135 27996259 125 - 1.464 1.582 0.406
ENSG00000100154 E012 1.8863747 0.0075331609 5.229852e-01 6.567371e-01 22 27996260 27996424 165 - 0.454 0.453 -0.002
ENSG00000100154 E013 103.1181428 0.0003667716 5.284797e-04 2.265851e-03 22 27998540 27999260 721 - 1.983 2.027 0.149
ENSG00000100154 E014 0.4815130 0.0206349476 4.056305e-01 5.492966e-01 22 27999261 28001373 2113 - 0.090 0.283 1.997
ENSG00000100154 E015 35.4499108 0.0005904520 1.039504e-01 1.965242e-01 22 28001374 28001553 180 - 1.515 1.583 0.231
ENSG00000100154 E016 37.6725581 0.0192090073 3.244507e-01 4.678079e-01 22 28014248 28014392 145 - 1.534 1.617 0.286
ENSG00000100154 E017 0.1482932 0.0424122114 3.668170e-01   22 28028815 28029116 302 - 0.000 0.164 20.554
ENSG00000100154 E018 44.5597717 0.0256711610 4.354739e-01 5.777135e-01 22 28030226 28030366 141 - 1.599 1.703 0.352
ENSG00000100154 E019 49.9900631 0.0061364600 2.637080e-01 4.016166e-01 22 28094080 28094245 166 - 1.649 1.749 0.341
ENSG00000100154 E020 59.7545126 0.0035343774 1.290427e-04 6.547071e-04 22 28096190 28096408 219 - 1.781 1.741 -0.135
ENSG00000100154 E021 48.0128672 0.0085376465 1.570814e-02 4.208066e-02 22 28098915 28099044 130 - 1.672 1.679 0.021
ENSG00000100154 E022 42.9980345 0.0198370260 5.144562e-02 1.117212e-01 22 28101171 28101280 110 - 1.623 1.630 0.024
ENSG00000100154 E023 85.2646910 0.0021885426 7.213033e-10 1.099435e-08 22 28105279 28105802 524 - 1.958 1.849 -0.367
ENSG00000100154 E024 144.5134988 0.0051894471 2.883633e-13 7.679163e-12 22 28107062 28108403 1342 - 2.200 2.040 -0.536
ENSG00000100154 E025 73.5172961 0.0003913230 1.301226e-20 8.947854e-19 22 28163092 28163599 508 - 1.933 1.685 -0.838
ENSG00000100154 E026 20.3236841 0.0009540340 1.896667e-07 1.825524e-06 22 28296198 28296213 16 - 1.397 1.128 -0.947
ENSG00000100154 E027 30.4347983 0.0006715497 4.533210e-10 7.156095e-09 22 28296214 28296328 115 - 1.562 1.298 -0.910
ENSG00000100154 E028 43.9064526 0.0005551895 6.663718e-13 1.683229e-11 22 28297580 28297852 273 - 1.711 1.464 -0.845
ENSG00000100154 E029 31.1130443 0.0071805948 2.976844e-07 2.757318e-06 22 28306496 28306643 148 - 1.565 1.329 -0.812
ENSG00000100154 E030 0.0000000       22 28306644 28306645 2 -      
ENSG00000100154 E031 0.8942170 0.0134447183 1.995463e-02 5.139000e-02 22 28442886 28443073 188 - 0.377 0.000 -21.421
ENSG00000100154 E032 0.8836053 0.0161307151 4.355812e-01 5.777992e-01 22 28629428 28629551 124 - 0.164 0.377 1.586
ENSG00000100154 E033 36.7273519 0.0219711817 3.681926e-05 2.140516e-04 22 28629552 28629830 279 - 1.630 1.411 -0.748
ENSG00000100154 E034 11.7573673 0.0284837476 4.602778e-03 1.482762e-02 22 28679622 28679840 219 - 1.163 0.946 -0.790