ENSG00000100099

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000336873 ENSG00000100099 HEK293_OSMI2_2hA HEK293_TMG_2hB HPS4 protein_coding protein_coding 22.97779 20.34002 24.12107 0.7740832 0.3563014 0.2458618 12.0477613 13.6056280 9.35273230 0.63027475 0.48567338 -0.5402620 0.52800000 0.66850000 0.388133333 -0.28036667 3.075889e-12 1.53632e-28 FALSE TRUE
ENST00000422379 ENSG00000100099 HEK293_OSMI2_2hA HEK293_TMG_2hB HPS4 protein_coding protein_coding 22.97779 20.34002 24.12107 0.7740832 0.3563014 0.2458618 1.8066287 0.0000000 3.50867733 0.00000000 0.28758499 8.4588894 0.07744167 0.00000000 0.145833333 0.14583333 1.536320e-28 1.53632e-28 FALSE TRUE
ENST00000429411 ENSG00000100099 HEK293_OSMI2_2hA HEK293_TMG_2hB HPS4 protein_coding nonsense_mediated_decay 22.97779 20.34002 24.12107 0.7740832 0.3563014 0.2458618 0.5072913 1.7931947 0.04742721 0.35604333 0.04742721 -4.9726770 0.02435833 0.08760000 0.001933333 -0.08566667 1.143181e-06 1.53632e-28 FALSE TRUE
ENST00000439453 ENSG00000100099 HEK293_OSMI2_2hA HEK293_TMG_2hB HPS4 protein_coding nonsense_mediated_decay 22.97779 20.34002 24.12107 0.7740832 0.3563014 0.2458618 1.4085195 0.1992164 2.40471159 0.10271181 0.40314010 3.5287833 0.05945833 0.01006667 0.099400000 0.08933333 4.756469e-03 1.53632e-28 FALSE TRUE
ENST00000464362 ENSG00000100099 HEK293_OSMI2_2hA HEK293_TMG_2hB HPS4 protein_coding nonsense_mediated_decay 22.97779 20.34002 24.12107 0.7740832 0.3563014 0.2458618 1.7896595 0.9593623 1.98237005 0.09848449 0.24648317 1.0393777 0.07640000 0.04766667 0.082466667 0.03480000 1.245048e-01 1.53632e-28 FALSE TRUE
MSTRG.21860.4 ENSG00000100099 HEK293_OSMI2_2hA HEK293_TMG_2hB HPS4 protein_coding   22.97779 20.34002 24.12107 0.7740832 0.3563014 0.2458618 1.3542918 0.7515260 1.34305041 0.13961881 0.35728810 0.8292504 0.05856667 0.03760000 0.055300000 0.01770000 6.355383e-01 1.53632e-28 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100099 E001 4.8565275 0.0034751756 2.740271e-02 6.681022e-02 22 26443423 26443552 130 - 0.567 0.882 1.302
ENSG00000100099 E002 6.8484067 0.0026793382 1.870489e-01 3.100291e-01 22 26444227 26444694 468 - 0.781 0.949 0.648
ENSG00000100099 E003 4.4609577 0.0114893441 5.678186e-01 6.945675e-01 22 26450882 26451286 405 - 0.781 0.707 -0.299
ENSG00000100099 E004 1.5111342 0.2033008806 4.995467e-01 6.358645e-01 22 26451287 26451479 193 - 0.302 0.492 1.067
ENSG00000100099 E005 4.6381908 0.0035207712 9.501912e-02 1.829069e-01 22 26451480 26451558 79 - 0.604 0.844 0.987
ENSG00000100099 E006 5.9312012 0.0028676795 3.715786e-02 8.577576e-02 22 26451559 26451620 62 - 0.671 0.948 1.093
ENSG00000100099 E007 4.5922078 0.0722430708 1.762481e-01 2.963571e-01 22 26451621 26451631 11 - 0.604 0.840 0.974
ENSG00000100099 E008 5.5499102 0.0453256004 2.254699e-01 3.569664e-01 22 26451632 26451655 24 - 0.701 0.896 0.773
ENSG00000100099 E009 13.7601413 0.0013496533 1.164658e-01 2.149094e-01 22 26451656 26451821 166 - 1.082 1.232 0.537
ENSG00000100099 E010 9.6653781 0.0024744105 1.079915e-01 2.025571e-01 22 26451822 26452082 261 - 0.924 1.102 0.659
ENSG00000100099 E011 461.0618282 0.0034513980 1.136875e-07 1.143133e-06 22 26452083 26452748 666 - 2.559 2.729 0.567
ENSG00000100099 E012 221.5510605 0.0005612044 4.966241e-07 4.394392e-06 22 26452749 26452915 167 - 2.262 2.400 0.460
ENSG00000100099 E013 201.2264854 0.0002745563 6.696105e-08 7.034252e-07 22 26452916 26453111 196 - 2.216 2.363 0.491
ENSG00000100099 E014 128.3304674 0.0002475945 3.529749e-04 1.591803e-03 22 26453112 26453218 107 - 2.040 2.162 0.405
ENSG00000100099 E015 169.2615626 0.0002855982 1.417076e-03 5.363850e-03 22 26453219 26453404 186 - 2.172 2.269 0.323
ENSG00000100099 E016 4.8628582 0.0033167317 1.481172e-01 2.591199e-01 22 26453646 26453972 327 - 0.639 0.845 0.836
ENSG00000100099 E017 120.5023626 0.0002442390 6.783834e-05 3.697181e-04 22 26457859 26457967 109 - 2.000 2.138 0.463
ENSG00000100099 E018 144.2567265 0.0004718445 7.379735e-04 3.035782e-03 22 26458445 26458577 133 - 2.094 2.206 0.373
ENSG00000100099 E019 247.6810631 0.0001979920 1.424704e-01 2.513933e-01 22 26463917 26464238 322 - 2.372 2.413 0.138
ENSG00000100099 E020 423.5906622 0.0004041867 4.669448e-03 1.501008e-02 22 26464239 26464826 588 - 2.652 2.609 -0.145
ENSG00000100099 E021 122.1403728 0.0002793469 8.180100e-02 1.623857e-01 22 26465455 26465520 66 - 2.115 2.067 -0.159
ENSG00000100099 E022 85.5097193 0.0003463773 4.596892e-01 5.998040e-01 22 26465521 26465551 31 - 1.946 1.926 -0.068
ENSG00000100099 E023 45.5678702 0.0005077736 3.593655e-15 1.262624e-13 22 26465552 26466050 499 - 1.848 1.439 -1.391
ENSG00000100099 E024 51.8114506 0.0004556402 3.952261e-05 2.281017e-04 22 26466051 26466104 54 - 1.820 1.630 -0.646
ENSG00000100099 E025 14.6530043 0.0012388886 1.674714e-07 1.629614e-06 22 26466105 26466225 121 - 1.395 0.933 -1.655
ENSG00000100099 E026 79.2007428 0.0003635026 2.101039e-01 3.386673e-01 22 26466226 26466262 37 - 1.929 1.888 -0.138
ENSG00000100099 E027 36.5427129 0.0005680178 6.199958e-13 1.574060e-11 22 26466263 26468550 2288 - 1.759 1.345 -1.417
ENSG00000100099 E028 87.9853028 0.0003432612 6.712901e-02 1.385818e-01 22 26468551 26468623 73 - 1.984 1.924 -0.202
ENSG00000100099 E029 90.2407303 0.0003119540 9.697469e-02 1.858626e-01 22 26470719 26470813 95 - 1.988 1.935 -0.179
ENSG00000100099 E030 13.5965903 0.0014688385 2.636757e-01 4.015835e-01 22 26470814 26470922 109 - 1.216 1.124 -0.329
ENSG00000100099 E031 12.1040042 0.0547407853 4.565885e-01 5.969812e-01 22 26471213 26471288 76 - 1.160 1.063 -0.350
ENSG00000100099 E032 12.6979834 0.0046241344 3.903039e-01 5.342913e-01 22 26471289 26471388 100 - 1.169 1.092 -0.276
ENSG00000100099 E033 118.1957441 0.0002867265 1.319507e-01 2.368325e-01 22 26472302 26472418 117 - 2.096 2.055 -0.138
ENSG00000100099 E034 129.4418609 0.0011802939 2.734430e-01 4.124767e-01 22 26472832 26472939 108 - 2.129 2.098 -0.104
ENSG00000100099 E035 1.8668940 0.5041787139 7.175707e-01 8.129069e-01 22 26472940 26472950 11 - 0.528 0.367 -0.838
ENSG00000100099 E036 9.4844460 0.0570190607 7.829392e-02 1.567359e-01 22 26475370 26476990 1621 - 1.148 0.869 -1.031
ENSG00000100099 E037 1.3974853 0.0168962269 9.195771e-01 9.532020e-01 22 26476991 26476992 2 - 0.370 0.355 -0.086
ENSG00000100099 E038 154.5730230 0.0039593104 3.219219e-01 4.651892e-01 22 26476993 26477136 144 - 2.209 2.172 -0.122
ENSG00000100099 E039 112.3311156 0.0005810165 2.628116e-01 4.006282e-01 22 26479265 26479355 91 - 2.070 2.039 -0.103
ENSG00000100099 E040 0.4375944 0.0260296033 7.420673e-02 1.502733e-01 22 26479356 26479681 326 - 0.303 0.000 -10.167
ENSG00000100099 E041 158.3103958 0.0002133705 5.192336e-04 2.231865e-03 22 26481722 26481834 113 - 2.249 2.161 -0.296
ENSG00000100099 E042 101.5913810 0.0002827182 2.868211e-05 1.714330e-04 22 26481835 26481857 23 - 2.081 1.943 -0.463
ENSG00000100099 E043 243.7074275 0.0019015957 2.696045e-06 2.039197e-05 22 26481858 26482240 383 - 2.456 2.321 -0.450
ENSG00000100099 E044 4.9036483 0.0033728205 4.490929e-01 5.902956e-01 22 26482746 26482873 128 - 0.827 0.733 -0.371
ENSG00000100099 E045 24.7334174 0.0088026424 4.216437e-02 9.502592e-02 22 26483674 26483744 71 - 1.489 1.327 -0.561
ENSG00000100099 E046 20.1672843 0.0029891969 5.483885e-03 1.724496e-02 22 26483745 26483764 20 - 1.429 1.216 -0.741
ENSG00000100099 E047 27.3020135 0.0158090687 8.066928e-02 1.605934e-01 22 26483765 26483873 109 - 1.530 1.375 -0.533