ENSG00000100030

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215832 ENSG00000100030 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK1 protein_coding protein_coding 34.79241 20.40856 49.74194 2.229125 0.6445318 1.284872 27.708937 18.692760 38.329874 2.1508124 0.7340397 1.035594 0.8143875 0.91516667 0.77050000 -0.14466667 2.938553e-07 1.747605e-13 FALSE TRUE
ENST00000398822 ENSG00000100030 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK1 protein_coding protein_coding 34.79241 20.40856 49.74194 2.229125 0.6445318 1.284872 4.837058 1.715795 8.910813 0.2621148 0.5582915 2.369914 0.1257292 0.08483333 0.17940000 0.09456667 1.648982e-03 1.747605e-13 FALSE TRUE
ENST00000491588 ENSG00000100030 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK1 protein_coding processed_transcript 34.79241 20.40856 49.74194 2.229125 0.6445318 1.284872 2.051722 0.000000 2.501252 0.0000000 0.4349987 7.972263 0.0560500 0.00000000 0.05006667 0.05006667 1.747605e-13 1.747605e-13   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100030 E001 3133.103733 0.0060828892 4.603268e-04 2.007725e-03 22 21759657 21763983 4327 - 3.425 3.560 0.448
ENSG00000100030 E002 466.398721 0.0020972523 9.755685e-01 9.887468e-01 22 21763984 21764240 257 - 2.628 2.693 0.216
ENSG00000100030 E003 15.196299 0.0031627284 7.022491e-07 6.027501e-06 22 21769040 21769194 155 - 0.951 1.459 1.810
ENSG00000100030 E004 178.206523 0.0001927770 2.844033e-01 4.246414e-01 22 21769195 21769203 9 - 2.201 2.302 0.338
ENSG00000100030 E005 343.897117 0.0002237496 1.824486e-01 3.042969e-01 22 21769204 21769320 117 - 2.505 2.550 0.147
ENSG00000100030 E006 1.819667 0.0083957887 1.597072e-01 2.747328e-01 22 21769321 21769428 108 - 0.506 0.293 -1.196
ENSG00000100030 E007 325.856921 0.0035658749 1.005776e-01 1.913335e-01 22 21772873 21772982 110 - 2.494 2.499 0.017
ENSG00000100030 E008 276.148930 0.0030445725 2.906202e-01 4.314449e-01 22 21788257 21788388 132 - 2.415 2.443 0.092
ENSG00000100030 E009 251.455179 0.0083362599 1.862877e-01 3.090878e-01 22 21788694 21788808 115 - 2.386 2.382 -0.013
ENSG00000100030 E010 283.160622 0.0028485470 6.810981e-02 1.402324e-01 22 21799012 21799128 117 - 2.435 2.441 0.020
ENSG00000100030 E011 391.819430 0.0001510453 2.263617e-09 3.141882e-08 22 21805850 21806039 190 - 2.593 2.551 -0.139
ENSG00000100030 E012 298.902166 0.0002888109 1.749372e-22 1.475571e-20 22 21807664 21807846 183 - 2.509 2.355 -0.514
ENSG00000100030 E013 181.698079 0.0063408492 1.623560e-16 6.807888e-15 22 21867322 21867680 359 - 2.339 1.989 -1.172