ENSG00000100029

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335214 ENSG00000100029 HEK293_OSMI2_2hA HEK293_TMG_2hB PES1 protein_coding protein_coding 254.7362 458.4116 165.5686 7.577214 0.7508561 -1.469158 18.089725 23.9783837 19.379383 0.9518752 3.372887 -0.3070691 0.08078750 0.0523666667 0.1169000 0.06453333 9.785957e-03 2.121891e-40 FALSE TRUE
ENST00000354694 ENSG00000100029 HEK293_OSMI2_2hA HEK293_TMG_2hB PES1 protein_coding protein_coding 254.7362 458.4116 165.5686 7.577214 0.7508561 -1.469158 202.303073 368.0472443 123.099287 0.9614885 1.742992 -1.5799906 0.78829583 0.8033333333 0.7435333 -0.05980000 8.593724e-02 2.121891e-40 FALSE TRUE
ENST00000441668 ENSG00000100029 HEK293_OSMI2_2hA HEK293_TMG_2hB PES1 protein_coding protein_coding 254.7362 458.4116 165.5686 7.577214 0.7508561 -1.469158 19.485532 56.8209116 0.000000 9.8072357 0.000000 -12.4724601 0.05127917 0.1233000000 0.0000000 -0.12330000 2.121891e-40 2.121891e-40 FALSE FALSE
MSTRG.21935.6 ENSG00000100029 HEK293_OSMI2_2hA HEK293_TMG_2hB PES1 protein_coding   254.7362 458.4116 165.5686 7.577214 0.7508561 -1.469158 3.732988 0.2086204 8.501198 0.2086204 1.201242 5.2828622 0.02379583 0.0004666667 0.0514000 0.05093333 3.640681e-04 2.121891e-40 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100029 E001 4.9733946 3.489421e-03 1.561707e-02 4.188230e-02 22 30576625 30576627 3 - 0.326 0.785 2.185
ENSG00000100029 E002 5.8150941 3.179888e-03 4.987172e-03 1.589350e-02 22 30576628 30576629 2 - 0.326 0.848 2.430
ENSG00000100029 E003 11.3678562 2.207053e-02 1.192019e-04 6.102322e-04 22 30576630 30576631 2 - 0.428 1.111 2.826
ENSG00000100029 E004 37.0845232 1.371198e-03 2.504147e-05 1.517993e-04 22 30576632 30576635 4 - 1.192 1.560 1.278
ENSG00000100029 E005 93.5823802 2.941448e-03 5.042048e-03 1.604490e-02 22 30576636 30576648 13 - 1.752 1.931 0.603
ENSG00000100029 E006 142.7566024 4.155721e-03 2.093498e-06 1.621584e-05 22 30576649 30576663 15 - 1.852 2.125 0.918
ENSG00000100029 E007 159.0933643 3.933758e-03 1.336241e-06 1.080024e-05 22 30576664 30576664 1 - 1.903 2.171 0.897
ENSG00000100029 E008 1313.9860623 3.139861e-03 9.260347e-13 2.286685e-11 22 30576665 30576834 170 - 2.834 3.079 0.816
ENSG00000100029 E009 4105.3736384 1.254900e-03 8.599616e-25 9.232492e-23 22 30576835 30577129 295 - 3.357 3.569 0.703
ENSG00000100029 E010 3983.4755584 1.395424e-03 1.455809e-20 9.953036e-19 22 30578837 30578995 159 - 3.353 3.554 0.670
ENSG00000100029 E011 1577.5422799 1.328741e-03 1.108355e-17 5.410319e-16 22 30578996 30578998 3 - 2.944 3.154 0.697
ENSG00000100029 E012 20.0574490 9.748602e-04 7.854863e-05 4.211167e-04 22 30578999 30579136 138 - 0.855 1.315 1.673
ENSG00000100029 E013 4303.6499185 7.088751e-04 1.399930e-13 3.895600e-12 22 30579137 30579303 167 - 3.447 3.576 0.430
ENSG00000100029 E014 31.7892451 6.558682e-03 4.804497e-06 3.435356e-05 22 30579304 30579750 447 - 1.661 1.351 -1.065
ENSG00000100029 E015 3681.9940775 1.340524e-04 1.363469e-03 5.187893e-03 22 30579751 30579935 185 - 3.439 3.495 0.184
ENSG00000100029 E016 29.8890316 6.795657e-04 2.095746e-04 1.004840e-03 22 30579936 30580052 117 - 1.573 1.346 -0.783
ENSG00000100029 E017 2527.7117500 4.843611e-05 1.551228e-01 2.685609e-01 22 30580053 30580178 126 - 3.288 3.329 0.135
ENSG00000100029 E018 2207.5021048 2.760337e-04 4.779124e-01 6.163870e-01 22 30580571 30580686 116 - 3.246 3.265 0.063
ENSG00000100029 E019 1099.9665315 1.169138e-03 4.987268e-01 6.351495e-01 22 30580687 30580701 15 - 2.948 2.961 0.043
ENSG00000100029 E020 1657.6905833 6.331300e-04 2.160650e-01 3.456644e-01 22 30581012 30581101 90 - 3.131 3.138 0.024
ENSG00000100029 E021 1536.2932073 3.189110e-04 3.929238e-19 2.272923e-17 22 30581334 30581408 75 - 3.172 3.082 -0.301
ENSG00000100029 E022 1780.2863551 1.452823e-04 3.770897e-48 2.076701e-45 22 30581528 30581644 117 - 3.265 3.135 -0.430
ENSG00000100029 E023 9.5801520 4.650815e-03 5.764079e-10 8.937531e-09 22 30584227 30584364 138 - 1.348 0.712 -2.357
ENSG00000100029 E024 1103.1706652 7.039173e-05 1.316629e-46 6.633816e-44 22 30584365 30584415 51 - 3.072 2.924 -0.493
ENSG00000100029 E025 1037.4289625 3.078825e-04 1.017026e-33 2.269406e-31 22 30584416 30584454 39 - 3.046 2.896 -0.498
ENSG00000100029 E026 2258.3587913 8.196381e-04 1.469965e-14 4.730431e-13 22 30584546 30584717 172 - 3.343 3.248 -0.313
ENSG00000100029 E027 18.5449949 1.309464e-02 1.340088e-06 1.082902e-05 22 30585224 30585370 147 - 1.503 1.066 -1.534
ENSG00000100029 E028 23.5886864 8.749287e-04 5.167346e-13 1.326654e-11 22 30586998 30587285 288 - 1.639 1.147 -1.708
ENSG00000100029 E029 2141.3191542 9.845047e-04 5.896003e-12 1.273057e-10 22 30587286 30587395 110 - 3.317 3.226 -0.301
ENSG00000100029 E030 2738.4484481 8.895154e-04 2.522306e-17 1.166610e-15 22 30588021 30588174 154 - 3.435 3.329 -0.352
ENSG00000100029 E031 1721.8269299 1.418378e-03 3.056526e-10 4.967399e-09 22 30589191 30589270 80 - 3.230 3.129 -0.336
ENSG00000100029 E032 2.2318085 1.860479e-01 2.506958e-01 3.867617e-01 22 30591794 30591809 16 - 0.193 0.496 1.930
ENSG00000100029 E033 1159.8346043 2.926916e-03 1.654254e-05 1.045516e-04 22 30591810 30591917 108 - 3.055 2.958 -0.322
ENSG00000100029 E034 0.3268771 3.087106e-02 4.034643e-01   22 30592132 30592398 267 - 0.193 0.075 -1.567
ENSG00000100029 E035 0.0000000       22 30603778 30603779 2 -      
ENSG00000100029 E036 0.7426990 3.456411e-02 1.000000e+00 1.000000e+00 22 30603780 30604102 323 - 0.193 0.196 0.021
ENSG00000100029 E037 0.1515154 4.358654e-02 1.460135e-01   22 30605414 30605460 47 - 0.193 0.000 -11.713
ENSG00000100029 E038 0.1515154 4.358654e-02 1.460135e-01   22 30605461 30605517 57 - 0.193 0.000 -11.713
ENSG00000100029 E039 0.1472490 4.352311e-02 1.461811e-01   22 30606809 30607083 275 - 0.193 0.000 -11.713