ENSG00000099999

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215798 ENSG00000099999 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF215 protein_coding protein_coding 20.592 28.03342 14.97499 1.68222 0.5601426 -0.9041443 2.676994 2.931197 2.7048131 0.22513009 0.1803043 -0.1155505 0.13395000 0.10453333 0.18193333 0.077400000 0.0082241109 0.0003068041 FALSE TRUE
ENST00000382363 ENSG00000099999 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF215 protein_coding protein_coding 20.592 28.03342 14.97499 1.68222 0.5601426 -0.9041443 12.878559 19.273472 8.5460472 1.06186717 0.3382403 -1.1723485 0.61699167 0.68790000 0.57100000 -0.116900000 0.0003068041 0.0003068041 FALSE TRUE
ENST00000463319 ENSG00000099999 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF215 protein_coding retained_intron 20.592 28.03342 14.97499 1.68222 0.5601426 -0.9041443 1.504229 1.702835 1.0113087 0.32829642 0.2552744 -0.7459669 0.07505833 0.06043333 0.06650000 0.006066667 0.9482448821 0.0003068041 FALSE TRUE
ENST00000473077 ENSG00000099999 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF215 protein_coding retained_intron 20.592 28.03342 14.97499 1.68222 0.5601426 -0.9041443 1.500340 1.398335 1.1125907 0.33460191 0.1465414 -0.3271589 0.07561667 0.04890000 0.07493333 0.026033333 0.3803037256 0.0003068041 FALSE FALSE
MSTRG.21929.3 ENSG00000099999 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF215 protein_coding   20.592 28.03342 14.97499 1.68222 0.5601426 -0.9041443 1.234450 1.880481 0.8688214 0.05801003 0.1557774 -1.1051112 0.05692917 0.06733333 0.05736667 -0.009966667 0.7282529546 0.0003068041   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099999 E001 18.824591 0.0009976395 0.0044424413 0.014378582 22 30368811 30374323 5513 - 1.400 1.170 -0.806
ENSG00000099999 E002 4.790188 0.1375716086 0.2346887302 0.367997383 22 30374324 30375155 832 - 0.899 0.639 -1.044
ENSG00000099999 E003 32.674166 0.0109983555 0.8706751013 0.920867938 22 30375156 30375752 597 - 1.485 1.469 -0.053
ENSG00000099999 E004 21.168844 0.0133967048 0.7175435550 0.812900504 22 30375753 30376020 268 - 1.316 1.278 -0.130
ENSG00000099999 E005 21.955327 0.0056860698 0.9227280844 0.955271881 22 30377511 30377819 309 - 1.305 1.313 0.028
ENSG00000099999 E006 10.481943 0.0118619050 0.7927540514 0.867500282 22 30377820 30377845 26 - 0.994 1.027 0.123
ENSG00000099999 E007 32.404290 0.0073125551 0.1042938299 0.197023954 22 30377846 30378142 297 - 1.373 1.506 0.458
ENSG00000099999 E008 93.605542 0.0024192037 0.0048537239 0.015524361 22 30378821 30378982 162 - 1.819 1.953 0.452
ENSG00000099999 E009 450.853454 0.0017430879 0.0225590506 0.056842291 22 30378983 30379481 499 - 2.559 2.615 0.187
ENSG00000099999 E010 164.397286 0.0011612166 0.0494535750 0.108186370 22 30379482 30379547 66 - 2.114 2.179 0.220
ENSG00000099999 E011 132.785769 0.0002869116 0.0880530810 0.172163025 22 30379548 30379589 42 - 2.028 2.086 0.192
ENSG00000099999 E012 110.710228 0.0002760119 0.2123435707 0.341298688 22 30379590 30379622 33 - 1.957 2.002 0.153
ENSG00000099999 E013 28.708802 0.0008533173 0.0389973536 0.089192834 22 30379623 30379710 88 - 1.518 1.377 -0.486
ENSG00000099999 E014 159.479744 0.0004728266 0.0345712519 0.080844062 22 30379711 30379813 103 - 2.098 2.166 0.225
ENSG00000099999 E015 152.714766 0.0002598885 0.0142719072 0.038843921 22 30380062 30380205 144 - 2.070 2.148 0.260
ENSG00000099999 E016 139.663837 0.0020890950 0.7880409824 0.864243414 22 30380282 30380401 120 - 2.097 2.086 -0.035
ENSG00000099999 E017 82.642439 0.0014352196 0.1207003349 0.220964752 22 30384339 30384369 31 - 1.912 1.842 -0.236
ENSG00000099999 E018 162.152464 0.0019471716 0.0033348486 0.011247789 22 30384370 30384495 126 - 2.227 2.125 -0.343
ENSG00000099999 E019 16.213102 0.0081838596 0.6078689748 0.727757894 22 30384496 30384834 339 - 1.211 1.160 -0.183
ENSG00000099999 E020 74.509734 0.0052625232 0.2495866462 0.385430369 22 30385904 30385906 3 - 1.868 1.804 -0.217
ENSG00000099999 E021 115.891765 0.0032428365 0.0984078732 0.188088404 22 30385907 30385989 83 - 2.063 1.991 -0.241
ENSG00000099999 E022 100.258840 0.0009342867 0.2395036348 0.373540057 22 30386070 30386141 72 - 1.980 1.932 -0.159
ENSG00000099999 E023 113.588026 0.0003439219 0.0108766098 0.030872767 22 30386616 30386759 144 - 2.065 1.974 -0.306
ENSG00000099999 E024 9.108786 0.0018618573 0.0645542514 0.134299810 22 30386760 30386886 127 - 1.090 0.885 -0.761
ENSG00000099999 E025 64.784588 0.0072951955 0.0003476835 0.001570969 22 30387029 30387411 383 - 1.904 1.690 -0.722
ENSG00000099999 E026 2.397145 0.1894349815 0.2530921627 0.389560947 22 30388155 30388279 125 - 0.649 0.406 -1.157
ENSG00000099999 E027 4.311627 0.0398355734 0.5836179940 0.707690162 22 30421661 30421771 111 - 0.597 0.692 0.409