ENSG00000099995

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215793 ENSG00000099995 HEK293_OSMI2_2hA HEK293_TMG_2hB SF3A1 protein_coding protein_coding 106.7727 114.6016 111.0449 10.17374 1.209413 -0.04547995 71.37385 94.24145 60.92895 2.393110 0.5405561 -0.6291501 0.6681458 0.8316333 0.5487333 -0.2829000 0.00520774 0.001332292 FALSE TRUE
MSTRG.21927.5 ENSG00000099995 HEK293_OSMI2_2hA HEK293_TMG_2hB SF3A1 protein_coding   106.7727 114.6016 111.0449 10.17374 1.209413 -0.04547995 32.47674 18.62672 45.81479 8.947553 1.2333503 1.2979799 0.3024375 0.1512333 0.4124667 0.2612333 0.06987869 0.001332292 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099995 E001 162.4188981 1.353898e-02 2.210583e-03 7.875663e-03 22 30331988 30332245 258 - 2.055 2.257 0.676
ENSG00000099995 E002 4497.9090136 2.831324e-03 8.108216e-08 8.382870e-07 22 30332246 30334695 2450 - 3.553 3.674 0.401
ENSG00000099995 E003 1079.9534170 8.923366e-05 2.405740e-23 2.206003e-21 22 30335467 30335538 72 - 2.935 3.051 0.385
ENSG00000099995 E004 1099.5462888 8.103105e-05 1.738918e-17 8.264744e-16 22 30335652 30335753 102 - 2.957 3.054 0.321
ENSG00000099995 E005 873.1517689 1.295411e-03 7.303146e-03 2.200772e-02 22 30337026 30337180 155 - 2.887 2.942 0.183
ENSG00000099995 E006 549.2123128 4.577767e-03 1.563290e-02 4.191817e-02 22 30337690 30337800 111 - 2.665 2.751 0.288
ENSG00000099995 E007 385.1377673 4.879424e-04 1.615891e-04 7.988251e-04 22 30337801 30337816 16 - 2.516 2.594 0.261
ENSG00000099995 E008 679.2256356 1.759545e-03 1.829166e-03 6.693258e-03 22 30337817 30337897 81 - 2.764 2.840 0.251
ENSG00000099995 E009 671.9091994 1.202351e-03 1.058842e-03 4.162572e-03 22 30338789 30338839 51 - 2.763 2.833 0.232
ENSG00000099995 E010 1046.8751121 1.585600e-04 2.593673e-06 1.969472e-05 22 30338840 30338960 121 - 2.964 3.020 0.187
ENSG00000099995 E011 895.3114836 8.832402e-05 7.975021e-06 5.417735e-05 22 30338961 30339034 74 - 2.898 2.952 0.179
ENSG00000099995 E012 4.7479762 3.438829e-03 6.169302e-01 7.348770e-01 22 30339035 30339129 95 - 0.696 0.764 0.274
ENSG00000099995 E013 849.4466623 9.457742e-05 8.336716e-01 8.958298e-01 22 30339130 30339179 50 - 2.914 2.908 -0.021
ENSG00000099995 E014 900.0078198 8.630011e-05 8.471880e-05 4.503665e-04 22 30339180 30339251 72 - 2.968 2.915 -0.176
ENSG00000099995 E015 559.7226606 1.006432e-04 4.503502e-04 1.967927e-03 22 30340196 30340208 13 - 2.766 2.707 -0.196
ENSG00000099995 E016 1280.5648179 1.072795e-04 5.607015e-04 2.387360e-03 22 30340209 30340381 173 - 3.113 3.072 -0.137
ENSG00000099995 E017 3.4665214 4.682345e-03 3.683328e-01 5.127531e-01 22 30340382 30340694 313 - 0.730 0.594 -0.576
ENSG00000099995 E018 1001.7060109 1.222438e-04 9.175123e-01 9.518842e-01 22 30340695 30340812 118 - 2.982 2.980 -0.008
ENSG00000099995 E019 1575.8509766 9.743913e-05 7.564065e-06 5.165601e-05 22 30341692 30341885 194 - 3.207 3.160 -0.159
ENSG00000099995 E020 1424.5921658 8.354452e-04 3.155012e-08 3.535267e-07 22 30342200 30342319 120 - 3.191 3.099 -0.308
ENSG00000099995 E021 813.7112493 1.103010e-03 4.907746e-08 5.300483e-07 22 30342320 30342350 31 - 2.960 2.847 -0.374
ENSG00000099995 E022 5.5982772 2.839747e-03 8.440974e-03 2.488744e-02 22 30342492 30342546 55 - 0.988 0.650 -1.333
ENSG00000099995 E023 6.4644808 2.651316e-03 6.773509e-04 2.816243e-03 22 30342547 30342574 28 - 1.069 0.650 -1.628
ENSG00000099995 E024 10.7145825 1.578622e-03 6.519570e-09 8.323986e-08 22 30342575 30342804 230 - 1.335 0.743 -2.188
ENSG00000099995 E025 972.9776283 2.791655e-03 4.145056e-04 1.829993e-03 22 30342805 30342879 75 - 3.030 2.931 -0.327
ENSG00000099995 E026 1.3411729 3.385947e-01 2.327998e-01 3.657591e-01 22 30344910 30344932 23 - 0.530 0.247 -1.644
ENSG00000099995 E027 1585.7251549 2.907603e-03 3.599428e-05 2.097705e-04 22 30344933 30345190 258 - 3.249 3.139 -0.367
ENSG00000099995 E028 2.6832212 2.033044e-02 5.852316e-01 7.089693e-01 22 30346221 30346311 91 - 0.475 0.566 0.434
ENSG00000099995 E029 1104.9928704 3.030891e-03 5.464969e-06 3.856379e-05 22 30346312 30346519 208 - 3.102 2.974 -0.426
ENSG00000099995 E030 0.2924217 2.713609e-02 1.151980e-01   22 30352545 30352950 406 - 0.254 0.000 -10.688
ENSG00000099995 E031 800.8187441 6.275076e-03 1.875692e-05 1.170899e-04 22 30352951 30353072 122 - 2.987 2.816 -0.567
ENSG00000099995 E032 545.2642711 8.848972e-03 2.857945e-06 2.149125e-05 22 30356730 30356919 190 - 2.847 2.625 -0.738