ENSG00000099956

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000407082 ENSG00000099956 HEK293_OSMI2_2hA HEK293_TMG_2hB SMARCB1 protein_coding protein_coding 209.7813 334.1333 152.7446 19.09978 1.144132 -1.129251 23.24793 31.48940 18.21797 7.580013 1.0512082 -0.7891704 0.1130208 0.09236667 0.1192000 0.02683333 0.575993808 2.503948e-32 FALSE  
ENST00000407422 ENSG00000099956 HEK293_OSMI2_2hA HEK293_TMG_2hB SMARCB1 protein_coding protein_coding 209.7813 334.1333 152.7446 19.09978 1.144132 -1.129251 36.12616 67.03456 22.73082 1.158435 0.5585487 -1.5598363 0.1641875 0.20153333 0.1489000 -0.05263333 0.002044415 2.503948e-32 FALSE  
ENST00000417137 ENSG00000099956 HEK293_OSMI2_2hA HEK293_TMG_2hB SMARCB1 protein_coding protein_coding 209.7813 334.1333 152.7446 19.09978 1.144132 -1.129251 83.46141 135.60244 62.33567 11.008830 1.7296756 -1.1211283 0.3946208 0.40480000 0.4083000 0.00350000 0.982062383 2.503948e-32 FALSE  
ENST00000646911 ENSG00000099956 HEK293_OSMI2_2hA HEK293_TMG_2hB SMARCB1 protein_coding retained_intron 209.7813 334.1333 152.7446 19.09978 1.144132 -1.129251 49.75510 84.07137 36.13371 2.356876 1.4731374 -1.2180414 0.2373167 0.25296667 0.2364333 -0.01653333 0.755714812 2.503948e-32 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099956 E001 0.8073459 1.603156e-02 1.268113e-01 2.297118e-01 22 23786931 23786962 32 + 0.411 0.147 -1.970
ENSG00000099956 E002 1.2878147 9.600895e-02 5.686650e-02 1.212323e-01 22 23786963 23786965 3 + 0.560 0.206 -2.119
ENSG00000099956 E003 27.9047267 4.341584e-02 5.675414e-02 1.210391e-01 22 23786966 23786977 12 + 1.534 1.331 -0.700
ENSG00000099956 E004 29.3345910 4.783908e-02 6.191208e-02 1.298396e-01 22 23786978 23786979 2 + 1.553 1.353 -0.690
ENSG00000099956 E005 37.4917286 5.090506e-02 2.477287e-02 6.140931e-02 22 23786980 23786982 3 + 1.684 1.443 -0.826
ENSG00000099956 E006 218.0854633 2.778938e-02 1.697949e-02 4.488855e-02 22 23786983 23787000 18 + 2.397 2.223 -0.581
ENSG00000099956 E007 270.8303678 2.826731e-02 1.274649e-02 3.531575e-02 22 23787001 23787010 10 + 2.497 2.314 -0.611
ENSG00000099956 E008 352.0359799 2.866973e-02 2.238745e-02 5.648117e-02 22 23787011 23787016 6 + 2.598 2.433 -0.550
ENSG00000099956 E009 448.2053802 2.181982e-02 2.736114e-02 6.672870e-02 22 23787017 23787026 10 + 2.684 2.546 -0.457
ENSG00000099956 E010 1195.8790789 8.880967e-03 1.857372e-01 3.084251e-01 22 23787027 23787181 155 + 3.040 2.999 -0.136
ENSG00000099956 E011 938.2856603 4.510684e-03 1.969865e-01 3.224561e-01 22 23787182 23787201 20 + 2.924 2.897 -0.088
ENSG00000099956 E012 1250.0996243 3.281886e-03 2.256472e-02 5.685355e-02 22 23787202 23787257 56 + 3.066 3.016 -0.165
ENSG00000099956 E013 742.5649953 1.628523e-03 3.091968e-04 1.416908e-03 22 23787258 23787262 5 + 2.856 2.785 -0.236
ENSG00000099956 E014 1290.3342397 7.797410e-04 1.244289e-04 6.341177e-04 22 23791756 23791800 45 + 3.079 3.029 -0.166
ENSG00000099956 E015 1137.7298243 2.895881e-04 3.683895e-05 2.141595e-04 22 23791801 23791810 10 + 3.019 2.976 -0.142
ENSG00000099956 E016 1608.6906837 8.700129e-05 2.102696e-05 1.297640e-04 22 23791811 23791867 57 + 3.160 3.130 -0.099
ENSG00000099956 E017 681.9092211 6.201179e-04 7.329136e-01 8.242674e-01 22 23791868 23791894 27 + 2.759 2.769 0.032
ENSG00000099956 E018 130.8381171 2.669584e-04 4.164032e-12 9.224315e-11 22 23791895 23793558 1664 + 2.204 1.988 -0.724
ENSG00000099956 E019 2669.9379025 2.698103e-04 2.990427e-04 1.375735e-03 22 23793559 23793688 130 + 3.375 3.353 -0.074
ENSG00000099956 E020 5.7057287 4.766398e-02 9.690135e-01 9.846264e-01 22 23795976 23796028 53 + 0.759 0.778 0.078
ENSG00000099956 E021 1566.4209148 2.089330e-04 1.799930e-01 3.011732e-01 22 23800944 23800970 27 + 3.127 3.127 -0.001
ENSG00000099956 E022 2553.9162932 2.432896e-04 9.772631e-01 9.898412e-01 22 23800971 23801081 111 + 3.327 3.343 0.054
ENSG00000099956 E023 60.6591623 4.093618e-04 4.570120e-03 1.473820e-02 22 23801082 23801135 54 + 1.814 1.689 -0.422
ENSG00000099956 E024 22.9739393 9.922699e-04 6.370767e-18 3.197146e-16 22 23801136 23801298 163 + 1.680 1.074 -2.107
ENSG00000099956 E025 67.5843426 3.577556e-04 5.867255e-49 3.337939e-46 22 23801299 23801964 666 + 2.143 1.524 -2.089
ENSG00000099956 E026 14.6801021 1.228098e-03 9.155870e-11 1.626702e-09 22 23801965 23802000 36 + 1.476 0.927 -1.959
ENSG00000099956 E027 46.2066230 2.446073e-03 4.767989e-23 4.242749e-21 22 23802001 23802546 546 + 1.944 1.404 -1.833
ENSG00000099956 E028 79.2030371 1.533192e-02 1.743698e-11 3.488261e-10 22 23802547 23803294 748 + 2.136 1.667 -1.581
ENSG00000099956 E029 2937.5775137 5.346536e-05 2.719318e-02 6.638573e-02 22 23803295 23803422 128 + 3.374 3.407 0.110
ENSG00000099956 E030 5.8161063 9.351072e-02 4.169821e-01 5.603707e-01 22 23803423 23804383 961 + 0.894 0.743 -0.594
ENSG00000099956 E031 10.5654413 1.623218e-03 1.720744e-01 2.909012e-01 22 23814991 23816624 1634 + 1.100 0.966 -0.488
ENSG00000099956 E032 12.9154020 1.528390e-03 3.446382e-01 4.887246e-01 22 23816625 23816769 145 + 1.134 1.052 -0.298
ENSG00000099956 E033 3808.0219390 3.893211e-04 3.939578e-04 1.751440e-03 22 23816770 23816936 167 + 3.470 3.525 0.182
ENSG00000099956 E034 4.2404269 5.894834e-03 1.684260e-01 2.861327e-01 22 23816937 23818338 1402 + 0.797 0.604 -0.800
ENSG00000099956 E035 2.1369752 2.956744e-02 6.503080e-01 7.613659e-01 22 23818339 23818669 331 + 0.492 0.418 -0.379
ENSG00000099956 E036 0.8878848 1.876930e-01 5.968794e-01 7.187296e-01 22 23822072 23822125 54 + 0.313 0.208 -0.783
ENSG00000099956 E037 21.5817840 9.158074e-04 9.219751e-01 9.547701e-01 22 23823108 23825224 2117 + 1.276 1.299 0.080
ENSG00000099956 E038 4260.3419482 1.423455e-03 9.108132e-05 4.801481e-04 22 23825225 23825415 191 + 3.494 3.581 0.287
ENSG00000099956 E039 18.3688700 7.640577e-03 2.926104e-02 7.050808e-02 22 23825416 23826248 833 + 1.354 1.167 -0.655
ENSG00000099956 E040 3565.8469958 1.790778e-03 2.952711e-06 2.212396e-05 22 23833572 23833703 132 + 3.396 3.508 0.373
ENSG00000099956 E041 2323.3851198 1.964966e-03 1.233326e-12 2.982512e-11 22 23834141 23838009 3869 + 3.156 3.335 0.594