ENSG00000099954

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355219 ENSG00000099954 HEK293_OSMI2_2hA HEK293_TMG_2hB CECR2 protein_coding nonsense_mediated_decay 4.385916 3.604946 4.013094 0.1514191 0.1879042 0.1543311 1.0933867 1.6041638 0.2785823 0.23445489 0.2785823 -2.483732 0.25562083 0.4505333 0.06423333 -0.3863000 5.540233e-02 1.302458e-06 TRUE TRUE
ENST00000612582 ENSG00000099954 HEK293_OSMI2_2hA HEK293_TMG_2hB CECR2 protein_coding protein_coding 4.385916 3.604946 4.013094 0.1514191 0.1879042 0.1543311 1.1328131 0.6045909 1.5080712 0.35809477 0.3317967 1.304541 0.24739167 0.1609667 0.37786667 0.2169000 2.713158e-01 1.302458e-06 FALSE TRUE
MSTRG.21561.5 ENSG00000099954 HEK293_OSMI2_2hA HEK293_TMG_2hB CECR2 protein_coding   4.385916 3.604946 4.013094 0.1514191 0.1879042 0.1543311 0.3863704 0.0000000 0.6597108 0.00000000 0.1651785 6.065466 0.08480833 0.0000000 0.16786667 0.1678667 1.302458e-06 1.302458e-06 FALSE TRUE
MSTRG.21561.6 ENSG00000099954 HEK293_OSMI2_2hA HEK293_TMG_2hB CECR2 protein_coding   4.385916 3.604946 4.013094 0.1514191 0.1879042 0.1543311 0.2970497 0.0000000 0.5381554 0.00000000 0.2737230 5.776513 0.06638333 0.0000000 0.12850000 0.1285000 1.900961e-01 1.302458e-06 FALSE TRUE
MSTRG.21561.7 ENSG00000099954 HEK293_OSMI2_2hA HEK293_TMG_2hB CECR2 protein_coding   4.385916 3.604946 4.013094 0.1514191 0.1879042 0.1543311 1.1418188 1.3961917 0.7026513 0.01682226 0.1354493 -0.980525 0.26806667 0.3885000 0.17766667 -0.2108333 1.115888e-02 1.302458e-06 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099954 E001 0.0000000       22 17359949 17360023 75 +      
ENSG00000099954 E002 0.2987644 0.0270208939 2.282281e-01   22 17369460 17369669 210 + 0.196 0.000 -12.807
ENSG00000099954 E003 0.5932625 0.1336338362 6.573631e-02 1.362652e-01 22 17369670 17369723 54 + 0.331 0.000 -13.808
ENSG00000099954 E004 0.5932625 0.1336338362 6.573631e-02 1.362652e-01 22 17369724 17369730 7 + 0.331 0.000 -13.808
ENSG00000099954 E005 4.9572972 0.0034569835 3.841543e-02 8.812895e-02 22 17369731 17369874 144 + 0.862 0.638 -0.908
ENSG00000099954 E006 7.6973314 0.0030926715 1.122696e-03 4.379430e-03 22 17369875 17369909 35 + 1.063 0.737 -1.243
ENSG00000099954 E007 0.0000000       22 17428273 17428540 268 +      
ENSG00000099954 E008 0.0000000       22 17477093 17477159 67 +      
ENSG00000099954 E009 14.4617386 0.0074535565 1.423429e-05 9.124768e-05 22 17477588 17477682 95 + 1.327 0.962 -1.310
ENSG00000099954 E010 28.1695906 0.0103074764 2.901283e-04 1.339799e-03 22 17497403 17497586 184 + 1.557 1.332 -0.778
ENSG00000099954 E011 25.7653486 0.0036432585 7.422832e-02 1.502996e-01 22 17499410 17499549 140 + 1.453 1.394 -0.203
ENSG00000099954 E012 20.2273301 0.0014396770 3.051674e-03 1.041789e-02 22 17500631 17500735 105 + 1.397 1.239 -0.552
ENSG00000099954 E013 16.2974492 0.0092394133 1.284946e-01 2.320627e-01 22 17503082 17503131 50 + 1.271 1.200 -0.250
ENSG00000099954 E014 29.7725804 0.0007161676 2.348653e-02 5.874873e-02 22 17504847 17505016 170 + 1.521 1.449 -0.249
ENSG00000099954 E015 1.4350506 0.0095790160 3.940761e-01 5.379716e-01 22 17511813 17511896 84 + 0.433 0.325 -0.618
ENSG00000099954 E016 29.9223732 0.0006469680 8.595408e-03 2.527747e-02 22 17524118 17524271 154 + 1.532 1.437 -0.327
ENSG00000099954 E017 0.0000000       22 17524272 17524327 56 +      
ENSG00000099954 E018 0.0000000       22 17534744 17534747 4 +      
ENSG00000099954 E019 23.8125038 0.0008432299 7.315553e-02 1.485657e-01 22 17537103 17537232 130 + 1.417 1.360 -0.195
ENSG00000099954 E020 13.1646023 0.0013411664 2.165443e-02 5.496057e-02 22 17538520 17538557 38 + 1.215 1.071 -0.516
ENSG00000099954 E021 20.1183002 0.0009737901 7.185933e-04 2.965460e-03 22 17538640 17538731 92 + 1.402 1.210 -0.670
ENSG00000099954 E022 24.3467777 0.0398922763 2.154949e-02 5.474556e-02 22 17538993 17539119 127 + 1.478 1.303 -0.607
ENSG00000099954 E023 32.8379056 0.0202514886 6.025899e-03 1.867966e-02 22 17540412 17540716 305 + 1.599 1.430 -0.580
ENSG00000099954 E024 14.5407793 0.0014680010 1.557495e-01 2.694334e-01 22 17540717 17540800 84 + 1.215 1.158 -0.203
ENSG00000099954 E025 17.0769499 0.0026666495 1.073604e-03 4.213439e-03 22 17541839 17541967 129 + 1.344 1.135 -0.740
ENSG00000099954 E026 44.2055320 0.0227130794 2.206798e-01 3.512272e-01 22 17542157 17542601 445 + 1.671 1.638 -0.110
ENSG00000099954 E027 27.6941858 0.0208670889 3.817227e-01 5.260394e-01 22 17542602 17542838 237 + 1.461 1.447 -0.047
ENSG00000099954 E028 20.5368810 0.0015540585 9.378403e-01 9.649427e-01 22 17542839 17543003 165 + 1.303 1.367 0.222
ENSG00000099954 E029 10.4202763 0.0030352819 8.004231e-01 8.727836e-01 22 17548148 17548150 3 + 1.017 1.110 0.335
ENSG00000099954 E030 13.6644722 0.0018478652 3.429264e-01 4.869642e-01 22 17548151 17548189 39 + 1.175 1.157 -0.064
ENSG00000099954 E031 69.8147980 0.0011584285 9.418102e-03 2.732653e-02 22 17548190 17548998 809 + 1.866 1.825 -0.138
ENSG00000099954 E032 11.2380449 0.0056336470 2.718896e-01 4.106959e-01 22 17548999 17549002 4 + 1.104 1.057 -0.168
ENSG00000099954 E033 62.8500654 0.0003960472 6.571489e-02 1.362297e-01 22 17549003 17549564 562 + 1.808 1.800 -0.030
ENSG00000099954 E034 0.0000000       22 17550243 17550409 167 +      
ENSG00000099954 E035 25.8831403 0.0067319753 9.976185e-01 1.000000e+00 22 17552031 17552142 112 + 1.392 1.461 0.236
ENSG00000099954 E036 56.1858932 0.0004975430 1.575866e-01 2.719096e-01 22 17552835 17553227 393 + 1.748 1.754 0.018
ENSG00000099954 E037 43.4984582 0.0005386158 4.647424e-01 6.044610e-01 22 17553228 17553518 291 + 1.590 1.699 0.371
ENSG00000099954 E038 23.8250964 0.0008677443 2.504295e-02 6.196369e-02 22 17553519 17553564 46 + 1.265 1.493 0.791
ENSG00000099954 E039 102.4659393 0.0045615260 2.799111e-01 4.197038e-01 22 17553565 17554641 1077 + 1.946 2.069 0.415
ENSG00000099954 E040 64.3042520 0.0004430104 6.821812e-01 7.856698e-01 22 17554642 17555283 642 + 1.766 1.855 0.301
ENSG00000099954 E041 289.5687766 0.0184187922 6.514618e-09 8.318784e-08 22 17555284 17558151 2868 + 2.229 2.624 1.317