ENSG00000099889

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263207 ENSG00000099889 HEK293_OSMI2_2hA HEK293_TMG_2hB ARVCF protein_coding protein_coding 10.33859 12.66596 6.222294 0.7381712 0.2815391 -1.02426 0.6596958 0.8489466 0.11893586 0.53341234 0.05963381 -2.7359149 0.06273333 0.06850000 0.018300000 -0.05020000 0.82938488 0.03935828 FALSE TRUE
ENST00000406259 ENSG00000099889 HEK293_OSMI2_2hA HEK293_TMG_2hB ARVCF protein_coding protein_coding 10.33859 12.66596 6.222294 0.7381712 0.2815391 -1.02426 0.2288755 0.0000000 0.35269848 0.00000000 0.35269848 5.1806988 0.03016667 0.00000000 0.053666667 0.05366667 0.72566014 0.03935828 FALSE TRUE
ENST00000406522 ENSG00000099889 HEK293_OSMI2_2hA HEK293_TMG_2hB ARVCF protein_coding protein_coding 10.33859 12.66596 6.222294 0.7381712 0.2815391 -1.02426 0.9930273 1.7794358 1.20109890 0.84637035 0.60061043 -0.5631881 0.10211667 0.13423333 0.199266667 0.06503333 0.99165173 0.03935828 FALSE TRUE
ENST00000473551 ENSG00000099889 HEK293_OSMI2_2hA HEK293_TMG_2hB ARVCF protein_coding retained_intron 10.33859 12.66596 6.222294 0.7381712 0.2815391 -1.02426 1.1059595 0.8598688 0.76091612 0.08606984 0.10090565 -0.1742239 0.11442083 0.06770000 0.121300000 0.05360000 0.03935828 0.03935828 FALSE TRUE
ENST00000495096 ENSG00000099889 HEK293_OSMI2_2hA HEK293_TMG_2hB ARVCF protein_coding retained_intron 10.33859 12.66596 6.222294 0.7381712 0.2815391 -1.02426 2.7501753 3.7648315 1.62023313 0.34960888 0.13928607 -1.2113340 0.26253333 0.29823333 0.260000000 -0.03823333 0.71595174 0.03935828 FALSE TRUE
MSTRG.21649.2 ENSG00000099889 HEK293_OSMI2_2hA HEK293_TMG_2hB ARVCF protein_coding   10.33859 12.66596 6.222294 0.7381712 0.2815391 -1.02426 2.0109408 3.4488462 1.14621224 0.81580578 0.21184204 -1.5808846 0.18492917 0.26823333 0.184300000 -0.08393333 0.57478483 0.03935828 FALSE TRUE
MSTRG.21649.5 ENSG00000099889 HEK293_OSMI2_2hA HEK293_TMG_2hB ARVCF protein_coding   10.33859 12.66596 6.222294 0.7381712 0.2815391 -1.02426 1.3188261 1.0411382 0.04826864 0.59403545 0.04826864 -4.1730888 0.11525000 0.08753333 0.007333333 -0.08020000 0.48902533 0.03935828 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099889 E001 0.4448795 0.5325157125 6.326545e-01 7.474695e-01 22 19969896 19969897 2 - 0.001 0.194 8.724
ENSG00000099889 E002 45.3647516 0.0005523980 1.364940e-05 8.786404e-05 22 19969898 19970061 164 - 1.358 1.646 0.990
ENSG00000099889 E003 109.8440094 0.0042148079 4.664101e-04 2.031638e-03 22 19970062 19970367 306 - 1.832 2.011 0.601
ENSG00000099889 E004 94.4457117 0.0014517921 6.420293e-04 2.687373e-03 22 19970368 19970743 376 - 1.786 1.943 0.529
ENSG00000099889 E005 10.5408315 0.0189228587 3.620558e-02 8.397555e-02 22 19971216 19971227 12 - 0.762 1.055 1.114
ENSG00000099889 E006 19.1238041 0.0077176535 1.298902e-01 2.340118e-01 22 19971228 19971335 108 - 1.121 1.272 0.535
ENSG00000099889 E007 73.8369586 0.0003950152 2.304460e-01 3.629920e-01 22 19971886 19971971 86 - 1.758 1.813 0.185
ENSG00000099889 E008 59.8414722 0.0004807976 1.074297e-01 2.017529e-01 22 19972358 19972411 54 - 1.649 1.733 0.283
ENSG00000099889 E009 76.8282411 0.0003923748 8.307308e-02 1.643633e-01 22 19972737 19972827 91 - 1.758 1.837 0.267
ENSG00000099889 E010 79.5835873 0.0003560726 3.572462e-01 5.016434e-01 22 19972925 19973036 112 - 1.804 1.844 0.133
ENSG00000099889 E011 43.0642715 0.0005176308 7.860014e-01 8.629508e-01 22 19973119 19973161 43 - 1.557 1.572 0.050
ENSG00000099889 E012 75.5826030 0.0003749068 7.332390e-01 8.244793e-01 22 19973162 19973317 156 - 1.800 1.814 0.046
ENSG00000099889 E013 99.6297834 0.0002922335 4.947224e-01 6.316517e-01 22 19973643 19973793 151 - 1.910 1.935 0.086
ENSG00000099889 E014 87.6527404 0.0003747586 5.950943e-01 7.172319e-01 22 19974112 19974239 128 - 1.859 1.880 0.071
ENSG00000099889 E015 1.8561712 0.1889155431 5.906874e-02 1.250296e-01 22 19974240 19974278 39 - 0.675 0.259 -2.190
ENSG00000099889 E016 67.2588021 0.0015509471 9.169886e-01 9.515713e-01 22 19975686 19975757 72 - 1.758 1.763 0.014
ENSG00000099889 E017 0.9492964 0.0148287830 2.473777e-01 3.828586e-01 22 19975758 19975762 5 - 0.414 0.203 -1.415
ENSG00000099889 E018 0.6966155 0.0166989808 9.327979e-01 9.616557e-01 22 19976706 19976723 18 - 0.185 0.204 0.171
ENSG00000099889 E019 78.9426563 0.0020836378 6.242041e-01 7.406079e-01 22 19977415 19977586 172 - 1.845 1.821 -0.082
ENSG00000099889 E020 72.0858105 0.0005468446 6.762618e-01 7.812843e-01 22 19977958 19978075 118 - 1.804 1.783 -0.072
ENSG00000099889 E021 95.4901869 0.0010507369 1.134929e-01 2.106217e-01 22 19978897 19979080 184 - 1.961 1.893 -0.226
ENSG00000099889 E022 15.7803688 0.0015612386 5.216988e-07 4.598012e-06 22 19979081 19979416 336 - 1.440 1.007 -1.535
ENSG00000099889 E023 80.8370673 0.0029848785 6.319603e-02 1.320295e-01 22 19979743 19979996 254 - 1.904 1.812 -0.310
ENSG00000099889 E024 68.2035109 0.0234537678 6.474473e-01 7.591097e-01 22 19979997 19980242 246 - 1.793 1.758 -0.119
ENSG00000099889 E025 28.6738104 0.0007630270 8.149790e-05 4.350635e-04 22 19980243 19981151 909 - 1.588 1.321 -0.920
ENSG00000099889 E026 5.1230172 0.0032384165 2.472905e-03 8.682505e-03 22 19981152 19981210 59 - 1.001 0.601 -1.596
ENSG00000099889 E027 72.7548811 0.0032186532 2.550416e-01 3.918421e-01 22 19981211 19981381 171 - 1.836 1.776 -0.202
ENSG00000099889 E028 62.3238845 0.0067230434 9.619888e-01 9.800248e-01 22 19981382 19981544 163 - 1.725 1.724 -0.003
ENSG00000099889 E029 63.0119947 0.0004281388 3.479517e-01 4.921635e-01 22 19981545 19981737 193 - 1.698 1.744 0.155
ENSG00000099889 E030 40.1523201 0.0020392722 1.899061e-02 4.931213e-02 22 19981933 19981990 58 - 1.649 1.502 -0.503
ENSG00000099889 E031 45.4774677 0.0005209704 5.149591e-04 2.215352e-03 22 19981991 19982091 101 - 1.733 1.540 -0.657
ENSG00000099889 E032 1.2813702 0.0576680132 5.570967e-01 6.855893e-01 22 19983673 19983753 81 - 0.414 0.299 -0.683
ENSG00000099889 E033 1.1447053 0.0181599846 1.058095e-01 1.993257e-01 22 19986651 19986683 33 - 0.495 0.204 -1.829
ENSG00000099889 E034 5.5048599 0.0741306022 1.252020e-01 2.274279e-01 22 19987073 19987190 118 - 0.952 0.677 -1.082
ENSG00000099889 E035 30.8973235 0.0280027222 2.503955e-01 3.864085e-01 22 19990585 19990812 228 - 1.532 1.400 -0.454
ENSG00000099889 E036 14.3817749 0.0203639618 6.005208e-01 7.217427e-01 22 20010455 20010508 54 - 1.171 1.105 -0.235
ENSG00000099889 E037 14.3809488 0.0013661218 2.905170e-02 7.010620e-02 22 20016589 20016823 235 - 1.268 1.063 -0.731