ENSG00000099875

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000250896 ENSG00000099875 HEK293_OSMI2_2hA HEK293_TMG_2hB MKNK2 protein_coding protein_coding 84.15401 132.6125 51.15036 9.932023 1.52106 -1.374227 39.58806 71.187749 19.477645 4.7970667 0.2492829 -1.8692719 0.4467750 0.53750000 0.3812000 -0.15630000 5.047783e-15 2.903061e-19 FALSE TRUE
ENST00000309340 ENSG00000099875 HEK293_OSMI2_2hA HEK293_TMG_2hB MKNK2 protein_coding protein_coding 84.15401 132.6125 51.15036 9.932023 1.52106 -1.374227 11.70979 8.045077 12.602311 1.4220355 0.3991577 0.6468623 0.1675833 0.05976667 0.2464000 0.18663333 2.903061e-19 2.903061e-19 FALSE TRUE
ENST00000589509 ENSG00000099875 HEK293_OSMI2_2hA HEK293_TMG_2hB MKNK2 protein_coding protein_coding 84.15401 132.6125 51.15036 9.932023 1.52106 -1.374227 13.28764 18.611101 9.073665 3.2479654 1.2855930 -1.0355919 0.1605375 0.13816667 0.1763333 0.03816667 5.181143e-01 2.903061e-19 FALSE FALSE
ENST00000591142 ENSG00000099875 HEK293_OSMI2_2hA HEK293_TMG_2hB MKNK2 protein_coding protein_coding 84.15401 132.6125 51.15036 9.932023 1.52106 -1.374227 13.22519 29.038454 5.176029 0.8941298 0.4752808 -2.4857593 0.1370083 0.22213333 0.1018667 -0.12026667 7.541267e-04 2.903061e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099875 E001 0.000000       19 2037465 2037470 6 -      
ENSG00000099875 E002 2.835564 0.0060010608 5.999522e-02 1.265932e-01 19 2037471 2037471 1 - 0.195 0.581 2.309
ENSG00000099875 E003 4.603730 0.0046150864 3.064598e-02 7.322497e-02 19 2037472 2037472 1 - 0.329 0.740 1.988
ENSG00000099875 E004 1025.709431 0.0075783234 2.402551e-04 1.134317e-03 19 2037473 2037829 357 - 2.776 2.959 0.608
ENSG00000099875 E005 1000.926341 0.0033045205 2.847790e-12 6.488338e-11 19 2037830 2038247 418 - 2.718 2.956 0.791
ENSG00000099875 E006 1804.920951 0.0001354012 2.320019e-19 1.381706e-17 19 2038248 2039411 1164 - 3.070 3.190 0.398
ENSG00000099875 E007 471.922453 0.0004941221 1.071904e-04 5.552124e-04 19 2039412 2039630 219 - 2.495 2.605 0.365
ENSG00000099875 E008 515.616472 0.0016390925 1.176222e-01 2.165517e-01 19 2039631 2039856 226 - 2.572 2.638 0.222
ENSG00000099875 E009 19.920629 0.0020240132 4.163840e-01 5.597455e-01 19 2039857 2039954 98 - 1.268 1.214 -0.190
ENSG00000099875 E010 31.492459 0.0085020325 1.912905e-01 3.153474e-01 19 2039955 2040131 177 - 1.483 1.399 -0.292
ENSG00000099875 E011 16.999483 0.0107325775 8.823499e-02 1.724255e-01 19 2040132 2040133 2 - 1.281 1.129 -0.542
ENSG00000099875 E012 463.532118 0.0001994532 5.056725e-01 6.414080e-01 19 2040134 2040177 44 - 2.549 2.583 0.114
ENSG00000099875 E013 7.935765 0.0023791164 1.266616e-01 2.294940e-01 19 2040178 2040308 131 - 1.002 0.833 -0.643
ENSG00000099875 E014 18.794369 0.0011493800 1.722591e-10 2.919817e-09 19 2040309 2041039 731 - 1.544 1.053 -1.722
ENSG00000099875 E015 787.517452 0.0001406376 2.873631e-01 4.278637e-01 19 2041040 2041204 165 - 2.802 2.807 0.014
ENSG00000099875 E016 791.136393 0.0003796525 5.217659e-03 1.652296e-02 19 2041840 2042034 195 - 2.829 2.804 -0.084
ENSG00000099875 E017 29.441547 0.0018476451 5.486334e-04 2.341977e-03 19 2042035 2042140 106 - 1.564 1.338 -0.778
ENSG00000099875 E018 566.743490 0.0001055555 4.087217e-04 1.807874e-03 19 2042427 2042522 96 - 2.696 2.654 -0.139
ENSG00000099875 E019 555.709789 0.0001378978 3.757373e-02 8.653542e-02 19 2042607 2042662 56 - 2.668 2.651 -0.058
ENSG00000099875 E020 13.643428 0.0245256472 1.041388e-06 8.606923e-06 19 2042663 2042765 103 - 1.432 0.893 -1.935
ENSG00000099875 E021 562.743109 0.0001480647 1.057943e-01 1.993041e-01 19 2042766 2042870 105 - 2.669 2.660 -0.030
ENSG00000099875 E022 509.739703 0.0005576538 1.438804e-03 5.435778e-03 19 2043124 2043197 74 - 2.656 2.611 -0.152
ENSG00000099875 E023 589.431854 0.0001495420 3.279661e-11 6.275345e-10 19 2043503 2043582 80 - 2.753 2.660 -0.310
ENSG00000099875 E024 526.881166 0.0003131143 1.049671e-11 2.178300e-10 19 2046186 2046283 98 - 2.715 2.607 -0.359
ENSG00000099875 E025 477.315391 0.0024802619 5.793046e-06 4.064615e-05 19 2046367 2046460 94 - 2.675 2.566 -0.365
ENSG00000099875 E026 240.411969 0.0024604636 3.615463e-05 2.105883e-04 19 2046461 2046468 8 - 2.384 2.264 -0.401
ENSG00000099875 E027 121.019938 0.0003030915 4.013018e-03 1.317837e-02 19 2046522 2046603 82 - 2.062 1.974 -0.293
ENSG00000099875 E028 294.173038 0.0019426178 4.439790e-07 3.967289e-06 19 2046604 2046691 88 - 2.482 2.348 -0.447
ENSG00000099875 E029 171.058465 0.0002190494 7.954805e-07 6.749133e-06 19 2050801 2050947 147 - 2.245 2.113 -0.443
ENSG00000099875 E030 13.576328 0.0405401418 4.840119e-04 2.098899e-03 19 2051096 2051217 122 - 1.374 0.944 -1.542
ENSG00000099875 E031 5.014681 0.0062916616 5.824808e-07 5.089440e-06 19 2051218 2051244 27 - 1.110 0.458 -2.665