ENSG00000099864

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264560 ENSG00000099864 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM protein_coding protein_coding 13.91652 24.98745 8.579278 1.958773 1.440609 -1.541172 3.791383 7.936222 2.8229189 0.8378396 0.6851892 -1.487980 0.3223458 0.3215000 0.38560000 0.06410000 0.955362149 0.007731396 FALSE  
ENST00000338448 ENSG00000099864 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM protein_coding protein_coding 13.91652 24.98745 8.579278 1.958773 1.440609 -1.541172 1.387664 2.555918 1.1272445 0.2795287 0.2560359 -1.173933 0.1016500 0.1050333 0.14263333 0.03760000 0.822167187 0.007731396 FALSE  
ENST00000589012 ENSG00000099864 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM protein_coding retained_intron 13.91652 24.98745 8.579278 1.958773 1.440609 -1.541172 2.387269 1.474315 2.5535355 1.4743146 1.3293357 0.788338 0.1790875 0.0511000 0.25410000 0.20300000 0.384703589 0.007731396 FALSE  
ENST00000592870 ENSG00000099864 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM protein_coding retained_intron 13.91652 24.98745 8.579278 1.958773 1.440609 -1.541172 1.519890 3.184112 0.5819322 0.1563708 0.1166557 -2.431911 0.1112792 0.1284333 0.06696667 -0.06146667 0.007731396 0.007731396 FALSE  
ENST00000633534 ENSG00000099864 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM protein_coding retained_intron 13.91652 24.98745 8.579278 1.958773 1.440609 -1.541172 4.736245 9.808508 1.4936465 0.9583399 0.7581090 -2.707038 0.2760417 0.3927000 0.15070000 -0.24200000 0.440884782 0.007731396 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099864 E001 0.2998086 0.0293173952 3.439058e-01   19 708935 708938 4 + 0.204 0.071 -1.759
ENSG00000099864 E002 2.4398040 0.0186574478 6.323577e-01 0.7472306559 19 708939 709096 158 + 0.531 0.468 -0.303
ENSG00000099864 E003 16.0047290 0.0024352539 3.047606e-01 0.4467864021 19 709097 709130 34 + 1.186 1.116 -0.252
ENSG00000099864 E004 23.4094991 0.0008297310 1.835268e-01 0.3056312471 19 709131 709151 21 + 1.353 1.276 -0.268
ENSG00000099864 E005 0.3268771 0.0291667019 3.459543e-01   19 719100 719657 558 + 0.204 0.071 -1.755
ENSG00000099864 E006 0.1515154 0.0438358305 1.209531e-01   19 722504 722907 404 + 0.204 0.000 -12.844
ENSG00000099864 E007 48.1656241 0.0005332682 5.856786e-02 0.1241728034 19 726138 726189 52 + 1.650 1.572 -0.266
ENSG00000099864 E008 80.1085490 0.0003438325 4.880298e-02 0.1070325512 19 727008 727088 81 + 1.851 1.794 -0.191
ENSG00000099864 E009 122.1556333 0.0003259862 1.854739e-03 0.0067723154 19 727564 727694 131 + 2.050 1.970 -0.269
ENSG00000099864 E010 0.0000000       19 727786 727864 79 +      
ENSG00000099864 E011 0.4031496 0.0248994611 1.000000e+00 1.0000000000 19 731092 731094 3 + 0.000 0.132 11.146
ENSG00000099864 E012 139.0793560 0.0002473038 2.861087e-03 0.0098562413 19 731095 731245 151 + 2.098 2.031 -0.227
ENSG00000099864 E013 73.6278198 0.0077083742 8.521612e-01 0.9083662519 19 734173 734194 22 + 1.753 1.775 0.075
ENSG00000099864 E014 43.3918737 0.0008395184 3.247909e-03 0.0109952759 19 734195 735029 835 + 1.341 1.588 0.849
ENSG00000099864 E015 0.5514428 0.0197023815 5.418938e-01 0.6727754938 19 735030 735032 3 + 0.000 0.185 11.774
ENSG00000099864 E016 0.5514428 0.0197023815 5.418938e-01 0.6727754938 19 735033 735054 22 + 0.000 0.185 11.774
ENSG00000099864 E017 0.4031496 0.0248994611 1.000000e+00 1.0000000000 19 735055 735064 10 + 0.000 0.132 11.146
ENSG00000099864 E018 0.2214452 0.0389186046 1.000000e+00   19 735065 735110 46 + 0.000 0.071 10.037
ENSG00000099864 E019 79.3897639 0.0026523974 3.741159e-02 0.0862437761 19 736019 736078 60 + 1.862 1.790 -0.242
ENSG00000099864 E020 0.2944980 0.3448214699 5.271698e-02   19 736079 736139 61 + 0.341 0.000 -13.071
ENSG00000099864 E021 17.6004821 0.0013131387 1.911441e-01 0.3151657914 19 740352 740483 132 + 1.249 1.160 -0.316
ENSG00000099864 E022 798.0263812 0.0030079853 3.518521e-05 0.0002056159 19 746285 748329 2045 + 2.714 2.822 0.360