ENSG00000099840

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395296 ENSG00000099840 HEK293_OSMI2_2hA HEK293_TMG_2hB IZUMO4 protein_coding protein_coding 3.335623 5.045774 2.180199 0.4137876 0.2817695 -1.20687 0.8462098 2.26281074 0.6646586 0.12526435 0.14758331 -1.75224804 0.2311708 0.452033333 0.2982000 -0.153833333 1.205399e-01 1.391797e-05 FALSE TRUE
ENST00000478879 ENSG00000099840 HEK293_OSMI2_2hA HEK293_TMG_2hB IZUMO4 protein_coding retained_intron 3.335623 5.045774 2.180199 0.4137876 0.2817695 -1.20687 0.5780987 0.77203219 0.2129628 0.06701920 0.11041914 -1.81042520 0.1713458 0.152933333 0.1062667 -0.046666667 6.969454e-01 1.391797e-05 TRUE TRUE
ENST00000481489 ENSG00000099840 HEK293_OSMI2_2hA HEK293_TMG_2hB IZUMO4 protein_coding retained_intron 3.335623 5.045774 2.180199 0.4137876 0.2817695 -1.20687 0.1792023 0.12650106 0.2338655 0.02244854 0.09803319 0.83717327 0.0553500 0.026000000 0.1004000 0.074400000 7.925720e-02 1.391797e-05 FALSE FALSE
ENST00000497445 ENSG00000099840 HEK293_OSMI2_2hA HEK293_TMG_2hB IZUMO4 protein_coding retained_intron 3.335623 5.045774 2.180199 0.4137876 0.2817695 -1.20687 0.3178832 0.03801734 0.2337363 0.02105722 0.04091211 2.34369375 0.1064458 0.007066667 0.1072333 0.100166667 1.391797e-05 1.391797e-05 TRUE TRUE
ENST00000590985 ENSG00000099840 HEK293_OSMI2_2hA HEK293_TMG_2hB IZUMO4 protein_coding retained_intron 3.335623 5.045774 2.180199 0.4137876 0.2817695 -1.20687 0.4902653 0.48930416 0.4819370 0.25625203 0.16812778 -0.02144525 0.1589458 0.091866667 0.2258000 0.133933333 5.507781e-01 1.391797e-05 FALSE TRUE
ENST00000620263 ENSG00000099840 HEK293_OSMI2_2hA HEK293_TMG_2hB IZUMO4 protein_coding protein_coding 3.335623 5.045774 2.180199 0.4137876 0.2817695 -1.20687 0.2563849 0.58796751 0.2359201 0.21648040 0.04472699 -1.28187750 0.0764750 0.113633333 0.1080000 -0.005633333 9.877915e-01 1.391797e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099840 E001 3.3203812 0.0047699205 0.0000611941 0.000337360 19 2096429 2096858 430 + 0.942 0.348 -2.658
ENSG00000099840 E002 0.2998086 0.0281526017 0.5045605030   19 2096859 2096881 23 + 0.181 0.081 -1.339
ENSG00000099840 E003 0.4481018 0.1167195645 0.8411951772 0.900935086 19 2096882 2096892 11 + 0.181 0.147 -0.356
ENSG00000099840 E004 1.0382412 0.0125455982 0.8072843887 0.877577197 19 2096893 2096914 22 + 0.308 0.260 -0.336
ENSG00000099840 E005 1.3327393 0.0146342507 0.2149497979 0.344368663 19 2096915 2096930 16 + 0.487 0.260 -1.336
ENSG00000099840 E006 2.1872129 0.0073478694 0.1895173332 0.313143694 19 2096931 2096942 12 + 0.613 0.386 -1.114
ENSG00000099840 E007 8.4808488 0.0038484913 0.5573125705 0.685748024 19 2096943 2096997 55 + 0.858 0.923 0.251
ENSG00000099840 E008 8.9529090 0.0028031374 0.1556722948 0.269317832 19 2096998 2097006 9 + 0.790 0.964 0.666
ENSG00000099840 E009 20.1534104 0.0012915351 0.1432835024 0.252492373 19 2097007 2097086 80 + 1.160 1.280 0.424
ENSG00000099840 E010 22.0063331 0.0047892392 0.4339479642 0.576339146 19 2097087 2097162 76 + 1.244 1.304 0.209
ENSG00000099840 E011 26.8409108 0.0009210936 0.0374965169 0.086397502 19 2097252 2097332 81 + 1.257 1.408 0.527
ENSG00000099840 E012 3.9428058 0.0043984839 0.5253100891 0.658691600 19 2097333 2097423 91 + 0.555 0.652 0.431
ENSG00000099840 E013 29.5533945 0.0006896552 0.3878239799 0.531962807 19 2097424 2097495 72 + 1.376 1.428 0.182
ENSG00000099840 E014 1.8519193 0.0077945242 0.0010362889 0.004083742 19 2097585 2097812 228 + 0.752 0.208 -2.922
ENSG00000099840 E015 19.0966455 0.0012031437 0.0560833324 0.119849963 19 2097929 2097955 27 + 1.356 1.197 -0.559
ENSG00000099840 E016 6.0633141 0.0707900154 0.0717159066 0.146208829 19 2097956 2098051 96 + 0.991 0.705 -1.110
ENSG00000099840 E017 17.1612067 0.0247660517 0.3005234952 0.442220799 19 2098052 2098127 76 + 1.281 1.168 -0.400
ENSG00000099840 E018 8.1102091 0.0022553541 0.3140969087 0.456779896 19 2098128 2098286 159 + 0.991 0.866 -0.469
ENSG00000099840 E019 17.9973882 0.0031587797 0.3655278165 0.509843252 19 2098287 2098334 48 + 1.160 1.235 0.267
ENSG00000099840 E020 14.3701613 0.0029376347 0.5918396488 0.714515396 19 2098436 2098450 15 + 1.175 1.116 -0.210
ENSG00000099840 E021 14.4977968 0.0046272349 0.8882606943 0.932574895 19 2098451 2098786 336 + 1.110 1.118 0.029
ENSG00000099840 E022 7.7664753 0.0026831042 0.1260891963 0.228710588 19 2098787 2098804 18 + 0.711 0.913 0.795
ENSG00000099840 E023 5.7057621 0.0057542776 0.3892656962 0.533371623 19 2098805 2098901 97 + 0.665 0.787 0.503
ENSG00000099840 E024 5.0153055 0.0072764016 0.2384199520 0.372301124 19 2098902 2098975 74 + 0.858 0.689 -0.676
ENSG00000099840 E025 5.4194001 0.0384072452 0.2749138989 0.414141938 19 2098976 2099029 54 + 0.888 0.719 -0.665
ENSG00000099840 E026 10.1801347 0.0025258961 0.3304382605 0.474027435 19 2099030 2099254 225 + 1.074 0.963 -0.408
ENSG00000099840 E027 25.2174895 0.0008211680 0.0381470956 0.087634688 19 2099255 2099593 339 + 1.231 1.387 0.543