ENSG00000099817

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215587 ENSG00000099817 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR2E protein_coding nonsense_mediated_decay 353.7535 657.8337 184.1836 31.68888 4.481463 -1.836522 120.13995 211.05612 50.37551 3.651196 3.3020208 -2.0666143 0.3388375 0.32196667 0.2745000 -0.04746667 4.443841e-01 2.951499e-17 TRUE  
ENST00000589737 ENSG00000099817 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR2E protein_coding nonsense_mediated_decay 353.7535 657.8337 184.1836 31.68888 4.481463 -1.836522 78.64481 166.14905 31.98817 8.532066 2.8537719 -2.3765034 0.2029833 0.25410000 0.1731333 -0.08096667 3.436896e-02 2.951499e-17 FALSE  
ENST00000614705 ENSG00000099817 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR2E protein_coding processed_transcript 353.7535 657.8337 184.1836 31.68888 4.481463 -1.836522 16.39292 21.24641 22.87341 13.052161 4.4572322 0.1064039 0.0623375 0.03056667 0.1234667 0.09290000 5.270068e-01 2.951499e-17 FALSE  
ENST00000615234 ENSG00000099817 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR2E protein_coding protein_coding 353.7535 657.8337 184.1836 31.68888 4.481463 -1.836522 32.48403 51.54498 26.56985 3.201559 0.6087884 -0.9557791 0.1043000 0.07826667 0.1444000 0.06613333 2.951499e-17 2.951499e-17 FALSE  
MSTRG.16038.4 ENSG00000099817 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR2E protein_coding   353.7535 657.8337 184.1836 31.68888 4.481463 -1.836522 90.43974 192.46677 36.90972 14.896889 2.6784420 -2.3822207 0.2312833 0.29183333 0.2012667 -0.09056667 2.425844e-02 2.951499e-17 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099817 E001 122.712269 2.782070e-04 2.459325e-12 5.661139e-11 19 1086574 1086578 5 - 2.164 1.903 -0.876
ENSG00000099817 E002 144.835019 3.148634e-03 5.236521e-08 5.623190e-07 19 1086579 1086594 16 - 2.215 1.982 -0.782
ENSG00000099817 E003 424.739259 2.909533e-04 2.469540e-11 4.826647e-10 19 1086595 1086867 273 - 2.613 2.465 -0.492
ENSG00000099817 E004 350.701080 3.516186e-04 2.680401e-19 1.580928e-17 19 1086868 1086988 121 - 2.582 2.368 -0.713
ENSG00000099817 E005 879.422028 6.324716e-04 1.194073e-58 1.160069e-55 19 1086989 1087644 656 - 3.037 2.748 -0.964
ENSG00000099817 E006 557.634752 3.656315e-03 9.584209e-25 1.022929e-22 19 1087645 1087958 314 - 2.880 2.535 -1.146
ENSG00000099817 E007 313.491069 4.432392e-03 1.381623e-18 7.474140e-17 19 1087959 1088065 107 - 2.631 2.287 -1.148
ENSG00000099817 E008 253.859605 1.305658e-03 9.006501e-40 3.057804e-37 19 1088066 1088147 82 - 2.572 2.180 -1.307
ENSG00000099817 E009 184.573353 2.133115e-03 6.068190e-24 5.993746e-22 19 1088148 1088162 15 - 2.414 2.049 -1.219
ENSG00000099817 E010 162.608902 1.156008e-03 6.491770e-16 2.513995e-14 19 1088163 1088166 4 - 2.302 2.016 -0.956
ENSG00000099817 E011 3211.421648 6.236447e-05 1.000493e-19 6.161914e-18 19 1088167 1088387 221 - 3.300 3.385 0.280
ENSG00000099817 E012 2192.208425 5.038469e-04 1.215632e-20 8.395840e-19 19 1088388 1088423 36 - 3.083 3.228 0.480
ENSG00000099817 E013 4622.771006 7.266651e-04 3.692359e-11 7.000664e-10 19 1088424 1088608 185 - 3.449 3.546 0.324
ENSG00000099817 E014 2509.062501 1.207285e-03 4.371144e-04 1.917209e-03 19 1088609 1088644 36 - 3.206 3.277 0.236
ENSG00000099817 E015 2724.316062 1.009315e-03 2.684033e-03 9.325939e-03 19 1088645 1088681 37 - 3.254 3.311 0.189
ENSG00000099817 E016 2521.855811 4.174116e-04 1.099035e-02 3.114011e-02 19 1088682 1088713 32 - 3.236 3.274 0.125
ENSG00000099817 E017 1942.622026 5.746726e-04 9.501566e-02 1.829069e-01 19 1088714 1088720 7 - 3.129 3.159 0.099
ENSG00000099817 E018 2337.517065 4.329523e-04 1.754814e-04 8.590068e-04 19 1089472 1089489 18 - 3.188 3.243 0.184
ENSG00000099817 E019 2693.722047 1.843684e-04 2.079552e-12 4.844030e-11 19 1089490 1089551 62 - 3.227 3.308 0.270
ENSG00000099817 E020 14.269204 2.824762e-02 1.350213e-01 2.411192e-01 19 1089552 1089573 22 - 1.199 1.014 -0.670
ENSG00000099817 E021 23.189176 2.703961e-03 2.368830e-04 1.120239e-03 19 1089574 1089883 310 - 1.486 1.181 -1.065
ENSG00000099817 E022 1520.293192 9.032081e-05 4.576372e-06 3.287127e-05 19 1089884 1089896 13 - 2.996 3.057 0.202
ENSG00000099817 E023 2824.696810 2.813687e-04 5.321807e-02 1.148828e-01 19 1089897 1089962 66 - 3.296 3.321 0.083
ENSG00000099817 E024 2794.472594 1.522450e-04 5.805726e-01 7.052874e-01 19 1090087 1090141 55 - 3.315 3.312 -0.012
ENSG00000099817 E025 1814.903734 1.036066e-04 1.446363e-02 3.927657e-02 19 1090142 1090145 4 - 3.147 3.120 -0.089
ENSG00000099817 E026 3224.346477 4.325702e-05 2.194320e-05 1.347555e-04 19 1090908 1090988 81 - 3.401 3.368 -0.112
ENSG00000099817 E027 7.149336 2.616772e-03 1.261105e-04 6.418067e-04 19 1091501 1091791 291 - 1.140 0.664 -1.823
ENSG00000099817 E028 3363.307668 2.626852e-04 8.733991e-03 2.562127e-02 19 1091792 1091907 116 - 3.413 3.388 -0.083
ENSG00000099817 E029 7.999469 2.085446e-03 9.946861e-05 5.193911e-04 19 1092313 1092346 34 - 1.180 0.720 -1.737
ENSG00000099817 E030 5.726129 6.985710e-02 2.787365e-03 9.636796e-03 19 1093730 1093755 26 - 1.072 0.574 -1.975
ENSG00000099817 E031 14.599666 1.226518e-03 1.455846e-08 1.741307e-07 19 1093756 1093903 148 - 1.446 0.924 -1.867
ENSG00000099817 E032 3630.433532 1.775164e-03 2.714240e-02 6.628910e-02 19 1093904 1094078 175 - 3.461 3.420 -0.137
ENSG00000099817 E033 19.349363 1.002695e-03 7.247659e-05 3.921200e-04 19 1094079 1095258 1180 - 1.446 1.107 -1.194
ENSG00000099817 E034 2006.018097 3.902435e-03 1.123327e-01 2.089578e-01 19 1095259 1095384 126 - 3.206 3.162 -0.149