ENSG00000099804

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215574 ENSG00000099804 HEK293_OSMI2_2hA HEK293_TMG_2hB CDC34 protein_coding protein_coding 176.3326 332.5581 95.48151 14.01252 1.518842 -1.800206 124.605316 225.91509 71.694281 20.951364 0.7225647 -1.655713 0.71977083 0.67680000 0.75123333 0.07443333 3.437965e-01 2.714517e-151 FALSE TRUE
ENST00000586283 ENSG00000099804 HEK293_OSMI2_2hA HEK293_TMG_2hB CDC34 protein_coding protein_coding 176.3326 332.5581 95.48151 14.01252 1.518842 -1.800206 11.135318 19.33824 4.121621 1.258258 0.7977140 -2.227422 0.06077083 0.05843333 0.04320000 -0.01523333 4.793351e-01 2.714517e-151 FALSE FALSE
ENST00000606400 ENSG00000099804 HEK293_OSMI2_2hA HEK293_TMG_2hB CDC34 protein_coding retained_intron 176.3326 332.5581 95.48151 14.01252 1.518842 -1.800206 10.642412 24.29317 1.068638 1.952868 0.8657029 -4.493862 0.04546250 0.07326667 0.01103333 -0.06223333 1.204249e-01 2.714517e-151 FALSE TRUE
MSTRG.15990.2 ENSG00000099804 HEK293_OSMI2_2hA HEK293_TMG_2hB CDC34 protein_coding   176.3326 332.5581 95.48151 14.01252 1.518842 -1.800206 5.740661 0.00000 12.136632 0.000000 0.4425085 10.246341 0.03922083 0.00000000 0.12703333 0.12703333 2.714517e-151 2.714517e-151 FALSE TRUE
MSTRG.15990.5 ENSG00000099804 HEK293_OSMI2_2hA HEK293_TMG_2hB CDC34 protein_coding   176.3326 332.5581 95.48151 14.01252 1.518842 -1.800206 9.781515 27.03220 0.000000 1.083036 0.0000000 -11.400997 0.05712500 0.08136667 0.00000000 -0.08136667 1.144875e-84 2.714517e-151 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099804 E001 1.665031 8.325744e-03 9.232887e-07 7.718419e-06 19 531665 531704 40 + 0.865 0.068 -5.233
ENSG00000099804 E002 6.233589 3.459692e-03 1.431899e-16 6.045663e-15 19 531705 531720 16 + 1.352 0.337 -4.197
ENSG00000099804 E003 37.896202 1.669346e-02 2.238753e-23 2.060860e-21 19 531721 531759 39 + 2.006 1.152 -2.927
ENSG00000099804 E004 40.136499 1.562300e-02 6.607684e-26 7.833579e-24 19 531760 531768 9 + 2.040 1.162 -3.006
ENSG00000099804 E005 65.849666 9.900540e-03 3.971655e-48 2.174619e-45 19 531769 531818 50 + 2.282 1.311 -3.288
ENSG00000099804 E006 519.089339 3.418484e-03 5.129181e-16 2.013485e-14 19 531819 531994 176 + 2.809 2.536 -0.907
ENSG00000099804 E007 637.506861 3.570045e-04 7.129428e-13 1.791787e-11 19 531995 532031 37 + 2.799 2.658 -0.469
ENSG00000099804 E008 1161.078547 9.303215e-05 3.693929e-09 4.926807e-08 19 532032 532099 68 + 3.015 2.931 -0.281
ENSG00000099804 E009 875.969149 9.551765e-05 1.328877e-01 2.381262e-01 19 532100 532108 9 + 2.852 2.821 -0.103
ENSG00000099804 E010 1615.627296 6.092291e-05 4.826968e-02 1.061148e-01 19 535837 535923 87 + 3.116 3.086 -0.099
ENSG00000099804 E011 1248.718316 8.477123e-05 8.950616e-03 2.615681e-02 19 536243 536278 36 + 3.014 2.972 -0.137
ENSG00000099804 E012 1548.827995 4.514261e-04 2.223873e-01 3.532462e-01 19 536279 536340 62 + 3.066 3.077 0.036
ENSG00000099804 E013 22.929967 8.582038e-04 5.972757e-04 2.523688e-03 19 536341 536715 375 + 1.478 1.197 -0.980
ENSG00000099804 E014 9.445917 2.889939e-02 3.507984e-02 8.180115e-02 19 536953 537012 60 + 1.135 0.840 -1.094
ENSG00000099804 E015 2535.528640 7.015772e-05 9.118339e-03 2.657930e-02 19 537013 537147 135 + 3.274 3.290 0.052
ENSG00000099804 E016 199.470798 4.883514e-04 1.636650e-04 8.078437e-04 19 538678 539024 347 + 2.286 2.160 -0.422
ENSG00000099804 E017 28.467609 1.285509e-02 9.906135e-01 9.983018e-01 19 541217 541252 36 + 1.364 1.363 -0.004
ENSG00000099804 E018 1072.114040 1.143957e-04 1.885100e-06 1.475059e-05 19 541339 541342 4 + 2.863 2.924 0.203
ENSG00000099804 E019 1105.788397 1.263384e-04 2.047808e-06 1.589380e-05 19 541343 541345 3 + 2.877 2.938 0.201
ENSG00000099804 E020 1319.421573 9.134899e-05 8.731622e-08 8.965450e-07 19 541346 541360 15 + 2.953 3.014 0.204
ENSG00000099804 E021 4585.198051 7.577545e-05 1.065376e-38 3.403579e-36 19 541361 542092 732 + 3.477 3.559 0.271