ENSG00000099308

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262811 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding protein_coding 3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.4316108 1.08005448 0.19762158 0.55277321 0.19762158 -2.3923719 0.13622917 0.238900000 0.07780000 -0.16110000 5.705572e-01 8.636828e-06 FALSE TRUE
MSTRG.16715.1 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding   3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.1842374 0.01398401 0.37404634 0.01398401 0.09637200 4.0011354 0.07457083 0.004133333 0.14443333 0.14030000 8.636828e-06 8.636828e-06 FALSE TRUE
MSTRG.16715.10 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding   3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.4422199 0.63313889 0.36276769 0.45697189 0.22878875 -0.7868535 0.13818333 0.177800000 0.13793333 -0.03986667 1.000000e+00 8.636828e-06 FALSE TRUE
MSTRG.16715.2 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding   3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.4941866 0.00000000 0.78153213 0.00000000 0.47309727 6.3065760 0.16203750 0.000000000 0.32163333 0.32163333 1.782609e-01 8.636828e-06 FALSE TRUE
MSTRG.16715.4 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding   3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.5881130 1.09967592 0.38842222 0.23106897 0.38842222 -1.4777684 0.16057083 0.278166667 0.14183333 -0.13633333 2.965967e-01 8.636828e-06 FALSE TRUE
MSTRG.16715.7 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding   3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.2243631 0.00000000 0.00000000 0.00000000 0.00000000 0.0000000 0.06444167 0.000000000 0.00000000 0.00000000   8.636828e-06 FALSE TRUE
MSTRG.16715.8 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding   3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.2452938 0.29369304 0.00000000 0.29369304 0.00000000 -4.9245419 0.08747917 0.065433333 0.00000000 -0.06543333 8.536034e-01 8.636828e-06 FALSE TRUE
MSTRG.16715.9 ENSG00000099308 HEK293_OSMI2_2hA HEK293_TMG_2hB MAST3 protein_coding   3.186142 4.140691 2.554356 0.3774004 0.1023843 -0.6947552 0.3721899 0.50368815 0.04994088 0.30700392 0.04994088 -3.0992807 0.11059583 0.111200000 0.02093333 -0.09026667 6.025181e-01 8.636828e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099308 E001 0.5901540 0.0232940606 1.259285e-01 2.284738e-01 19 18097772 18097777 6 + 0.343 0.102 -2.191
ENSG00000099308 E002 0.8825757 0.0154132573 2.067820e-02 5.290811e-02 19 18097778 18097787 10 + 0.478 0.102 -2.927
ENSG00000099308 E003 1.1781179 0.0182136329 3.145532e-02 7.479562e-02 19 18097788 18097792 5 + 0.532 0.184 -2.192
ENSG00000099308 E004 2.5410712 0.0070251023 5.733724e-01 6.993001e-01 19 18097793 18097831 39 + 0.581 0.528 -0.243
ENSG00000099308 E005 2.9913511 0.0055600114 1.666022e-01 2.837732e-01 19 18107587 18107618 32 + 0.700 0.528 -0.758
ENSG00000099308 E006 1.2020764 0.4009403432 8.858962e-01 9.310396e-01 19 18110170 18110331 162 + 0.342 0.319 -0.141
ENSG00000099308 E007 0.4720498 0.2238117748 3.607262e-01 5.050775e-01 19 18110332 18110366 35 + 0.255 0.102 -1.597
ENSG00000099308 E008 1.1018559 0.0170593752 7.449947e-01 8.331533e-01 19 18110652 18110694 43 + 0.343 0.313 -0.191
ENSG00000099308 E009 1.2501491 0.0418248432 9.645951e-01 9.816796e-01 19 18110695 18110741 47 + 0.343 0.364 0.124
ENSG00000099308 E010 1.0610564 0.2396476298 8.982874e-01 9.391144e-01 19 18118062 18118106 45 + 0.256 0.317 0.425
ENSG00000099308 E011 1.0610564 0.2396476298 8.982874e-01 9.391144e-01 19 18118107 18118107 1 + 0.256 0.317 0.425
ENSG00000099308 E012 4.4652081 0.0037889208 5.683528e-01 6.950485e-01 19 18118108 18118254 147 + 0.734 0.696 -0.155
ENSG00000099308 E013 10.8727266 0.0016517583 5.963826e-01 7.183460e-01 19 18121685 18121773 89 + 1.058 1.053 -0.019
ENSG00000099308 E014 3.4224970 0.0045539514 2.659817e-01 4.041037e-01 19 18121850 18121852 3 + 0.478 0.695 0.981
ENSG00000099308 E015 10.7567848 0.0015829688 9.638210e-01 9.811606e-01 19 18121853 18121922 70 + 1.010 1.063 0.195
ENSG00000099308 E016 5.4669308 0.0031145362 4.255003e-01 5.684429e-01 19 18122107 18122127 21 + 0.821 0.760 -0.240
ENSG00000099308 E017 16.3624609 0.0010937344 2.204531e-01 3.509535e-01 19 18122673 18122751 79 + 1.252 1.198 -0.189
ENSG00000099308 E018 22.0710843 0.0014176926 8.312302e-02 1.644494e-01 19 18123217 18123374 158 + 1.385 1.307 -0.271
ENSG00000099308 E019 0.0000000       19 18123399 18123579 181 +      
ENSG00000099308 E020 16.7202672 0.0331928528 2.829097e-01 4.229713e-01 19 18123580 18123655 76 + 1.261 1.200 -0.217
ENSG00000099308 E021 23.6090892 0.0449212321 6.834766e-02 1.406426e-01 19 18123939 18124148 210 + 1.468 1.303 -0.571
ENSG00000099308 E022 17.9975603 0.0308990992 2.020287e-01 3.287358e-01 19 18124265 18124366 102 + 1.306 1.221 -0.299
ENSG00000099308 E023 20.6383734 0.0041973462 9.656639e-02 1.852128e-01 19 18124642 18124774 133 + 1.355 1.273 -0.286
ENSG00000099308 E024 14.9257940 0.0016663257 5.091723e-01 6.445138e-01 19 18128400 18128458 59 + 1.102 1.213 0.397
ENSG00000099308 E025 17.0574575 0.0011753583 3.576979e-01 5.021150e-01 19 18128866 18128951 86 + 1.141 1.272 0.463
ENSG00000099308 E026 0.8178402 0.1385289408 4.690510e-01 6.083631e-01 19 18128952 18129359 408 + 0.147 0.310 1.377
ENSG00000099308 E027 26.4372389 0.0010218599 4.121045e-01 5.556644e-01 19 18130494 18130702 209 + 1.413 1.408 -0.018
ENSG00000099308 E028 27.0916655 0.0027320123 4.241208e-02 9.546761e-02 19 18131909 18132047 139 + 1.476 1.386 -0.310
ENSG00000099308 E029 31.5621841 0.0062734464 7.544585e-02 1.522329e-01 19 18134579 18134711 133 + 1.530 1.457 -0.251
ENSG00000099308 E030 34.0282149 0.0063424207 6.174163e-02 1.295581e-01 19 18134817 18134982 166 + 1.565 1.488 -0.262
ENSG00000099308 E031 28.7486582 0.0063376826 5.991465e-03 1.858812e-02 19 18135740 18135841 102 + 1.541 1.390 -0.516
ENSG00000099308 E032 32.7284609 0.0007334526 1.161898e-02 3.262580e-02 19 18137239 18137354 116 + 1.565 1.462 -0.351
ENSG00000099308 E033 14.0146125 0.0013068027 1.878865e-02 4.887144e-02 19 18137355 18137361 7 + 1.252 1.092 -0.570
ENSG00000099308 E034 28.3799916 0.0016989501 2.040020e-01 3.311817e-01 19 18139015 18139124 110 + 1.464 1.430 -0.117
ENSG00000099308 E035 29.0230402 0.0007765735 5.065466e-01 6.422028e-01 19 18141882 18142015 134 + 1.385 1.482 0.333
ENSG00000099308 E036 27.2373575 0.0008823025 9.366983e-01 9.641899e-01 19 18143763 18143947 185 + 1.385 1.442 0.197
ENSG00000099308 E037 16.6665980 0.0011050922 7.993593e-02 1.594636e-01 19 18143948 18144007 60 + 1.280 1.184 -0.340
ENSG00000099308 E038 19.9300145 0.0211979143 2.764073e-01 4.158655e-01 19 18144466 18144549 84 + 1.323 1.274 -0.173
ENSG00000099308 E039 23.9949148 0.0008269116 7.110975e-01 8.081028e-01 19 18144550 18144693 144 + 1.355 1.380 0.085
ENSG00000099308 E040 26.3621731 0.0007753154 9.196097e-01 9.532113e-01 19 18145003 18145141 139 + 1.385 1.429 0.153
ENSG00000099308 E041 23.6160935 0.0007896768 3.400777e-01 4.840314e-01 19 18145142 18145229 88 + 1.378 1.360 -0.061
ENSG00000099308 E042 20.9720700 0.0015730736 2.637183e-01 4.016282e-01 19 18145743 18145865 123 + 1.332 1.296 -0.124
ENSG00000099308 E043 17.4884224 0.0020863516 6.220875e-01 7.389321e-01 19 18146881 18147017 137 + 1.232 1.240 0.031
ENSG00000099308 E044 4.1652106 0.0040303321 6.206044e-01 7.377310e-01 19 18147018 18147044 27 + 0.624 0.739 0.483
ENSG00000099308 E045 13.0214230 0.0072212193 5.518743e-02 1.183131e-01 19 18147443 18147624 182 + 0.936 1.192 0.934
ENSG00000099308 E046 295.7811152 0.0127986788 5.766340e-07 5.042307e-06 19 18149191 18151791 2601 + 2.250 2.532 0.940