ENSG00000099250

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374822 ENSG00000099250 HEK293_OSMI2_2hA HEK293_TMG_2hB NRP1 protein_coding protein_coding 4.57723 3.451428 6.118316 0.2250533 0.1414951 0.8241234 0.7755875 0.1940428 1.9454026 0.1552089 0.2001803 3.26052195 0.1486208 0.05150000 0.3169667 0.2654667 0.02456825 0.02456825 FALSE TRUE
ENST00000374867 ENSG00000099250 HEK293_OSMI2_2hA HEK293_TMG_2hB NRP1 protein_coding protein_coding 4.57723 3.451428 6.118316 0.2250533 0.1414951 0.8241234 1.9981394 1.9928008 2.0826371 0.4106927 0.2227920 0.06330321 0.4824250 0.59320000 0.3407333 -0.2524667 0.39982826 0.02456825 FALSE TRUE
ENST00000374875 ENSG00000099250 HEK293_OSMI2_2hA HEK293_TMG_2hB NRP1 protein_coding protein_coding 4.57723 3.451428 6.118316 0.2250533 0.1414951 0.8241234 0.1242807 0.2350900 0.0000000 0.2350900 0.0000000 -4.61523993 0.0401750 0.06040000 0.0000000 -0.0604000 0.65132312 0.02456825 FALSE TRUE
MSTRG.3774.5 ENSG00000099250 HEK293_OSMI2_2hA HEK293_TMG_2hB NRP1 protein_coding   4.57723 3.451428 6.118316 0.2250533 0.1414951 0.8241234 0.5687311 0.1927737 0.9826365 0.1927737 0.3191571 2.29139472 0.0956000 0.05826667 0.1619667 0.1037000 0.27542906 0.02456825 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000099250 E001 0.1817044 0.0402503478 3.508535e-01   10 33177492 33177492 1 - 0.000 0.144 10.053
ENSG00000099250 E002 0.1817044 0.0402503478 3.508535e-01   10 33177493 33177493 1 - 0.000 0.144 12.425
ENSG00000099250 E003 71.9843950 0.0223850141 2.440260e-05 1.484204e-04 10 33177494 33178429 936 - 1.664 2.017 1.192
ENSG00000099250 E004 134.2928885 0.0003349202 3.232110e-10 5.232554e-09 10 33178430 33179896 1467 - 2.030 2.223 0.647
ENSG00000099250 E005 36.4978330 0.0063986927 2.019906e-01 3.286909e-01 10 33179897 33180098 202 - 1.521 1.618 0.329
ENSG00000099250 E006 33.0932469 0.0007057503 3.926244e-01 5.365661e-01 10 33180099 33180365 267 - 1.545 1.504 -0.140
ENSG00000099250 E007 14.4321608 0.0015964678 2.427009e-01 3.773438e-01 10 33182698 33182748 51 - 1.129 1.242 0.402
ENSG00000099250 E008 20.1948319 0.0066766552 5.408824e-01 6.719508e-01 10 33185628 33185724 97 - 1.339 1.298 -0.145
ENSG00000099250 E009 31.3525702 0.0006989020 4.549255e-02 1.010971e-01 10 33186217 33186488 272 - 1.555 1.441 -0.391
ENSG00000099250 E010 17.7158956 0.0192401916 4.342247e-02 9.733602e-02 10 33192281 33192418 138 - 1.350 1.145 -0.724
ENSG00000099250 E011 0.0000000       10 33194367 33194371 5 -      
ENSG00000099250 E012 2.4032869 0.4228649369 7.755459e-01 8.553978e-01 10 33194372 33194724 353 - 0.461 0.627 0.772
ENSG00000099250 E013 0.8836199 0.1663282452 7.090090e-01 8.065046e-01 10 33194725 33194780 56 - 0.318 0.248 -0.490
ENSG00000099250 E014 1.0404339 0.0121218753 1.217656e-01 2.224409e-01 10 33195424 33195576 153 - 0.419 0.143 -2.053
ENSG00000099250 E015 1.1091160 0.2714135615 2.501815e-01 3.861507e-01 10 33197621 33197649 29 - 0.188 0.462 1.813
ENSG00000099250 E016 19.2681490 0.0010207071 8.241535e-01 8.892133e-01 10 33197650 33197709 60 - 1.311 1.305 -0.022
ENSG00000099250 E017 0.8481309 0.0143524348 4.105158e-01 5.541099e-01 10 33201018 33202890 1873 - 0.188 0.337 1.119
ENSG00000099250 E018 11.9255347 0.0016435317 7.622948e-01 8.458993e-01 10 33202891 33202911 21 - 1.092 1.131 0.141
ENSG00000099250 E019 28.5419832 0.0007175781 5.777481e-01 7.029578e-01 10 33202912 33202995 84 - 1.452 1.499 0.160
ENSG00000099250 E020 0.0000000       10 33205449 33206353 905 -      
ENSG00000099250 E021 49.7662594 0.0006611727 4.598009e-01 5.999002e-01 10 33207572 33207716 145 - 1.687 1.734 0.160
ENSG00000099250 E022 6.0776877 0.0028392376 8.027722e-01 8.743958e-01 10 33211410 33213385 1976 - 0.859 0.837 -0.084
ENSG00000099250 E023 25.9411741 0.0008021556 7.253348e-01 8.186430e-01 10 33213386 33213391 6 - 1.435 1.422 -0.044
ENSG00000099250 E024 75.4425049 0.0046989501 7.507246e-02 1.516555e-01 10 33213392 33213717 326 - 1.916 1.837 -0.265
ENSG00000099250 E025 36.9694627 0.0005740530 2.920309e-03 1.003089e-02 10 33221719 33221863 145 - 1.644 1.482 -0.551
ENSG00000099250 E026 45.3435911 0.0372590266 2.300731e-01 3.625544e-01 10 33226134 33226289 156 - 1.711 1.606 -0.356
ENSG00000099250 E027 0.5159433 0.2470206016 8.466952e-01 9.047184e-01 10 33249414 33249549 136 - 0.188 0.146 -0.442
ENSG00000099250 E028 41.5046901 0.0011036319 1.099549e-01 2.054810e-01 10 33254028 33254194 167 - 1.659 1.582 -0.263
ENSG00000099250 E029 42.2760356 0.0005259884 1.453063e-02 3.943477e-02 10 33256316 33256471 156 - 1.684 1.564 -0.410
ENSG00000099250 E030 48.5066503 0.0017719567 2.884910e-02 6.970076e-02 10 33263646 33263873 228 - 1.737 1.632 -0.355
ENSG00000099250 E031 39.0045080 0.0042153491 6.047868e-03 1.873703e-02 10 33270675 33270856 182 - 1.664 1.500 -0.561
ENSG00000099250 E032 29.9080969 0.0018661056 4.966628e-03 1.583682e-02 10 33330708 33330882 175 - 1.558 1.382 -0.606
ENSG00000099250 E033 24.5813637 0.0007816965 9.325267e-01 9.614911e-01 10 33334310 33334840 531 - 1.399 1.416 0.057
ENSG00000099250 E034 0.2998086 0.0291444279 8.046501e-01   10 33334841 33334905 65 - 0.104 0.143 0.530
ENSG00000099250 E035 0.7385489 0.0721386423 5.005800e-02 1.092702e-01 10 33334990 33335078 89 - 0.372 0.000 -14.664
ENSG00000099250 E036 0.1451727 0.0432007556 6.676560e-01   10 33336195 33336262 68 - 0.104 0.000 -12.615