ENSG00000097096

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000234668 ENSG00000097096 HEK293_OSMI2_2hA HEK293_TMG_2hB SYDE2 protein_coding retained_intron 1.49114 0.2168071 2.451446 0.01004944 0.134884 3.439968 0.3929077 0.03361815 0.7433171 0.01054080 0.008153233 4.110257 0.1978542 0.1563667 0.3053667 0.1490000 0.56994587 0.01256855 FALSE TRUE
ENST00000341460 ENSG00000097096 HEK293_OSMI2_2hA HEK293_TMG_2hB SYDE2 protein_coding protein_coding 1.49114 0.2168071 2.451446 0.01004944 0.134884 3.439968 0.7682764 0.18318895 0.9560916 0.01589426 0.078145425 2.322147 0.6735708 0.8436333 0.3938333 -0.4498000 0.01256855 0.01256855 FALSE TRUE
MSTRG.1515.2 ENSG00000097096 HEK293_OSMI2_2hA HEK293_TMG_2hB SYDE2 protein_coding   1.49114 0.2168071 2.451446 0.01004944 0.134884 3.439968 0.2886656 0.00000000 0.7305281 0.00000000 0.200271258 6.210482 0.1086917 0.0000000 0.2909333 0.2909333 0.03503080 0.01256855 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000097096 E001 23.045602 0.0014991307 1.487639e-07 1.461312e-06 1 85156889 85158766 1878 - 1.123 1.558 1.519
ENSG00000097096 E002 10.350067 0.0258223931 9.412353e-02 1.815827e-01 1 85158767 85158969 203 - 0.853 1.095 0.901
ENSG00000097096 E003 12.750176 0.0204571239 7.090014e-01 8.065027e-01 1 85158970 85159249 280 - 0.981 0.934 -0.177
ENSG00000097096 E004 10.262546 0.0276687854 2.091657e-01 3.375282e-01 1 85164526 85164757 232 - 0.866 1.056 0.709
ENSG00000097096 E005 8.864394 0.0098752694 7.825551e-01 8.603919e-01 1 85169044 85169225 182 - 0.833 0.883 0.193
ENSG00000097096 E006 9.897304 0.0065648661 7.082779e-02 1.447243e-01 1 85178146 85178272 127 - 0.905 0.585 -1.302
ENSG00000097096 E007 8.376034 0.0021665960 1.516798e-01 2.639486e-01 1 85181159 85182097 939 - 0.839 0.585 -1.052
ENSG00000097096 E008 53.143289 0.0006951179 2.774197e-02 6.750193e-02 1 85182098 85183200 1103 - 1.572 1.426 -0.504
ENSG00000097096 E009 16.090807 0.0200547672 3.127611e-01 4.552882e-01 1 85190057 85190209 153 - 1.047 1.182 0.485
ENSG00000097096 E010 15.374332 0.0022170245 6.999775e-01 7.995697e-01 1 85190210 85190474 265 - 1.038 1.093 0.200
ENSG00000097096 E011 16.484087 0.0010945596 1.653250e-02 4.391268e-02 1 85190475 85190752 278 - 1.104 0.760 -1.302
ENSG00000097096 E012 21.850560 0.0014573015 2.630371e-03 9.160891e-03 1 85200252 85201724 1473 - 1.220 0.826 -1.454