ENSG00000096717

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000212015 ENSG00000096717 HEK293_OSMI2_2hA HEK293_TMG_2hB SIRT1 protein_coding protein_coding 12.69475 4.793198 22.37475 0.5139666 0.6335807 2.220449 9.5727742 2.6923496 19.246684 0.09056423 0.9616381 2.8330731 0.6899000 0.5791333 0.85923333 0.28010000 4.488210e-03 6.922485e-08 FALSE TRUE
ENST00000406900 ENSG00000096717 HEK293_OSMI2_2hA HEK293_TMG_2hB SIRT1 protein_coding protein_coding 12.69475 4.793198 22.37475 0.5139666 0.6335807 2.220449 0.8514541 1.7856555 1.038291 0.50963514 0.1376330 -0.7764711 0.1099417 0.3609333 0.04640000 -0.31453333 6.922485e-08 6.922485e-08 FALSE TRUE
MSTRG.4063.2 ENSG00000096717 HEK293_OSMI2_2hA HEK293_TMG_2hB SIRT1 protein_coding   12.69475 4.793198 22.37475 0.5139666 0.6335807 2.220449 1.9293347 0.2732244 1.870159 0.27322442 0.3708741 2.7308369 0.1427542 0.0521000 0.08436667 0.03226667 3.808689e-01 6.922485e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000096717 E001 88.246497 0.0003067486 1.276581e-06 1.036535e-05 10 67884656 67885151 496 + 1.845 1.698 -0.494
ENSG00000096717 E002 0.000000       10 67885181 67885183 3 +      
ENSG00000096717 E003 1.918731 0.0078112071 4.999827e-02 1.091737e-01 10 67885184 67885399 216 + 0.434 0.000 -11.163
ENSG00000096717 E004 76.508523 0.0003824108 2.812045e-04 1.303307e-03 10 67887417 67887533 117 + 1.775 1.684 -0.309
ENSG00000096717 E005 123.528186 0.0002425087 7.922248e-04 3.231547e-03 10 67888882 67889123 242 + 1.972 1.943 -0.100
ENSG00000096717 E006 84.972284 0.0003232773 2.080110e-02 5.317232e-02 10 67891402 67891483 82 + 1.809 1.803 -0.019
ENSG00000096717 E007 100.861580 0.0002960151 1.878355e-02 4.886087e-02 10 67891484 67891554 71 + 1.880 1.883 0.010
ENSG00000096717 E008 0.147249 0.0445079756 1.000000e+00   10 67906050 67906162 113 + 0.054 0.000 -7.462
ENSG00000096717 E009 2.951676 0.0069675673 3.431088e-01 4.871684e-01 10 67906163 67906287 125 + 0.528 0.415 -0.568
ENSG00000096717 E010 129.716515 0.0002325832 1.006723e-05 6.684451e-05 10 67906790 67906937 148 + 2.000 1.926 -0.247
ENSG00000096717 E011 86.852090 0.0003893409 5.742556e-03 1.793485e-02 10 67908046 67908125 80 + 1.821 1.792 -0.097
ENSG00000096717 E012 129.905166 0.0009180133 1.305304e-03 4.994665e-03 10 67909256 67909442 187 + 1.993 1.966 -0.091
ENSG00000096717 E013 309.912057 0.0002276860 9.441948e-08 9.628835e-07 10 67912474 67913031 558 + 2.368 2.342 -0.085
ENSG00000096717 E014 680.586405 0.0078583578 4.213413e-12 9.326071e-11 10 67916265 67918390 2126 + 2.636 2.947 1.032