ENSG00000091542

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000399138 ENSG00000091542 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH5 protein_coding protein_coding 130.507 223.8984 78.22036 8.485229 3.185551 -1.517108 65.27461 92.04512 47.868550 10.802957 0.5581873 -0.9431186 0.5248167 0.4086667 0.61436667 0.2057000 0.003247430 0.00324743 FALSE TRUE
ENST00000541285 ENSG00000091542 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH5 protein_coding protein_coding 130.507 223.8984 78.22036 8.485229 3.185551 -1.517108 16.80131 23.71948 12.639371 6.723857 2.0533926 -0.9076145 0.1395625 0.1039667 0.16056667 0.0566000 0.467694420 0.00324743 FALSE FALSE
MSTRG.13788.3 ENSG00000091542 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH5 protein_coding   130.507 223.8984 78.22036 8.485229 3.185551 -1.517108 15.97361 18.93309 12.738571 2.004945 1.9259680 -0.5713365 0.1336125 0.0841000 0.16170000 0.0776000 0.006401206 0.00324743   FALSE
MSTRG.13788.5 ENSG00000091542 HEK293_OSMI2_2hA HEK293_TMG_2hB ALKBH5 protein_coding   130.507 223.8984 78.22036 8.485229 3.185551 -1.517108 27.69447 82.64577 1.845206 12.605553 0.9671040 -5.4774646 0.1618667 0.3746667 0.02283333 -0.3518333 0.055022697 0.00324743   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000091542 E001 1.845574 0.0120017088 1.410919e-01 2.495373e-01 17 18183078 18183085 8 + 0.546 0.355 -0.995
ENSG00000091542 E002 8.541535 0.0022902228 1.870156e-02 4.867900e-02 17 18183086 18183127 42 + 1.044 0.864 -0.672
ENSG00000091542 E003 28.982004 0.0107534272 7.037872e-04 2.912606e-03 17 18183128 18183197 70 + 1.540 1.360 -0.623
ENSG00000091542 E004 31.558649 0.0124283150 1.095823e-03 4.289971e-03 17 18183198 18183224 27 + 1.571 1.399 -0.590
ENSG00000091542 E005 35.049187 0.0007882158 2.814752e-03 9.717297e-03 17 18183225 18183265 41 + 1.559 1.469 -0.310
ENSG00000091542 E006 165.956853 0.0032186562 1.658890e-10 2.820604e-09 17 18183314 18183644 331 + 2.266 2.118 -0.495
ENSG00000091542 E007 2083.562448 0.0057319698 1.219729e-06 9.942481e-06 17 18183828 18185013 1186 + 3.289 3.249 -0.133
ENSG00000091542 E008 4.832568 0.0035181572 4.388596e-01 5.806848e-01 17 18185711 18185879 169 + 0.743 0.716 -0.107
ENSG00000091542 E009 697.705658 0.0005754994 3.523322e-13 9.269308e-12 17 18194955 18195035 81 + 2.802 2.778 -0.082
ENSG00000091542 E010 5.695281 0.0070142026 6.658072e-01 7.734321e-01 17 18206561 18206814 254 + 0.743 0.767 0.100
ENSG00000091542 E011 1036.360973 0.0010970250 6.880730e-09 8.742094e-08 17 18206815 18206970 156 + 2.957 2.956 -0.005
ENSG00000091542 E012 7602.567095 0.0041540949 1.077467e-12 2.627741e-11 17 18208219 18209954 1736 + 3.665 3.866 0.667