ENSG00000089876

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000284690 ENSG00000089876 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX32 protein_coding protein_coding 9.256535 5.40913 12.18147 0.532518 0.2762044 1.169739 5.0962762 3.731513 4.7835388 0.7266835 0.3080061 0.3574695 0.58442083 0.6786 0.3939333 -0.2846667 1.254575e-02 3.28706e-09 FALSE TRUE
ENST00000415732 ENSG00000089876 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX32 protein_coding protein_coding 9.256535 5.40913 12.18147 0.532518 0.2762044 1.169739 0.3184001 0.000000 0.8466362 0.0000000 0.0788998 6.4206107 0.02748333 0.0000 0.0693000 0.0693000 3.287060e-09 3.28706e-09 FALSE FALSE
MSTRG.4799.1 ENSG00000089876 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX32 protein_coding   9.256535 5.40913 12.18147 0.532518 0.2762044 1.169739 3.2186232 1.372672 6.1104252 0.2334988 0.4799146 2.1461728 0.31649167 0.2659 0.5004333 0.2345333 5.422552e-02 3.28706e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000089876 E001 90.1278729 0.0124693101 1.901436e-06 1.486552e-05 10 125836337 125836734 398 - 1.800 2.117 1.067
ENSG00000089876 E002 47.0369722 0.0078916051 4.659504e-04 2.029915e-03 10 125836735 125836743 9 - 1.566 1.814 0.843
ENSG00000089876 E003 91.3088808 0.0141546346 1.069128e-03 4.198134e-03 10 125836744 125836855 112 - 1.850 2.089 0.802
ENSG00000089876 E004 104.7811017 0.0024299353 1.580936e-04 7.832618e-04 10 125838206 125838387 182 - 1.946 2.114 0.565
ENSG00000089876 E005 0.8481309 0.0145345562 2.698854e-01 4.084393e-01 10 125838388 125839000 613 - 0.169 0.369 1.499
ENSG00000089876 E006 104.6433313 0.0002940591 2.748315e-02 6.697454e-02 10 125839001 125839188 188 - 1.978 2.068 0.302
ENSG00000089876 E007 1.7003141 0.0110439591 6.605758e-02 1.368084e-01 10 125839763 125840846 1084 - 0.290 0.615 1.716
ENSG00000089876 E008 106.6340381 0.0004900907 2.299690e-01 3.624266e-01 10 125840847 125840996 150 - 2.001 2.057 0.188
ENSG00000089876 E009 0.1515154 0.0431775578 9.111552e-01   10 125840997 125841742 746 - 0.092 0.001 -7.626
ENSG00000089876 E010 103.6073570 0.0003387242 9.841620e-01 9.941960e-01 10 125841743 125841934 192 - 2.006 2.021 0.052
ENSG00000089876 E011 1.4005055 0.0125229030 6.444135e-02 1.341106e-01 10 125841935 125842957 1023 - 0.233 0.565 1.909
ENSG00000089876 E012 4.7824000 0.0071966798 7.109022e-01 8.079761e-01 10 125851339 125852292 954 - 0.777 0.737 -0.159
ENSG00000089876 E013 76.4082108 0.0089401523 9.276638e-01 9.584961e-01 10 125852293 125852451 159 - 1.879 1.882 0.008
ENSG00000089876 E014 65.8401405 0.0004014635 1.791771e-02 4.695804e-02 10 125852543 125852642 100 - 1.850 1.758 -0.309
ENSG00000089876 E015 110.1613894 0.0002909789 1.417534e-03 5.365156e-03 10 125853961 125854203 243 - 2.073 1.979 -0.318
ENSG00000089876 E016 1.1187827 0.0452144809 7.096268e-01 8.069477e-01 10 125854204 125854859 656 - 0.340 0.277 -0.409
ENSG00000089876 E017 130.4627646 0.0003610512 3.573667e-04 1.608736e-03 10 125859603 125859975 373 - 2.148 2.050 -0.330
ENSG00000089876 E018 58.9728311 0.0003983887 3.615011e-01 5.058895e-01 10 125866990 125867128 139 - 1.782 1.754 -0.093
ENSG00000089876 E019 37.3197590 0.0050916720 3.921954e-01 5.361387e-01 10 125867129 125867183 55 - 1.593 1.548 -0.154
ENSG00000089876 E020 83.2340956 0.0064070792 1.917604e-02 4.972206e-02 10 125880543 125880874 332 - 1.958 1.838 -0.405
ENSG00000089876 E021 48.6400990 0.0123147689 6.874984e-03 2.089931e-02 10 125880875 125880983 109 - 1.752 1.555 -0.669
ENSG00000089876 E022 43.2706282 0.0175110896 1.513412e-03 5.679591e-03 10 125880984 125881071 88 - 1.720 1.449 -0.924
ENSG00000089876 E023 0.0000000       10 125881072 125881292 221 -      
ENSG00000089876 E024 40.8742982 0.0148167487 2.472917e-03 8.682505e-03 10 125885365 125885573 209 - 1.691 1.440 -0.857
ENSG00000089876 E025 16.8595302 0.0025994919 2.717192e-04 1.264714e-03 10 125885574 125885676 103 - 1.344 1.016 -1.170
ENSG00000089876 E026 23.9032462 0.0329422600 3.494409e-04 1.577893e-03 10 125896218 125896682 465 - 1.503 1.076 -1.499