ENSG00000089685

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000350051 ENSG00000089685 HEK293_OSMI2_2hA HEK293_TMG_2hB BIRC5 protein_coding protein_coding 208.6834 323.7673 145.1397 19.37941 3.064915 -1.15746 165.46193 253.41984 117.04581 15.042402 3.7915518 -1.11439 0.7962000 0.7828000 0.80613333 0.02333333 5.286984e-01 2.532373e-08 FALSE  
ENST00000374948 ENSG00000089685 HEK293_OSMI2_2hA HEK293_TMG_2hB BIRC5 protein_coding protein_coding 208.6834 323.7673 145.1397 19.37941 3.064915 -1.15746 25.34909 48.17924 13.36558 3.644369 0.4052877 -1.84911 0.1128917 0.1489333 0.09213333 -0.05680000 1.242882e-05 2.532373e-08 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000089685 E001 14.971810 0.0139032557 1.536797e-01 2.666707e-01 17 78214186 78214252 67 + 0.987 1.218 0.834
ENSG00000089685 E002 61.351808 0.0079799634 4.082565e-01 5.518546e-01 17 78214253 78214262 10 + 1.741 1.759 0.059
ENSG00000089685 E003 67.970262 0.0085850283 3.966721e-01 5.404411e-01 17 78214263 78214263 1 + 1.786 1.802 0.056
ENSG00000089685 E004 338.160338 0.0024585010 6.557174e-02 1.359999e-01 17 78214264 78214279 16 + 2.481 2.494 0.045
ENSG00000089685 E005 1185.267263 0.0017434912 2.960575e-04 1.363922e-03 17 78214280 78214316 37 + 3.041 3.031 -0.034
ENSG00000089685 E006 1673.522359 0.0008610400 5.177381e-06 3.671905e-05 17 78214317 78214364 48 + 3.185 3.184 -0.006
ENSG00000089685 E007 1544.374680 0.0003408839 1.883525e-08 2.207139e-07 17 78214365 78214384 20 + 3.148 3.151 0.009
ENSG00000089685 E008 1746.559169 0.0005580544 3.071426e-08 3.449768e-07 17 78214385 78214427 43 + 3.206 3.202 -0.013
ENSG00000089685 E009 2689.128126 0.0006088007 4.601809e-08 4.999133e-07 17 78214680 78214789 110 + 3.389 3.391 0.007
ENSG00000089685 E010 10.191509 0.0278489588 6.146389e-01 7.330418e-01 17 78214790 78214793 4 + 1.007 1.004 -0.011
ENSG00000089685 E011 22.544987 0.0023175443 1.007570e-03 3.984576e-03 17 78214794 78215099 306 + 1.455 1.279 -0.612
ENSG00000089685 E012 118.461299 0.0033333677 3.919026e-05 2.263910e-04 17 78215966 78216034 69 + 2.112 2.008 -0.348
ENSG00000089685 E013 8.489807 0.0204296485 1.448348e-03 5.468019e-03 17 78216035 78216066 32 + 1.141 0.803 -1.263
ENSG00000089685 E014 25.714511 0.0015161239 1.565526e-14 5.019194e-13 17 78216067 78216426 360 + 1.658 1.204 -1.568
ENSG00000089685 E015 27.280768 0.0008112037 1.204434e-18 6.585177e-17 17 78216427 78216663 237 + 1.707 1.204 -1.736
ENSG00000089685 E016 2321.925168 0.0003474889 1.079156e-18 5.937928e-17 17 78216664 78216781 118 + 3.345 3.319 -0.084
ENSG00000089685 E017 36.336346 0.0005805734 6.619128e-01 7.702873e-01 17 78222828 78222992 165 + 1.501 1.549 0.163
ENSG00000089685 E018 13701.378171 0.0021480632 1.659851e-11 3.334963e-10 17 78223465 78225636 2172 + 3.984 4.136 0.503