ENSG00000089351

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000424536 ENSG00000089351 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1A protein_coding protein_coding 40.83418 61.89013 26.76869 5.35625 1.260748 -1.208857 7.111398 15.649369 3.0529487 1.6168041 0.19617435 -2.3540330 0.15531667 0.25276667 0.11443333 -0.13833333 4.243307e-09 4.243307e-09 FALSE TRUE
ENST00000595596 ENSG00000089351 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1A protein_coding retained_intron 40.83418 61.89013 26.76869 5.35625 1.260748 -1.208857 2.493105 2.179856 2.4516702 0.5871447 0.21501709 0.1688016 0.06888750 0.03433333 0.09126667 0.05693333 2.359870e-04 4.243307e-09   FALSE
ENST00000600231 ENSG00000089351 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1A protein_coding nonsense_mediated_decay 40.83418 61.89013 26.76869 5.35625 1.260748 -1.208857 3.926213 6.363921 2.2473566 1.1367920 0.22397805 -1.4975467 0.09648333 0.10143333 0.08420000 -0.01723333 6.952497e-01 4.243307e-09 FALSE TRUE
ENST00000680623 ENSG00000089351 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1A protein_coding protein_coding 40.83418 61.89013 26.76869 5.35625 1.260748 -1.208857 19.797467 27.230108 13.4012233 1.1907932 0.09773327 -1.0222916 0.47887917 0.44506667 0.50306667 0.05800000 5.920751e-01 4.243307e-09 FALSE TRUE
MSTRG.16963.7 ENSG00000089351 HEK293_OSMI2_2hA HEK293_TMG_2hB GRAMD1A protein_coding   40.83418 61.89013 26.76869 5.35625 1.260748 -1.208857 2.142525 4.088509 0.9409667 0.5090445 0.30513661 -2.1076325 0.05139583 0.06583333 0.03453333 -0.03130000 1.361457e-01 4.243307e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000089351 E001 0.0000000       19 34994784 34994825 42 +      
ENSG00000089351 E002 0.0000000       19 34995994 34996291 298 +      
ENSG00000089351 E003 0.1817044 0.0394715416 1.000000e+00   19 34999387 34999436 50 + 0.000 0.079 8.337
ENSG00000089351 E004 17.7816123 0.0127105041 4.334335e-18 2.210301e-16 19 35000323 35000371 49 + 1.660 0.820 -2.995
ENSG00000089351 E005 38.3323725 0.0160614707 1.880526e-10 3.165349e-09 19 35000372 35000425 54 + 1.853 1.327 -1.796
ENSG00000089351 E006 51.0869915 0.0252347425 1.213482e-03 4.686671e-03 19 35000426 35000455 30 + 1.840 1.555 -0.967
ENSG00000089351 E007 60.9659688 0.0247534867 3.532651e-02 8.227709e-02 19 35000456 35000486 31 + 1.843 1.668 -0.591
ENSG00000089351 E008 4.4773150 0.0513513300 5.800307e-02 1.232075e-01 19 35001140 35001244 105 + 0.899 0.580 -1.304
ENSG00000089351 E009 1.9530822 0.0071393214 1.022272e-01 1.939069e-01 19 35002422 35002543 122 + 0.623 0.341 -1.421
ENSG00000089351 E010 4.4255665 0.0308799526 4.020428e-01 5.457410e-01 19 35002745 35002825 81 + 0.763 0.625 -0.576
ENSG00000089351 E011 19.7657766 0.0017484148 4.334840e-01 5.758924e-01 19 35005685 35005931 247 + 1.187 1.254 0.236
ENSG00000089351 E012 305.4769707 0.0003859484 1.388264e-02 3.796004e-02 19 35009119 35009329 211 + 2.458 2.395 -0.210
ENSG00000089351 E013 182.5108511 0.0013765692 8.573674e-01 9.119175e-01 19 35009423 35009443 21 + 2.188 2.191 0.008
ENSG00000089351 E014 0.5514428 0.0196586761 3.664770e-01 5.108078e-01 19 35009444 35009445 2 + 0.000 0.203 9.913
ENSG00000089351 E015 2.4261773 0.0284244919 1.359378e-02 3.729186e-02 19 35009673 35009887 215 + 0.763 0.342 -2.003
ENSG00000089351 E016 289.8175917 0.0001633974 2.492586e-01 3.850338e-01 19 35009888 35009972 85 + 2.372 2.395 0.077
ENSG00000089351 E017 297.1506198 0.0003559803 1.834084e-01 3.054824e-01 19 35010092 35010195 104 + 2.378 2.407 0.096
ENSG00000089351 E018 324.5303130 0.0001729728 4.338352e-01 5.762326e-01 19 35010284 35010379 96 + 2.428 2.441 0.045
ENSG00000089351 E019 88.2734996 0.0009121623 1.154219e-09 1.690870e-08 19 35010380 35010639 260 + 1.623 1.930 1.036
ENSG00000089351 E020 134.5042078 0.0025621935 1.205555e-01 2.207614e-01 19 35011474 35011483 10 + 2.112 2.044 -0.226
ENSG00000089351 E021 205.5020955 0.0031818956 5.773864e-02 1.227536e-01 19 35011484 35011554 71 + 2.299 2.224 -0.249
ENSG00000089351 E022 21.8791924 0.0008965645 1.653303e-09 2.353041e-08 19 35012988 35013255 268 + 1.583 1.131 -1.572
ENSG00000089351 E023 222.5992968 0.0005693343 1.846542e-03 6.747977e-03 19 35013256 35013368 113 + 2.344 2.253 -0.305
ENSG00000089351 E024 22.2150235 0.0204574166 2.074990e-04 9.960236e-04 19 35013369 35013540 172 + 1.539 1.169 -1.286
ENSG00000089351 E025 204.1952068 0.0015252805 1.191765e-01 2.187649e-01 19 35013541 35013606 66 + 2.280 2.225 -0.183
ENSG00000089351 E026 242.2482953 0.0003709683 3.094911e-01 4.517951e-01 19 35013607 35013691 85 + 2.335 2.303 -0.105
ENSG00000089351 E027 378.6404772 0.0002358454 9.186850e-01 9.526323e-01 19 35014189 35014387 199 + 2.508 2.502 -0.021
ENSG00000089351 E028 8.5177940 0.0037639721 1.873679e-05 1.169680e-04 19 35014388 35014879 492 + 1.216 0.731 -1.816
ENSG00000089351 E029 12.5353097 0.0081120614 1.846419e-07 1.781324e-06 19 35014969 35015823 855 + 1.397 0.868 -1.911
ENSG00000089351 E030 304.8670631 0.0003956914 9.673238e-01 9.835373e-01 19 35015824 35015967 144 + 2.413 2.408 -0.016
ENSG00000089351 E031 255.7152055 0.0002087267 2.991759e-01 4.406758e-01 19 35019191 35019309 119 + 2.316 2.339 0.075
ENSG00000089351 E032 290.9831885 0.0030835064 7.135882e-01 8.100440e-01 19 35019391 35019533 143 + 2.402 2.388 -0.046
ENSG00000089351 E033 286.9666288 0.0002523496 1.420714e-01 2.508622e-01 19 35021502 35021605 104 + 2.361 2.393 0.106
ENSG00000089351 E034 398.9822524 0.0003215892 6.516988e-03 1.996610e-02 19 35021691 35021864 174 + 2.484 2.540 0.184
ENSG00000089351 E035 325.9488281 0.0035446875 5.526124e-02 1.184392e-01 19 35021951 35022038 88 + 2.390 2.458 0.227
ENSG00000089351 E036 3.6494563 0.0053336513 2.970233e-01 4.383232e-01 19 35022900 35022911 12 + 0.721 0.555 -0.718
ENSG00000089351 E037 326.8750825 0.0011689297 5.247207e-03 1.660349e-02 19 35023236 35023297 62 + 2.385 2.460 0.248
ENSG00000089351 E038 310.8673977 0.0007976797 4.904397e-02 1.074581e-01 19 35023298 35023343 46 + 2.383 2.432 0.163
ENSG00000089351 E039 332.2262952 0.0005290437 3.529005e-05 2.061356e-04 19 35023427 35023547 121 + 2.371 2.471 0.333
ENSG00000089351 E040 9.7983039 0.1191730905 4.844743e-02 1.064179e-01 19 35023548 35023830 283 + 1.187 0.854 -1.228
ENSG00000089351 E041 264.3992878 0.0030735659 4.325028e-03 1.405547e-02 19 35026049 35026471 423 + 2.270 2.374 0.346