ENSG00000089335

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000423823 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding protein_coding 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 2.72952413 0.3033039 3.71779992 0.3033039 1.44360360 3.572690 0.24392500 0.11683333 0.250266667 0.133433333 3.123773e-01 1.687085e-06 FALSE  
ENST00000457781 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding protein_coding 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.72198564 0.3527802 1.06427290 0.3527802 1.06427290 1.566193 0.07810417 0.10403333 0.068900000 -0.035133333 8.447807e-01 1.687085e-06 FALSE  
ENST00000505163 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding protein_coding 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.65704354 0.0000000 1.17145032 0.0000000 0.08556179 6.884415 0.05319583 0.00000000 0.078133333 0.078133333 1.687085e-06 1.687085e-06 FALSE  
ENST00000505242 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding protein_coding 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.65663207 0.3410571 0.46829665 0.2352907 0.46829665 0.446200 0.07247500 0.10753333 0.030300000 -0.077233333 4.529076e-01 1.687085e-06 FALSE  
ENST00000507959 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding protein_coding 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.26996360 0.2470080 0.07463037 0.1243388 0.07463037 -1.602566 0.04683333 0.09983333 0.005066667 -0.094766667 2.259261e-01 1.687085e-06 FALSE  
ENST00000509528 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding protein_coding 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.07943858 0.3173204 0.00000000 0.3173204 0.00000000 -5.032632 0.02177500 0.09356667 0.000000000 -0.093566667 5.134844e-01 1.687085e-06 FALSE  
ENST00000510002 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding retained_intron 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.57053055 0.1542386 0.80012680 0.1542386 0.43672141 2.302355 0.06819167 0.05943333 0.054300000 -0.005133333 8.178763e-01 1.687085e-06    
ENST00000613363 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding protein_coding 9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.81607639 0.2814853 2.28218957 0.1038315 0.34977432 2.975231 0.07495000 0.10520000 0.153266667 0.048066667 6.162714e-01 1.687085e-06 FALSE  
MSTRG.16947.13 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding   9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.96589651 0.2791449 1.31931255 0.2791449 0.16831107 2.200816 0.08907500 0.11890000 0.088333333 -0.030566667 5.811536e-01 1.687085e-06 FALSE  
MSTRG.16947.3 ENSG00000089335 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF302 protein_coding   9.90865 2.778212 14.97202 0.3146587 0.2393496 2.42582 0.59629697 0.3233347 0.91309332 0.3233347 0.65033251 1.469505 0.10322500 0.13773333 0.061433333 -0.076300000 8.712322e-01 1.687085e-06 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000089335 E001 0.0000000       19 34675630 34677130 1501 +      
ENSG00000089335 E002 2.0348834 0.0103025042 5.585875e-01 6.867980e-01 19 34677131 34677550 420 + 0.391 0.502 0.580
ENSG00000089335 E003 0.5975289 0.0276723483 5.449256e-01 6.753313e-01 19 34677610 34677638 29 + 0.200 0.000 -11.032
ENSG00000089335 E004 0.7490443 0.0804939458 4.192080e-01 5.624482e-01 19 34677639 34677646 8 + 0.238 0.000 -11.101
ENSG00000089335 E005 0.7490443 0.0804939458 4.192080e-01 5.624482e-01 19 34677647 34677653 7 + 0.238 0.000 -11.101
ENSG00000089335 E006 1.1887151 0.0149820928 1.817790e-01 3.034621e-01 19 34677654 34677661 8 + 0.336 0.000 -11.989
ENSG00000089335 E007 1.4768704 0.0611013099 1.282845e-01 2.317518e-01 19 34677662 34677677 16 + 0.391 0.000 -12.045
ENSG00000089335 E008 1.6209963 0.1204917331 8.050594e-01 8.759654e-01 19 34677678 34677688 11 + 0.335 0.394 0.342
ENSG00000089335 E009 2.9507314 0.0055976881 4.466970e-01 5.881707e-01 19 34677689 34677691 3 + 0.541 0.390 -0.769
ENSG00000089335 E010 4.9748919 0.0038817507 8.692977e-01 9.200053e-01 19 34677692 34677701 10 + 0.693 0.666 -0.115
ENSG00000089335 E011 5.7218599 0.0029663559 5.942395e-01 7.164980e-01 19 34677702 34677706 5 + 0.753 0.666 -0.360
ENSG00000089335 E012 6.6203433 0.0024966427 3.517273e-01 4.959545e-01 19 34677707 34677716 10 + 0.816 0.666 -0.609
ENSG00000089335 E013 12.5315114 0.0014275616 1.427258e-01 2.517391e-01 19 34677717 34677754 38 + 1.066 0.877 -0.703
ENSG00000089335 E014 17.5493029 0.0010583931 6.019432e-01 7.229845e-01 19 34677755 34677796 42 + 1.180 1.126 -0.195
ENSG00000089335 E015 16.3985476 0.0012172857 1.750388e-01 2.947266e-01 19 34677797 34677833 37 + 1.172 1.019 -0.553
ENSG00000089335 E016 10.2585448 0.0075151133 7.070080e-01 8.050328e-01 19 34677834 34677835 2 + 0.969 0.917 -0.194
ENSG00000089335 E017 12.4729319 0.0016205402 4.127265e-01 5.562941e-01 19 34677836 34677856 21 + 1.055 0.954 -0.374
ENSG00000089335 E018 4.6972465 0.0034255175 5.591460e-01 6.872853e-01 19 34677857 34677862 6 + 0.693 0.592 -0.438
ENSG00000089335 E019 18.2580993 0.0011202740 6.696209e-01 7.763834e-01 19 34677863 34678103 241 + 1.193 1.149 -0.156
ENSG00000089335 E020 34.4890002 0.0006266981 6.322055e-02 1.320763e-01 19 34678737 34678813 77 + 1.477 1.329 -0.512
ENSG00000089335 E021 5.7155197 0.0030210053 3.193839e-02 7.573894e-02 19 34679828 34679959 132 + 0.796 0.390 -1.853
ENSG00000089335 E022 13.6030512 0.0013301978 2.726860e-01 4.116388e-01 19 34681283 34682776 1494 + 1.087 0.954 -0.490
ENSG00000089335 E023 46.9813865 0.0005323147 1.302889e-01 2.345676e-01 19 34682777 34682897 121 + 1.600 1.498 -0.346
ENSG00000089335 E024 7.8276191 0.0021092809 5.607535e-01 6.887027e-01 19 34682898 34683147 250 + 0.870 0.784 -0.335
ENSG00000089335 E025 9.4331011 0.0022649623 6.391657e-01 7.526091e-01 19 34683152 34683154 3 + 0.941 0.877 -0.241
ENSG00000089335 E026 41.1784310 0.0005503693 2.171086e-01 3.469120e-01 19 34683155 34683238 84 + 1.544 1.456 -0.300
ENSG00000089335 E027 6.1645865 0.0028347793 3.926228e-02 8.964907e-02 19 34683992 34684251 260 + 0.706 0.987 1.094
ENSG00000089335 E028 410.1065578 0.0003135732 2.088457e-06 1.618341e-05 19 34684252 34686397 2146 + 2.497 2.564 0.223