ENSG00000089280

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254108 ENSG00000089280 HEK293_OSMI2_2hA HEK293_TMG_2hB FUS protein_coding protein_coding 808.3957 1262.835 586.8767 35.26791 9.163854 -1.105524 356.16617 570.390179 265.62745 20.2891016 3.862926 -1.1025159 0.43506667 0.4518333 0.4530000 0.001166667 1.000000e+00 1.503017e-53 FALSE TRUE
ENST00000380244 ENSG00000089280 HEK293_OSMI2_2hA HEK293_TMG_2hB FUS protein_coding protein_coding 808.3957 1262.835 586.8767 35.26791 9.163854 -1.105524 177.83413 273.764886 137.42603 8.3653574 1.316105 -0.9942298 0.21992500 0.2168667 0.2343667 0.017500000 4.149521e-01 1.503017e-53 FALSE TRUE
ENST00000474990 ENSG00000089280 HEK293_OSMI2_2hA HEK293_TMG_2hB FUS protein_coding processed_transcript 808.3957 1262.835 586.8767 35.26791 9.163854 -1.105524 177.29986 370.369517 74.30173 25.5784782 12.318466 -2.3173425 0.19836667 0.2930000 0.1260000 -0.167000000 2.806499e-05 1.503017e-53 FALSE FALSE
ENST00000487509 ENSG00000089280 HEK293_OSMI2_2hA HEK293_TMG_2hB FUS protein_coding retained_intron 808.3957 1262.835 586.8767 35.26791 9.163854 -1.105524 28.77663 7.434367 39.10140 0.5633868 1.447396 2.3933681 0.04550000 0.0059000 0.0667000 0.060800000 4.293281e-52 1.503017e-53 TRUE TRUE
ENST00000568901 ENSG00000089280 HEK293_OSMI2_2hA HEK293_TMG_2hB FUS protein_coding retained_intron 808.3957 1262.835 586.8767 35.26791 9.163854 -1.105524 35.42768 14.485197 41.81035 1.1179203 3.553481 1.5286304 0.05404583 0.0115000 0.0711000 0.059600000 6.396378e-29 1.503017e-53   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000089280 E001 0.1482932 4.120289e-02 1.000000e+00   16 31179879 31179968 90 + 0.000 0.085 10.406
ENSG00000089280 E002 0.1482932 4.120289e-02 1.000000e+00   16 31179969 31179977 9 + 0.000 0.085 10.381
ENSG00000089280 E003 0.2965864 1.521476e-01 5.802730e-01   16 31179978 31179999 22 + 0.000 0.154 11.363
ENSG00000089280 E004 0.2965864 1.521476e-01 5.802730e-01   16 31180000 31180065 66 + 0.000 0.154 11.363
ENSG00000089280 E005 0.2965864 1.521476e-01 5.802730e-01   16 31180066 31180102 37 + 0.000 0.154 11.363
ENSG00000089280 E006 0.5910844 2.950538e-01 4.800057e-01 6.183342e-01 16 31180103 31180126 24 + 0.296 0.153 -1.214
ENSG00000089280 E007 0.7727888 4.205295e-02 6.587449e-01 7.677705e-01 16 31180127 31180129 3 + 0.296 0.217 -0.595
ENSG00000089280 E008 2.5712351 3.208093e-02 1.346141e-01 2.405713e-01 16 31180130 31180137 8 + 0.691 0.436 -1.175
ENSG00000089280 E009 2.5712351 3.208093e-02 1.346141e-01 2.405713e-01 16 31180138 31180138 1 + 0.691 0.436 -1.175
ENSG00000089280 E010 244.6028821 9.344054e-03 1.073764e-17 5.255010e-16 16 31180139 31180149 11 + 2.618 2.160 -1.527
ENSG00000089280 E011 796.4837539 3.792785e-04 5.482922e-52 3.736414e-49 16 31180150 31180150 1 + 3.012 2.771 -0.799
ENSG00000089280 E012 4267.0746650 1.830052e-03 1.300222e-02 3.591710e-02 16 31180151 31180227 77 + 3.615 3.563 -0.175
ENSG00000089280 E013 4004.3901638 1.598968e-03 1.620956e-02 4.318698e-02 16 31182398 31182422 25 + 3.585 3.537 -0.160
ENSG00000089280 E014 7905.2364348 8.236304e-04 8.836030e-04 3.556848e-03 16 31182513 31182664 152 + 3.879 3.833 -0.153
ENSG00000089280 E015 44.4610968 7.009451e-03 7.020976e-49 3.970456e-46 16 31182665 31183350 686 + 2.080 1.048 -3.551
ENSG00000089280 E016 23.2103321 8.415837e-03 3.247647e-30 5.572980e-28 16 31183550 31183857 308 + 1.800 0.807 -3.520
ENSG00000089280 E017 2332.0200585 5.359915e-04 1.315119e-03 5.027347e-03 16 31183858 31183860 3 + 3.347 3.304 -0.143
ENSG00000089280 E018 8175.7890888 6.221420e-04 8.124568e-01 8.810541e-01 16 31183861 31184002 142 + 3.862 3.859 -0.008
ENSG00000089280 E019 6954.2939073 2.379232e-04 5.902462e-01 7.131356e-01 16 31184209 31184378 170 + 3.793 3.789 -0.011
ENSG00000089280 E020 468.0804674 2.191830e-03 4.697325e-06 3.364693e-05 16 31184379 31184384 6 + 2.711 2.583 -0.427
ENSG00000089280 E021 416.2264907 9.740259e-03 1.483709e-03 5.584159e-03 16 31184385 31184390 6 + 2.681 2.520 -0.535
ENSG00000089280 E022 423.6416184 2.301752e-02 6.944706e-03 2.107946e-02 16 31184391 31184396 6 + 2.715 2.512 -0.676
ENSG00000089280 E023 6.3083093 3.252324e-03 6.212158e-03 1.917296e-02 16 31184397 31184398 2 + 0.471 0.899 1.826
ENSG00000089280 E024 1741.6259367 3.566574e-03 9.822543e-02 1.878263e-01 16 31184939 31185022 84 + 3.225 3.176 -0.163
ENSG00000089280 E025 1641.2987239 6.674269e-03 4.232989e-01 5.663669e-01 16 31185023 31185058 36 + 3.184 3.154 -0.100
ENSG00000089280 E026 1167.3087804 1.326893e-04 1.638396e-16 6.868536e-15 16 31185059 31185079 21 + 3.086 2.987 -0.329
ENSG00000089280 E027 2928.7491526 8.948009e-04 2.826765e-01 4.227095e-01 16 31185080 31185151 72 + 3.426 3.410 -0.055
ENSG00000089280 E028 3101.8154703 1.660764e-03 5.635663e-01 6.910157e-01 16 31185152 31185163 12 + 3.429 3.444 0.049
ENSG00000089280 E029 3502.4002996 1.500912e-04 7.863476e-03 2.343789e-02 16 31185164 31185179 16 + 3.474 3.499 0.085
ENSG00000089280 E030 257.5386342 1.587629e-02 3.343005e-41 1.244248e-38 16 31185180 31185498 319 + 2.808 1.894 -3.053
ENSG00000089280 E031 118.6661376 3.819203e-03 5.086376e-110 2.007497e-106 16 31185499 31185556 58 + 2.505 1.439 -3.591
ENSG00000089280 E032 900.6661426 3.955218e-03 6.104519e-177 1.652118e-172 16 31185557 31186801 1245 + 3.366 2.379 -3.283
ENSG00000089280 E033 4258.8197289 2.414917e-04 1.525710e-01 2.651730e-01 16 31186802 31186836 35 + 3.566 3.581 0.050
ENSG00000089280 E034 994.2036288 4.963898e-03 1.011788e-111 4.166960e-108 16 31186837 31188151 1315 + 3.373 2.530 -2.806
ENSG00000089280 E035 357.6582162 5.760786e-03 3.098100e-40 1.095011e-37 16 31188152 31188324 173 + 2.830 2.271 -1.861
ENSG00000089280 E036 5267.1468659 6.900439e-05 8.324522e-01 8.950178e-01 16 31188325 31188357 33 + 3.669 3.670 0.003
ENSG00000089280 E037 84.7961195 3.148007e-04 3.795836e-105 1.284125e-101 16 31188467 31189119 653 + 2.314 1.449 -2.922
ENSG00000089280 E038 82.9192832 3.162159e-04 1.765830e-08 2.080029e-07 16 31189120 31189122 3 + 2.024 1.799 -0.755
ENSG00000089280 E039 8504.2027429 3.041371e-04 4.744324e-01 6.132586e-01 16 31189123 31189226 104 + 3.872 3.879 0.025
ENSG00000089280 E040 9864.7218947 1.893871e-04 4.397377e-15 1.525490e-13 16 31189665 31189794 130 + 3.897 3.958 0.200
ENSG00000089280 E041 7827.0339645 2.465559e-03 2.845229e-07 2.645757e-06 16 31190040 31190141 102 + 3.748 3.871 0.410
ENSG00000089280 E042 16.0126975 5.333538e-03 1.256955e-01 2.281406e-01 16 31190216 31190274 59 + 1.070 1.230 0.571
ENSG00000089280 E043 4725.0743036 4.486950e-04 1.803264e-29 2.880463e-27 16 31190275 31190331 57 + 3.520 3.655 0.450
ENSG00000089280 E044 3575.8361679 1.202167e-03 1.109712e-07 1.117956e-06 16 31190332 31190361 30 + 3.427 3.526 0.331
ENSG00000089280 E045 3512.0077384 4.209524e-04 1.384675e-20 9.490682e-19 16 31190362 31190372 11 + 3.407 3.522 0.383
ENSG00000089280 E046 4573.4547607 6.435320e-04 9.144954e-16 3.473666e-14 16 31190373 31190398 26 + 3.524 3.636 0.372
ENSG00000089280 E047 15.8905548 9.290893e-03 7.448342e-04 3.060283e-03 16 31190399 31190741 343 + 1.389 1.072 -1.120
ENSG00000089280 E048 5810.8943804 5.877884e-04 2.204718e-05 1.353326e-04 16 31190742 31190827 86 + 3.671 3.727 0.186
ENSG00000089280 E049 3872.0513264 3.912032e-04 1.526624e-02 4.108976e-02 16 31190828 31190842 15 + 3.514 3.543 0.097
ENSG00000089280 E050 4452.2508917 9.868602e-04 3.200226e-01 4.631224e-01 16 31190963 31191079 117 + 3.583 3.601 0.061
ENSG00000089280 E051 2700.8436026 3.031267e-03 2.806422e-06 2.114662e-05 16 31191080 31191110 31 + 3.277 3.412 0.450
ENSG00000089280 E052 4251.9296434 1.032192e-03 7.097881e-13 1.784568e-11 16 31191399 31191804 406 + 3.483 3.606 0.409