ENSG00000089195

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000203001 ENSG00000089195 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT6 protein_coding protein_coding 17.35236 12.00841 25.70584 0.4411723 0.4903609 1.097411 4.296060 3.1652800 6.583710 0.61566515 0.60147092 1.0542067 0.23887083 0.26226667 0.25553333 -0.006733333 0.997493120 0.001633203 FALSE TRUE
ENST00000453074 ENSG00000089195 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT6 protein_coding protein_coding 17.35236 12.00841 25.70584 0.4411723 0.4903609 1.097411 7.347452 6.8911518 10.960056 0.45579004 0.05792389 0.6686618 0.45143750 0.57453333 0.42670000 -0.147833333 0.017511277 0.001633203 FALSE TRUE
ENST00000466974 ENSG00000089195 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT6 protein_coding processed_transcript 17.35236 12.00841 25.70584 0.4411723 0.4903609 1.097411 1.038214 0.2697817 1.627197 0.00527474 0.18302792 2.5488540 0.05986667 0.02256667 0.06326667 0.040700000 0.001633203 0.001633203 TRUE TRUE
MSTRG.20329.5 ENSG00000089195 HEK293_OSMI2_2hA HEK293_TMG_2hB TRMT6 protein_coding   17.35236 12.00841 25.70584 0.4411723 0.4903609 1.097411 4.334115 1.4378478 5.676878 0.83297398 0.44816989 1.9737269 0.23266667 0.12086667 0.22103333 0.100166667 0.665251971 0.001633203 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000089195 E001 2.2301931 0.0076170377 5.467492e-02 1.174283e-01 20 5937228 5937234 7 - 0.616 0.274 -1.836
ENSG00000089195 E002 10.2626185 0.0127435172 1.584859e-01 2.731031e-01 20 5937235 5937832 598 - 1.108 0.948 -0.587
ENSG00000089195 E003 135.7910345 0.0016032875 7.568599e-10 1.147997e-08 20 5937833 5938096 264 - 2.032 2.249 0.727
ENSG00000089195 E004 425.2476348 0.0002935723 5.454433e-05 3.044567e-04 20 5938097 5938726 630 - 2.592 2.664 0.238
ENSG00000089195 E005 137.4232214 0.0002317842 3.189343e-02 7.564748e-02 20 5941053 5941139 87 - 2.105 2.172 0.225
ENSG00000089195 E006 1.9166654 0.0073871309 4.407588e-01 5.824240e-01 20 5941140 5941242 103 - 0.501 0.365 -0.717
ENSG00000089195 E007 135.2674670 0.0002678088 2.942612e-01 4.353696e-01 20 5941243 5941345 103 - 2.114 2.148 0.115
ENSG00000089195 E008 9.7008824 0.0035022392 5.188237e-03 1.644051e-02 20 5941346 5941950 605 - 1.124 0.797 -1.221
ENSG00000089195 E009 140.2764978 0.0002441073 4.681398e-01 6.075623e-01 20 5941951 5942036 86 - 2.150 2.130 -0.066
ENSG00000089195 E010 246.9985592 0.0002101921 9.547184e-03 2.765193e-02 20 5942428 5942674 247 - 2.409 2.352 -0.190
ENSG00000089195 E011 155.7553841 0.0017564850 6.111996e-01 7.303761e-01 20 5942675 5942786 112 - 2.192 2.175 -0.057
ENSG00000089195 E012 151.7680012 0.0002224779 4.863358e-01 6.240846e-01 20 5943559 5943683 125 - 2.166 2.188 0.075
ENSG00000089195 E013 5.2466991 0.0049062060 1.721411e-01 2.909778e-01 20 5943827 5943947 121 - 0.847 0.655 -0.776
ENSG00000089195 E014 103.4690269 0.0009638499 9.353591e-02 1.806514e-01 20 5943948 5944031 84 - 1.984 2.049 0.219
ENSG00000089195 E015 90.3506968 0.0034958828 3.229390e-02 7.641842e-02 20 5944162 5944253 92 - 1.907 2.006 0.333
ENSG00000089195 E016 144.5671809 0.0002447798 8.704298e-03 2.554851e-02 20 5944805 5944914 110 - 2.183 2.106 -0.258
ENSG00000089195 E017 164.0470450 0.0002150456 1.648094e-06 1.305349e-05 20 5946406 5946528 123 - 2.259 2.125 -0.451
ENSG00000089195 E018 67.9553863 0.0004076877 4.116159e-04 1.818974e-03 20 5946529 5946533 5 - 1.887 1.730 -0.531
ENSG00000089195 E019 0.7771569 0.0143489846 4.561702e-01 5.966301e-01 20 5947467 5947572 106 - 0.293 0.158 -1.130
ENSG00000089195 E020 140.0118337 0.0019292708 2.840078e-05 1.698587e-04 20 5950278 5950558 281 - 2.196 2.042 -0.514