ENSG00000089177

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354981 ENSG00000089177 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF16B protein_coding protein_coding 2.622865 1.284813 3.543868 0.07229416 0.1169192 1.456646 1.8977117 0.93639058 2.7853042 0.116314803 0.09192749 1.5624977 0.6953583 0.72436667 0.7868333 0.06246667 0.740112356 0.007957398 FALSE TRUE
ENST00000635823 ENSG00000089177 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF16B protein_coding protein_coding 2.622865 1.284813 3.543868 0.07229416 0.1169192 1.456646 0.2407863 0.26430777 0.2251845 0.001473035 0.04437065 -0.2220023 0.1184542 0.20693333 0.0629000 -0.14403333 0.007957398 0.007957398 FALSE TRUE
ENST00000636835 ENSG00000089177 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF16B protein_coding protein_coding 2.622865 1.284813 3.543868 0.07229416 0.1169192 1.456646 0.3639113 0.06894644 0.4716672 0.068946437 0.10704810 2.6090906 0.1424792 0.05806667 0.1318667 0.07380000 0.454529309 0.007957398 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000089177 E001 58.232588 0.0004832780 6.413982e-09 8.202995e-08 20 16272104 16273252 1149 - 1.635 1.899 0.892
ENSG00000089177 E002 16.954867 0.0327471723 1.683831e-01 2.860801e-01 20 16273253 16273411 159 - 1.162 1.314 0.539
ENSG00000089177 E003 12.594918 0.0026283418 6.675752e-01 7.747686e-01 20 16312335 16312418 84 - 1.108 1.062 -0.166
ENSG00000089177 E004 13.606015 0.0016363770 5.050282e-01 6.408589e-01 20 16335926 16336015 90 - 1.108 1.172 0.230
ENSG00000089177 E005 17.084127 0.0012815476 4.972852e-01 6.338334e-01 20 16356330 16356452 123 - 1.201 1.260 0.209
ENSG00000089177 E006 8.444336 0.0021469323 6.474091e-07 5.599932e-06 20 16366843 16367826 984 - 0.700 1.247 2.055
ENSG00000089177 E007 10.041247 0.0017521023 2.727000e-05 1.638509e-04 20 16367827 16369266 1440 - 0.828 1.260 1.588
ENSG00000089177 E008 14.249710 0.0012954448 1.567423e-01 2.707536e-01 20 16370586 16370636 51 - 1.102 1.233 0.468
ENSG00000089177 E009 16.012457 0.0072409896 3.011582e-01 4.429346e-01 20 16371665 16371761 97 - 1.163 1.260 0.346
ENSG00000089177 E010 0.000000       20 16371762 16371763 2 -      
ENSG00000089177 E011 17.924039 0.0025029415 4.156523e-01 5.590198e-01 20 16374257 16374409 153 - 1.216 1.286 0.246
ENSG00000089177 E012 67.653178 0.0004010631 5.512185e-01 6.807192e-01 20 16378805 16379749 945 - 1.786 1.812 0.087
ENSG00000089177 E013 36.058505 0.0006730474 4.278205e-01 5.706452e-01 20 16379750 16380163 414 - 1.514 1.563 0.165
ENSG00000089177 E014 9.981447 0.0018206245 6.437681e-01 7.562234e-01 20 16381694 16381747 54 - 1.023 0.970 -0.197
ENSG00000089177 E015 0.000000       20 16382098 16382130 33 -      
ENSG00000089177 E016 14.565198 0.0012278638 8.241641e-02 1.633164e-01 20 16404813 16404901 89 - 1.102 1.260 0.564
ENSG00000089177 E017 13.642871 0.0013971723 2.524116e-01 3.887661e-01 20 16406374 16406456 83 - 1.095 1.204 0.388
ENSG00000089177 E018 15.193139 0.0012112900 3.128697e-01 4.553889e-01 20 16427104 16427241 138 - 1.201 1.102 -0.353
ENSG00000089177 E019 11.308177 0.0016836715 5.432843e-02 1.168232e-01 20 16428953 16429004 52 - 1.108 0.884 -0.829
ENSG00000089177 E020 14.252437 0.0012790263 3.319755e-02 7.819246e-02 20 16429863 16429982 120 - 1.196 0.970 -0.819
ENSG00000089177 E021 12.252912 0.0424084327 3.383609e-01 4.822305e-01 20 16494291 16494350 60 - 1.114 0.976 -0.506
ENSG00000089177 E022 12.549629 0.0017276581 3.502997e-02 8.170699e-02 20 16497613 16497678 66 - 1.151 0.915 -0.868
ENSG00000089177 E023 19.750473 0.0010552001 2.056162e-02 5.267386e-02 20 16504372 16504547 176 - 1.331 1.121 -0.742
ENSG00000089177 E024 16.200614 0.0014813360 1.809836e-01 3.024568e-01 20 16505722 16505853 132 - 1.231 1.102 -0.460
ENSG00000089177 E025 16.966144 0.0010959830 1.534943e-01 2.664135e-01 20 16506022 16506190 169 - 1.255 1.121 -0.476
ENSG00000089177 E026 14.609743 0.0016369835 2.274996e-01 3.594168e-01 20 16507958 16508100 143 - 1.185 1.063 -0.440
ENSG00000089177 E027 16.940638 0.0045615032 2.600238e-02 6.395089e-02 20 16511418 16511527 110 - 1.269 1.041 -0.813
ENSG00000089177 E028 17.735908 0.0274049310 2.811500e-02 6.824305e-02 20 16512826 16512923 98 - 1.299 1.019 -1.003
ENSG00000089177 E029 17.851418 0.0036639970 9.982100e-04 3.951850e-03 20 16515548 16515664 117 - 1.311 0.970 -1.225
ENSG00000089177 E030 15.122444 0.0012029905 2.409944e-03 8.491720e-03 20 16526092 16526205 114 - 1.241 0.915 -1.185
ENSG00000089177 E031 10.965469 0.0015309885 2.283720e-03 8.101245e-03 20 16528371 16528440 70 - 1.121 0.736 -1.459
ENSG00000089177 E032 10.145448 0.0017388103 1.835851e-02 4.791839e-02 20 16573229 16573448 220 - 1.075 0.778 -1.122